Analysis of scopolamine and its related substances by means of high-performance liquid chromatography

• Autorzy: Obradovic Darija , Pešic Ivana , Carapic Marija , Lazovic Saša , AGBABA Danica

Identyfikatory

DOI

10.1556/1326.2022.01107

• Języki publikacji: EN

Abstrakty

EN

The retention behaviour of scopolamine (hyoscine) and its related compounds (norhyoscine, atropine, homatropine, and noratropine) was investigated on the silica-based HPLC stationary phase. The retention of investigated tropane alkaloids was interpreted by using the Soczewinski-Wachtmeister equation. A high correlation between the retention parameter (log k) and lipophilicity (log P) (R 5 0.9923) confirms the significant influence of hydrophobic interactions on the retention behaviour of the aforementioned compounds. It was found that by increasing the acetonitrile fraction, a decrease in retention of the more polar epoxide derivatives (scopolamine, norhyoscine) and an increase in retention of the more lipophilic derivatives (atropine, noratropine, homatropine) is obtained. The best separation of the tropane alkaloids was achieved by a simple procedure that involved a mobile phase composed of acetonitrile and 40mM ammonium acetate/0.05% TEA, pH 6.5; 50:50 v/v. Selected conditions were assumed for the determination of scopolamine hydrochloride in the eye drops (Scopolamini hydrobromidum 0.25%). The method was validated and it was found as selective, sensitive, precise, accurate, and robust for the further qualitative analysis of the scopolamine-related compounds.

Słowa kluczowe

EN

tropane alkaloids; related substances; method development; eye drops; HPLC

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2024
• Tom: Vol. 36, no. 2
• Strony: 125--131
• Opis fizyczny: Bibliogr. 30 poz., rys., wykr.

Twórcy

autor

Obradovic Darija

• Institute of Physics Belgrade, University of Belgrade, Pregrevica 118, 11080 Belgrade, Serbia

• https://orcid.org/0000-0001-5678-034X

autor

Pešic Ivana

• Service for the Pharmaceutical Business and Supply, Clinical Center of Serbia, Pasterova 2, 11000 Belgrade, Serbia

autor

Carapic Marija

• Medicines and Medical Devices Agency of Serbia, Vojvode Stepe 458, 11000 Belgrade, Serbia

autor

Lazovic Saša

• Institute of Physics Belgrade, University of Belgrade, Pregrevica 118, 11080 Belgrade, Serbia

autor

AGBABA Danica

• Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia

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