Validated capillary zone electrophoretic method for simultaneous analysis of benazepril in combination with amlodipine besylate and hydrochlorothiazide

• Autorzy: Ahmed Hytham M. , Belal Tarek S. , Shaalan Rasha A. , Yazbi Fawzy A. , Elonsy Sohila M.

Identyfikatory

DOI

10.1556/1326.2019.00686

• Języki publikacji: EN

Abstrakty

EN

In this work, a novel, simple, and quick capillary zone electrophoresis (CZE) method was proposed for simultaneous analysis of benazepril (BEN) with other co-administrated antihypertensive drugs, amlodipine besylate (AML) and hydrochlorothiazide (HCT), using a diode array detector (DAD). A fused silica capillary (78.5 cm total length, 70 cm effective length, and 75 μm id) was used in separation using a 40 mM phosphate buffer pH 7.5 as a running background electrolyte (BGE) under a positive potential of 30 KV, at a stable temperature of 25 °C for capillary during separation. Hydrodynamic injections were performed for 12 s at 50 mbar, and detection was performed at 210 nm for AML and BEN, at 225 nm for HCT, and at 232 nm for xipamide (XIP) added as an internal standard (IS). Separation of the three analyzed drugs and the IS was performed in less than 8 min. Migration times were 4.06, 5.23, 6.69, and 7.3 min for AML, HCT, BEN, and XIP, respectively. The findings proved that the proposed method was linear in the range of 10–80 μg/mL for all drugs with correlation coefficients >0.9994. The limit of detection (LOD) values of AML, HCT, and BEN were 1.004, 1.224, and 0.896 μg/mL, respectively, whereas the limit of quantification (LOQ) values were 3.124, 3.727, and 2.749 μg/mL for the cited drugs, respectively. Peak identity and purity were confirmed by DAD. The developed CZE method was applied for the analysis of the three antihypertensive drugs successfully in their combined pharmaceutical tablets, and it can be used for the quality control of single-pill combination (SPC) samples of these drugs in short time.

Słowa kluczowe

EN

capillary zone electrophoresis; benazepril; amplodipine besylate; hydrochlorothiazide

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2020
• Tom: Vol. 32, no. 4
• Strony: 219--227
• Opis fizyczny: Bibliogr. 44 poz., rys.

Twórcy

autor

Ahmed Hytham M.

• Menoufia University, Shebin El-Kom, Egypt

autor

Belal Tarek S.

• University of Alexandria, Alexandria 21521, Egypt

autor

Shaalan Rasha A.

• University of Alexandria, Alexandria 21521, Egypt
• Pharos University in Alexandria, Egypt

autor

Yazbi Fawzy A.

• University of Alexandria, Alexandria 21521, Egypt

autor

Elonsy Sohila M.

• Damanhour University, Damanhour, Egypt

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Uwagi

PL

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).