Pharmacokinetic study on hirsutine and hirsuteine in rats using UPLC–MS/MS

• Autorzy: Han Aixia , Lin Guanyang , Cai Jinzhang , Wu Qing , Geng Peiwu , Ma Jianshe , Wang Xianqin , Lin Chongliang

Identyfikatory

DOI

10.1556/1326.2017.00365

• Języki publikacji: EN

Abstrakty

EN

An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was established to determine hirsutine and hirsuteine in rat plasma. Pharmacokinetics of hirsutine and hirsuteine in rats after intravenous or oral administration has been investigated using this developed UPLC–MS/MS method, and bioavailability of the two drugs was calculated. Diazepam was used as internal standard, and UPLC BEH column (2.1 mm × 50 mm, 1.7 μm) was used at temperature of 40 °C. The mobile phase was composed of acetonitrile and water (containing 0.1% formic acid) at a gradient elution flow rate of 0.4 mL/min. Nitrogen was used as desolvation gas (800 L/h) and conical gas (50 L/h). The multiple reaction monitoring (MRM) model was applied to quantitatively analyze hirsutine m/z 369 → 226, hirsuteine m/z 367 → 169.9, and diazepam (internal standard) m/z 285.1 → 193.3. Rat plasma samples were deproteinized using acetonitrile prior to UPLC–MS/MS analysis. Within the concentration range of 1–200 ng/mL, the linearity of hirsutine and hirsuteine in plasma was good (r > 0.995), and the lower limit of quantitation was 1 ng/mL. Relative standard deviations of intra-day precision for hirsutine and hirsuteine were ≤6.1% and ≤5.9%, respectively, and those of inter-day precision were ≤6% and ≤7.7%. Accuracy for hirsutine and hirsuteine ranged between 92.3% and 104.8%. Bioavailability of hirsutine and hirsuteine was 4.4% and 8.2%, respectively. The method is sensitive and fast with good selectivity and was successfully applied in the pharmacokinetic studies after intravenous and oral administration of hirsutine and hirsuteine.

Słowa kluczowe

EN

Hirsutine; hirsuteine; pharmacokinetics; UPLC–MS/MS; bioavailability

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2019
• Tom: Vol. 31, no. 2
• Strony: 99--104
• Opis fizyczny: Bibliogr. 37 poz., rys., tab.

Twórcy

autor

Han Aixia

• Department of Pharmacy, The People's Hospital of Lishui, Lishui 323000, China

autor

Lin Guanyang

• Department of Pharmacy, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China

autor

Cai Jinzhang

• The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China

autor

Wu Qing

• Department of Pharmacy, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China

autor

Geng Peiwu

• Department of Pharmacy, The People's Hospital of Lishui, Lishui 323000, China

autor

Ma Jianshe

• Analytical and Testing Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China

autor

Wang Xianqin

• Analytical and Testing Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China

autor

Lin Chongliang

• Department of Pharmacy, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China

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Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).