Microscopic analysis of the nanostructures impact on endothelial cells

• Autorzy: Kołodziejczyk Agnieszka Maria , Kucińska Magdalena , Jakubowska Aleksandra , Siatkowska Małgorzata , Sokołowska Paulina , Kotarba Sylwia , Makowski Krzysztof , Komorowski Piotr , Walkowiak Bogdan

Identyfikatory

DOI

10.34821/eng.biomat.154.2020.2-8

• Języki publikacji: EN

Abstrakty

EN

Nowadays nanostructures are more and more often designed as carriers for drug delivery, especially to improve the drug pharmacokinetics and pharmaco-dynamics. Numerous kinds of nanostructures are considered a good prospect for medical applications thanks to their small size, acceptable biocompatibility and toxicity. Due to the fact that nanotechnology is a new field of science, every nano-scale product must be thoroughly examined regarding its toxicity to the human body. This study provides new insights into effects of exposing endothelial cells to the selected nanostructures. Dendrimers of the fourth generation (PAMAMs), multi-walled carbon nanotubes (MWCNTs) and silver nanoparticles (SNPs) were used to evaluate nanostructures influence on endothelial cells in vitro. The nanostructures were evaluated via transmission electron microscopy and dynamic light scattering technique. The cells previously exposed to the nanostructures were observed and analyzed via the atomic force microscopy and scanning electron microscopy to obtain a quantitative evaluation of the cells morphology. The presence of multi-walled carbon nanotubes and silver nanoparticles on the cells surface was confirmed by the scanning electron microscopy. Our results confirm that the surface association and/or uptake of nanostructures by the cells resulting from physicochemical and biological processes, affect the cells morphology. Morphological changes can be induced by the membrane proteins interaction with nanomaterials, which trigger a sequence of intracel-lular biological processes.

Słowa kluczowe

EN

nanostructures; atomic force microscopy; transmission electron microscopy; scanning electron microscopy; cell morphology

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2020
• Tom: Vol. 23, no. 154
• Strony: 2--8
• Opis fizyczny: Bibliogr. 32 poz., tab., wykr., zdj.

Twórcy

autor

Kołodziejczyk Agnieszka Maria

• BioNanoPark Laboratories, 114/116 Dubois St., 93-465 Lodz, Poland

autor

Kucińska Magdalena

• BioNanoPark Laboratories, 114/116 Dubois St., 93-465 Lodz, Poland

autor

Jakubowska Aleksandra

• BioNanoPark Laboratories, 114/116 Dubois St., 93-465 Lodz, Poland

autor

Siatkowska Małgorzata

• BioNanoPark Laboratories, 114/116 Dubois St., 93-465 Lodz, Poland

autor

Sokołowska Paulina

• BioNanoPark Laboratories, 114/116 Dubois St., 93-465 Lodz, Poland
• Department of Pharmacology and Toxicology, Medical University of Lodz, Zeligowskiego St. 7/9, 90-752 Lodz, Poland

autor

Kotarba Sylwia

• BioNanoPark Laboratories, 114/116 Dubois St., 93-465 Lodz, Poland

autor

Makowski Krzysztof

• BioNanoPark Laboratories, 114/116 Dubois St., 93-465 Lodz, Poland
• Department of Biophysics, Institute of Materials Science, Lodz University of Technology, 1/15 Stefanowskiego St., 90-924 Lodz, Poland

autor

Komorowski Piotr

• BioNanoPark Laboratories, 114/116 Dubois St., 93-465 Lodz, Poland
• Department of Biophysics, Institute of Materials Science, Lodz University of Technology, 1/15 Stefanowskiego St., 90-924 Lodz, Poland

autor

Walkowiak Bogdan

• BioNanoPark Laboratories, 114/116 Dubois St., 93-465 Lodz, Poland
• Department of Biophysics, Institute of Materials Science, Lodz University of Technology, 1/15 Stefanowskiego St., 90-924 Lodz, Poland

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).