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Optimization of Chitin Extraction from Shrimp Shells Using Full Factorial Design Methodology

Treść / Zawartość
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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
This study aims to optimize chitin extraction conditions by reducing water and chemical consumption using a twolevel factorial design approach. Two variables were studied for the demineralization process: the volume of hydrochloric acid (400 to 500 mL) and the demineralization time (1 to 1.5 hours) at room temperature. For deproteinization, the variables examined were NaOH concentration (0.6 to 1 mol/L) and deproteinization time (1 to 1.5 hours) at 70–80 °C. The ash and protein contents were measured to evaluate the efficiency of the demineralization and deproteinization processes, respectively. Minitab 18 software was used for the experimental design. The results showed that the best conditions for extracting high-quality chitin are a demineralization time of 1 hour, an HCl volume of 500 mL, a NaOH concentration of 1 M, and a deproteinization time of 1 hour. These optimal conditions reduce the amount of water, time, and chemicals required for chitin extraction. Compared to previous studies, we significantly reduced the amount of water and chemicals as well as the extraction duration. These results are innovative as they offer a more efficient and sustainable solution for chitin extraction, thus filling a gap in current research by proposing an optimized method. By providing these new data and demonstrating their effectiveness, our research makes a significant contribution to improving chitin extraction techniques, addressing an unmet need in the industrial field.
Twórcy
  • Laboratory of Physico-Chemistry Materials, Natural Substances and Environment, Department of Chemistry, Faculty of Sciences and Techniques, Abdel Malek Essaadi University, Tangier, Morocco
  • Laboratory of Physico-Chemistry Materials, Natural Substances and Environment, Department of Chemistry, Faculty of Sciences and Techniques, Abdel Malek Essaadi University, Tangier, Morocco
  • Laboratory of Physico-Chemistry Materials, Natural Substances and Environment, Department of Chemistry, Faculty of Sciences and Techniques, Abdel Malek Essaadi University, Tangier, Morocco
  • Laboratory of Physico-Chemistry Materials, Natural Substances and Environment, Department of Chemistry, Faculty of Sciences and Techniques, Abdel Malek Essaadi University, Tangier, Morocco
autor
  • Laboratory of Physico-Chemistry Materials, Natural Substances and Environment, Department of Chemistry, Faculty of Sciences and Techniques, Abdel Malek Essaadi University, Tangier, Morocco
  • Laboratory of Organic Chemistry, Catalysis and Environment, Department of Chemistry, Faculty of Sciences, Ibn Tofaïl University, P.O. Box 14000, Kenitra, Morocco
  • Laboratory of Advanced Materials and Process Engineering, Department of Chemistry, Faculty of Sciences, Ibn Tofaïl University, P.O. Box 14000, Kenitra, Morocco
  • Laboratory of Advanced Materials and Process Engineering, Department of Chemistry, Faculty of Sciences, Ibn Tofaïl University, P.O. Box 14000, Kenitra, Morocco
  • Laboratory of Chemistry, Environment and Chemistry of Solid Minerals, Department of Chemistry, Faculty of Sciences, Mohamed the First University, P.O. Box 60000, Oujda, Morocco
  • Research Team of Biotechnology and Biomolecular Engineering, Faculty of Sciences and Techniques, Abdel Malek Essaadi University, Tangier, Morocco
  • Laboratory of Physico-Chemistry Materials, Natural Substances and Environment, Department of Chemistry, Faculty of Sciences and Techniques, Abdel Malek Essaadi University, Tangier, Morocco
  • University Department, Royal Naval School, Boulevard Sour-Jdid, P.O. Box 20052,Casablanca-Morocco
  • Laboratory of Pesticide Residues, Research Unit on Nuclear Techniques, Environment and Quality, INRA Tangier, Morocco
  • Laboratory of Physico-Chemistry Materials, Natural Substances and Environment, Department of Chemistry, Faculty of Sciences and Techniques, Abdel Malek Essaadi University, Tangier, Morocco
Bibliografia
  • 1. Rissouli, L., Benicha, M., Chabbi, M. 2016. Contribution to the elimination of Linuron by the adsorption process using chitin and chitosan biopolymers. J. Mater. Environ. Sci, 7(2), 531–540.
  • 2. Pakizeh, M., Moradi, A., Ghassemi, T. 2021. Chemical extraction and modification of chitin and chitosan from shrimp shells. European Polymer J, 159, 110709. DOI: 10.1016/j.eurpolymj. 110709.
  • 3. Rissouli, L., Benicha, M., Chafik, T., Chabbi, M. 2017. Decontamination of water polluted with pesticide using biopolymers: Adsorption of glyphosate by chitin and chitosan, J. Mater. Environ. Sci, 8(12), 4544–4549. DOI: 10.26872/jmes.2017.8.12.479.
