Oxidative stress enzymes in tobacco during a long-term exposure to ambient ozone at two different sites

Bandurska, H.; Borowiak, K.; Zielezińska, M.

doi:10.24425/122297

Artykuł - szczegóły

Tytuł artykułu

Oxidative stress enzymes in tobacco during a long-term exposure to ambient ozone at two different sites

Autorzy

Bandurska H. , Borowiak K. , Zielezińska M.

Treść / Zawartość

Pełne teksty:

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Identyfikatory

DOI

10.24425/122297

Warianty tytułu

Kumulatywny wpływ ozonu na aktywność enzymów antyoksydacyjnych u tytoniu rosnącego na dwóch stanowiskach ekspozycyjnych

Języki publikacji

Abstrakty

Tropospheric ozone is a harmful air pollutant which may cause oxidative stress in plant cells, leading to biochemical and physiological changes and yield reduction. The aim of the study was to examine the cumulative effect of long-term ozone stress on the activity of antioxidative enzymes in leaves of ozone sensitive and resistant tobacco cultivar growing at the sites of various ozone concentrations. The one-month experiment was conducted in the 2009, 2010 and 2011 growing seasons with different meteorological conditions and ozone concentrations. The activity of SOD, APX and GuPX was measured four times at weekly intervals. The highest tropospheric ozone concentration was recorded in 2010 along with high level of solar radiation and temperature. The enhanced ozone level caused the increase in the activity of all examined enzymes. However, at more elevated ozone level the activity of all enzymes was higher in sensitive (Bel W3) than resistant (Bel B) tobacco cultivar. On the other hand, at lower ozone level the activity of examined enzymes was rather similar in both cultivars or even higher in ozone-resistant one, which was especially valid for SOD. A positive correlation between the activity of all enzymes and ozone concentration was shown in both cultivars. The presented results show that meteorological conditions modify tropospheric ozone concentration and real plant response to this environmental pollutant. It is highly important to interpret week-by-week plant response and environmental conditions bearing in mind the cumulative ozone effect resulting from previous weeks conditions.

Zwiększone stężenie ozon troposferycznego w powietrzu jest szkodliwe dla roślin, gdyż przyczynia się do powstania stresu oksydacyjnego prowadzącego do zmian biochemiczno-fizjologicznych oraz redukcji plonów. Celem pracy było zbadanie wpływu długotrwałego stresu ozonowego na aktywność enzymów antyoksydacyjnych w liściach wrażliwej oraz odpornej na ozon odmiany tytoniu rosnących na stanowiskach ekspozycyjnych różniących się stężeniem tego zanieczyszczenia. Przeprowadzono trzy trwające jeden miesiąc doświadczenia w latach 2009, 2010 i 2011 charakteryzujących się różnymi warunkami meteorologicznymi oraz różnym stężeniem ozonu. Aktywność SOD, APX oraz GuPX oznaczano czterokrotnie (w odstępach tygodniowych) w czasie ekspozycji roślin. Najwyższe stężenie ozonu stwierdzono w roku 2010, kiedy odnotowano również wysoki poziom promieniowania słonecznego oraz temperatury powietrza. Zwiększone stężenie ozonu przyczyniło się do wzrostu aktywności badanych enzymów. Większą aktywność enzymów w warunkach podwyższonego stężenia ozonu stwierdzono u wrażliwej (Bel W3) niż odpornej (Bel B) odmiany tytoniu. Przy niższym stężeniu ozonu, aktywność badanych enzymów była zbliżona u obu odmian, lub nawet wyższa u odmiany odpornej, co było szczególnie widoczne w przypadku SOD. U obu odmian stwierdzono pozytywną korelacje pomiędzy aktywnością badanych enzymów oraz stężeniem ozonu. Uzyskane wyniki wskazują, że warunki atmosferyczne wpływają na stężenie ozonu troposferycznego, co z kolei wpływa na rzeczywistą odpowiedź rośliny na to gazowe zanieczyszczenie powietrza. Niezwykle ważne jest, aby uzyskane dane dotyczące odpowiedzi roślin na zanieczyszczenie powietrza ozonem interpretować z uwzględnieniem czasowych zależności, w szczególności efektu kumulowania się wpływu ozonu w poprzedzającym przedziale czasowym.

Słowa kluczowe

tobacco ozone superoxide dismutase peroxidases

tytoń ozon dysmutaza ponadtlenkowa peroksydazy

Wydawca

Institute of Environmental Engineering, Polish Academy of Sciences

Czasopismo

Archives of Environmental Protection

Rocznik

2018

Tom

Vol. 44, no. 4

Strony

3--11

Opis fizyczny

Bibliogr. 40 poz., wykr.

Twórcy

autor

Bandurska H.

bandur@up.poznan.pl

Poznan University of Life Sciences, Poland, Department of Plant Physiology

autor

Borowiak K.

Poznan University of Life Sciences, Poland, Department of Plant Physiology

autor

Zielezińska M.

Poznan University of Life Sciences, Poland, Department of Plant Physiology

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Uwagi

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).

Typ dokumentu

Bibliografia

Identyfikator YADDA

bwmeta1.element.baztech-3ce72678-3518-4997-b963-8f665f7e8ec2