Target-guided isolation and purification of antioxidants from Elsholtzia densa benth. var. densa by DPPH antioxidant assay and dual-mode HSCCC

• Autorzy: Zhou W. N. , Ouyang J. , Wang Z. H. , Wang X. Y. , Suo Y. R. , Zhang Z. , Wang H. L.

Identyfikatory

DOI

10.1556/1326.2017.00192

• Języki publikacji: EN

Abstrakty

EN

Elsholtzia densa Benth. var. densa (Lamiaceae) is a famous medicinal herb which has been widely used for treatment of colds, headaches, pharyngitis, fever, diarrhea, digestion disorder, rheumatic arthritis, nephritises, and nyctalopia in China. In this study, fraction of the ethyl alcohol extract of E. densa (aerial part) by different polarity solvents indicated that the ethyl acetate soluble fraction exhibited a potent 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging activity with the IC50 value of 148.2 μg/mL. Under the target guidance of DPPH experiment, isoquercitrin, trachelogenin, ethyl caffeate, and arctigenin were separated with purities 95.98%, 92.98%, 96.07%, and 88.83%, respectively, by a dual-mode high-speed counter-current chromatography (HSCCC) method using n-hexane–ethyl acetate–methanol–water (4.5:5:3:4, v/v/v/v) as the solvent system. In order to evaluate the scientific basis, antioxidant activity of four isolated compounds was assessed by the radical scavenging effect on DPPH radical; isoquercitrin and ethyl caffeate showed stronger antioxidant activities with IC50 values of 9.4 μg/mL and 9.2 μg/mL, respectively, while trachelogenin and arctigenin showed weak antioxidant activities with IC50 values of >500 μg/mL and 72.8 μg/mL, respectively. Results of the present study indicated that the combinative method using DPPH antioxidant assay and dual-mode HSCCC could be widely applied for rapid screening and isolating of antioxidants from complex traditional Chinese medicine extract.

Słowa kluczowe

EN

Elsholtzia densa Benth; var; densa; antioxidant; target-guided; dual-mode HSCCC

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2018
• Tom: Vol. 30, no. 3
• Strony: 180--185
• Opis fizyczny: Bibliogr. 33 poz.

Twórcy

autor

Zhou W. N.

• Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China
• University of the Chinese Academy of Sciences, Beijing 100049, China

autor

Ouyang J.

• Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China
• University of the Chinese Academy of Sciences, Beijing 100049, China

autor

Wang Z. H.

• College of Life Sciences, Yantai University, Yantai 264025, China

autor

Wang X. Y.

• Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China

autor

Suo Y. R.

• Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China

autor

Zhang Z.

• JALA Co. Ltd., Shanghai 200233, China

autor

Wang H. L.

• Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China

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Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2018).