  • 4. Islam, N., Hoque, M., Taharat, S.F. 2023. Recent advances in extraction of chitin and chitosan. World J. Microbiol. Biotechnol, 39. DOI: /10.1007/s11274-022-03468-1.
  • 5. El Knidri, H., Belaabed, R., Addaou, A., Laajeb, A., Lahsini, A. 2018. Extraction, chemical modification and characterization of chitin and chitosan. Inter. J. Bio. Macromolecules, 120, 1181–1189.
  • 6. Tolaimate, A., Desbrieres, J., Rhazi, M., Alagui, A. 2003. Contribution to the preparation of chitins and chitosans with controlled physico-chemical properties. Polymer, 44(2003), 7939–7952. DOI: 10.1016/j.polymer.2003.10.025.
  • 7. Adeyi, A.A., Oloje, A.O., Giwa., A. 2017. Statistical optimization of chitosan extraction from shrimp shells using response surface methodology. Abuad J. Eng. Res. Dev, 1(1), 8–17.
  • 8. Amoo, K.O., Olafadehan, O.A., Ajayi T.O. 2019. Optimization studies of chitin and chitosan production from Penaeus notialis shell waste. Afr. J. Biotechnol, 18(27), 670–688. DOI: 10.5897/AJB2019.16861.
  • 9. Al Hoqani, H.A.S., AL-Shaqsi, N., Hossain M.A., Al Sibani. M.A. 2020. Isolation and optimization of the method for industrial production of chitin and chitosan from Omani shrimp shell. 2020. Carbohydr. Res, 492. DOI: 10.1016/j.wen.2021.11.002.
  • 10. Firzanah, H., Maziati, M.H., Ahmad, F., Kartini, A., Samat, N. 2024. Biopolymer chitosan: Potential sources, extraction methods, and emerging applications. Ain Shams Eng. J, 15(2), 102424. DOI: S2090447923003131.
  • 11. Puvvada, Y.S., Vankayalapati, S., Sukhavasi, S. 2012. Extraction of chitin from chitosan from exoskeleton of shrimp for application in the pharmaceutical industry. Int. Curr. Pharm. J, 1(9), 258–263.
  • 12. Gornall, A.G., Bardwill, C.J., Davidd, M.M. 1948. Determination of serum proteins by means of the Biurret reaction. J. Biol. Che, 751–766.
  • 13. Lin, J., Wang, L., Wang, L. 2012. Coagulation of Sericin Protein in Silk Degumming Wastewater Using Quaternized Chitosan. J. Polym. Environ, 20, 858–864. DOI: 10.1007/s10924-012-0444-3.
  • 14. Yapa, P.A.J., Silva, A., Senarat, W.T.P.S.K. 2009. Use of dried papaya milk in chitosan manufacture. Vidvodaya J. Sci., 14, 31–39. DOI: dr.lib.sjp.ac.lk/handle/123456789/1089.
  • 15. Eddaoukhi, A., Berradi, M., El Rhayam, Y., Rissouli, L., Elyacoubi, A., Eddaoukhi, A., Berradi,O., Sallek, B., El Bachiri. A., Nassali. H. 2023. Aerated lagoon/adsorption combination method for the treatment of olive mill wastewater: optimizing parameters using study design. Enviro. Monitoring and Assessment, 195, 1111. DOI: 10.1007/s10661-023-11726-4.
  • 16. Eddaoukhi, A., Berradi, M., El Rhayam, Y., Rissouli, L., Grou, M., Elyacoubi, A., Bouraada. K., Zerrouk, M.H., El Bachiri, A.H., Nassali, A.H. 2024. Characterizing and optimizing adsorption for olive mill wastewater processing in Loukkos, Morocco. Enviro. Monitoring and Assessment, 196, 125. DOI: 10.1007/s10661-023-12179-5.
  • 17. Bajaj, M., Winter, J., Galler, C. 2011. Effect of deproteination and deacetylation conditions on viscosity of chitin and chitosan extracted from Crangon shrimp waste. Biochem. Eng. J, 56(1–2), 51–62. DOI : 10.1016/j.bej.2011.05.006.
  • 18. Benhabiles, M.S., Drouiche, N., Lounici, H., Pauss, A., Mameri, N. 2013. Effect of shrimp chitosan coatings as affected by chitosan extraction processes on postharvest quality of strawberry. Measurement Science and Technology, 7, 215–221. DOI : 10.1007/s11694-013-9159-y.
  • 19. Tokatlı, K., Demirdoven, A. 2017. Optimization of chitin and chitosan production from shrimp wastes and characterization. J. Food Process Preserv. DOI: 10.1111/jfpp.13494.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-3ac6cbdd-c6f7-422b-ac78-733c90dd3110
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