Rapid analysis of loratadine in human serum by high-performance liquid chromatography with fluorescence detection

• Autorzy: Plenis A. , Konieczna L. , Olędzka I , Kowalski P.
• Języki publikacji: EN

Abstrakty

EN

A simple and rapid high-performance liquid chromatographic method with fluorescence detection for analysis of loratadine (LOR) in small volumes of human serum has been developed and validated. After solid-phase extraction (SPE), with thioridazine hydrochloride as internal standard, chromatographic separation was performed on a C 18 analytical column with 70:30 ( v / v ) acetonitrile-water, adjusted to pH 2.7 with orthophosphoric acid as mobile phase at a flow-rate of 1 min mL ^-1. The column was maintained at 28°C. Fluorescence detection was performed at excitation and emission wavelengths of 265 of 454 nm, respectively. The method was validated for accuracy, precision, selectivity, linearity, recovery, and stability. Absolute recovery of LOR was >93.0%. The limits of detection (LOD) and quantification (LOQ) were 0.07 and 0.2 ng mL ^-1,respectively. Linearity was confirmed in the range 0.2–30 ng mL ^-1 (correlation coefficient >0.9998). This HPLC method is selective, robust, and specific and would enable efficient analysis of large numbers of serum samples in support of pharmacokinetic, bioavailability, or bioequivalence studies after therapeutic doses of LOR.

Słowa kluczowe

EN

loratadine; serum; HPLC; fluorescence detection; determination

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2010
• Tom: Vol. 22, no. 1
• Strony: 69--79
• Opis fizyczny: Bibliogr. 14 poz., rys., tab.

Twórcy

autor

Plenis A.

• Medical University of Gdańsk Department of Pharmaceutical Chemistry Hallera 107 80-416 Gdańsk Poland

autor

Konieczna L.

• Medical University of Gdańsk Department of Pharmaceutical Chemistry Hallera 107 80-416 Gdańsk Poland

autor

Olędzka I

• Medical University of Gdańsk Department of Pharmaceutical Chemistry Hallera 107 80-416 Gdańsk Poland

autor

Kowalski P.

• Medical University of Gdańsk Department of Pharmaceutical Chemistry Hallera 107 80-416 Gdańsk Poland

Bibliografia

• [1] W. Kreunter, R.W. Chapman, A. Gulbenkain, and M.I. Siegel, Allergy, 42 , 57 (1987)
• [2] S.P. Clissold, E.M. Sorkin, and K.L. Gao, Drugs, 37 , 42 (1989)
• [3] D.I. Sora, S. Udrescu, V. David, and A. Medvedovici, Biomed. Chromatogr., 21 , 1023 (2007)
• [4] F.C. Sutherland, A.D. de Jager, D. Badenhorst, T. Scanes, H.K. Hundt, K.J. Swart, and A.F Hundt, J. Chromatogr. A, 914 , 37 (2001)
• [5] H. Amini, J. Chromatogr. B, 809 , 227 (2004)
• [6] I.I. Salem, J. Idrees, and J.I. Al Tamimi, J. Pharm. Biomed. Anal., 34 , 141 (2004)
• [7] P.K. Kunicki, J. Chromatogr. B, 755 , 331 (2001)
• [8] L. Vlase, S. Imre, D. Muntean, and S.E. Leucuta, J. Pharm. Biomed. Anal., 44 , 652 (2007)
• [9] O.Q.P. Yin, X. Shi, and M.S.S. Chow, J. Chromatogr. B, 796 , 165 (2003)
• [10] J. Sun, G. Wang, W. Wang, S. Zhao, Y. Gu, J. Zhang, M. Huang, F. Shao, H. Li, Q. Zhang, and H. Xie, J. Pharm. Biomed. Anal., 39 , 217 (2005)
• [11] J. Martens, J. Chromatogr. B, 673 , 183 (1995)
• [12] S. Emara, A. El-Gindy, M.K. Mesbah, and G.M. Hadad, J. AOAC Int., 90 , 384 (2007)
• [13] M.E. Capella-Peiro, A. Bossi, and J. Esteve-Romero, Anal. Biochem., 352 , 41 (2006)
• [14] ICH Note for Guidelines on Validation of Analytical Procedures: Text and Methodology (Q2R1), IFPMA, Switzerland, 1995