Development and validation of a densitometric HPTLC method for simultaneous analysis of wedelolactone and asiaticoside in a polyherbal formulation

• Autorzy: Sathiyanarayanan L , Paradkar A. R. , Mahadik K. R.
• Języki publikacji: EN

Abstrakty

EN

A sensitive, accurate, and robust high-performance thin-layer chromatographic (HPTLC) method has been established for simultaneous analysis of wedelolactone (WED) and asiaticoside (ASI) in Eclipta alba and Centella asiatica Linn., respectively. Chromatography was performed on silica gel with toluene-acetone-methanol-formic acid 3.0:2.0:2.0:0.05 ( υ/υ ) as mobile phase. Densitometric scanning at 317 nm for WED and at 530 nm, after derivatisation with 10% methanolic sulphuric acid, for ASI was used. The method was validated in accordance with the guidelines of the International Conference on Harmonization (ICH). R F values of 0.26 and 0.75 were obtained for ASI and WED, respectively. The linear ranges were 50–250 and 150–550 ng per band for WED and ASI, respectively, with good correlation coefficients ( r 2 = 0.999 and 0.9989, respectively). Accuracy was 99.29% and 99.45% for WED and ASI, respectively. The method was found to be precise, robust, and suitable for routine quality-control analysis of plant extracts and polyherbal formulations.

Słowa kluczowe

EN

wedelolactone; asiaticoside; HPTLC; Centella asiatica Linn; Eclipta alba

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2010
• Tom: Vol. 22, no. 4
• Strony: 651--663
• Opis fizyczny: Bibliogr. 21 poz., rys., tab.

Twórcy

autor

Sathiyanarayanan L

• Bharati Vidyapeeth University, Poona College of Pharmacy Department of Quality Assurance Techniques, Faculty of Pharmaceutical Sciences Erandwane, Pune 411 038 Maharashtra India

autor

Paradkar A. R.

• University of Bradford Department of Pharmaceutical Engineering Science, Institute of Pharmaceutical Innovation, and IRC in Polymer Science and Technology Bradford West Yorkshire UK

autor

Mahadik K. R.

• Bharati Vidyapeeth University, Poona College of Pharmacy Department of Quality Assurance Techniques, Faculty of Pharmaceutical Sciences Erandwane, Pune 411 038 Maharashtra India

Bibliografia

• [1] P.A. Melo and C.L. Ownby, Toxicon, 37 , 199 (1999)
• [2] H. Wagner, B. Geyer, Y. Kiso, H. Hikino, and G.S. Rao, Planta Med., 52 , 370 (1986)
• [3] N. Das, G.C. Bhavsar, and M.G. Chauhan, Indian Drugs, 28 , 100 (1990)
• [4] M.B. Patel and S.H. Mishra, Pharmacognosy, 2 , 168 (2006)
• [5] V. Tyler, L. Brady, and J. Robbers, Pharmacognosy, 8th edn, PA: Lea & Febiger, Philadelphia, 1981, pp. 141–142
• [6] J. Bradwejn, Y. Zhou, D. Koszycki, J. Shlik, J. Clin. Psychopharmacol., 20 , 680 (2000)
• [7] D. Winston and S. Maimes, Adaptogens: Herbs For Strength, Stamina, and Stress Relief, Healing Arts Press, Rochester, VT, 2007, pp. 226–227
• [8] T. Kartnig, Herbs, Spices Med. Plants, 3 , 146, (1988)
• [9] B. Diallo, R. Vanhaelenfastre, and M. Vanhaelen, J. Chromatogr., 558 , 446 (1991)
• [10] Q. Du, G. Jerz., P. Chen, and P. Winterhalter, J. Liq. Chromatogr. Relat. Technol., 27 , 2201 (2004)
• [11] M. Bonfill, S. Mangas, R.M. Cusido, L. Osuna, P.M. Teresa, and J. Palazon, Biomed. Chromatogr., 20 , 151 (2006)
• [12] A.P. Gupta, M.M. Gupta, and S. Kumar, J. Ind. Chem. Soc., 76 , 321 (1999)
• [13] P. Rai and S.H. Mishra, Pharmacognosy, 3 , 47 (2007)
• [14] M.N. Ravishankara, N. Srivastava, M.G. Jayathirtha, H. Padh, and M. Rajani, J. Chromatogr. B, 744 , 257 (2000)
• [15] H. Agrawal, N. Kaul, A.R. Paradkar, and K.R. Mahadik, Chromatographia, 62 , 183 (2005)
• [16] WHO, Guidelines for the Assessment of Herbal Medicine, Munich, World Health Organization, 1991
• [17] ICH, Q2A Validation of Analytical Procedure: Methodology, International Conference on Harmonization, Geneva, October, 1994
• [18] ICH, Q2B Validation of Analytical Procedure: Methodology, International Conference on Harmonization, Geneva, March, 1996
• [19] ICH Guidance on Analytical Method Validation, International Convention on Quality for the Pharmaceutical Industry, Toronto, Canada, September, 2002
• [20] P.D. Sethi, High Performance Thin Layer Chromatography, Quantitative Analysis of Pharmaceutical Formulations, CBS Publishers and Distributors, New Delhi, 1996
• [21] M.B. Patel, V.M. Kadakia, and S.H. Mishra, Indian J. Pharm. Sci., 70 , 689 (2008)