A new approach for determination of urinary 8-hydroxy-20-deoxyguanosine in cancer patients using reinforced solid/liquid phase microextraction combined with HPLC-DAD

• Autorzy: Al-Hashimi Nabil N. , Shahin Rand O. , El-Sheikh Amjad H. , Jibreel Malak J. , Alsakhen Nada A , Alqudah Abdelrahim M. , Oqal Muna K. , Abdelghani Jafar I.

Identyfikatory

DOI

10.1556/1326.2023.01142

• Języki publikacji: EN

Abstrakty

EN

The concentration level of urinary 8-hydroxy-20-deoxyguanosine (8-OHdG), an oxidative stress biomarker for various diseases especially cancer, has been attracted as a pathway suitable for diagnostic purposes. Determination of urinary 8-OHdG is challenging due to its low level within a complex matrix. In this study, a new approach of solid/liquid phase microextraction technique prior to high-performance liquid chromatography diode-array detection (HPLC-DAD) analysis was developed for the determination of trace levels of 8-OHdG in urine samples. The solid/liquid phase microextraction device was constructed by reinforcement of multi-walled carbon nanotubes into the pores of a short segment 2.5 cm of hollow fiber microtube with two ends heat sealed. Based on the optimized procedure, the selected analyte was extracted from an acidic sample solution (10 mL adjusted at pH 5 5) into the five extraction devices. After the extraction period (30 min), the 8-OHdG was eluted from the extraction device using methanol (350 μL) under ultrasonication for 5 min. The analytical performance of the method in synthetic urine samples showed good linearity (R2 > 0.999) with the limits of detection of 0.85 ng mL1 , and extraction recovery > 92.36%. The developed microextraction technique exhibited a confident sensitivity, feasible operation, and simplicity in comparison with other published methods and was valid to determinate trace 8-OHdG in urine cancer patients’ samples by using a cheap and commonly available HPLC-DAD instrument.

Słowa kluczowe

EN

cancer biomarker; HPLC-DAD; 8-hydroxy-20-deoxyguanosine; multi-walled carbon nanotubes; reinforcement solid phase microextraction; reinforcement liquid phase microextraction

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2024
• Tom: Vol. 36, no. 3
• Strony: 218--227
• Opis fizyczny: Bibliogr. 49 poz., rys., wykr.

Twórcy

autor

Al-Hashimi Nabil N.

• Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, The Hashemite University, P.O. Box 330127, Al-Zarqa 13133, Jordan

• https://orcid.org/0000-0002-1923-2405

autor

Shahin Rand O.

• Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, The Hashemite University, P.O. Box 330127, Al-Zarqa 13133, Jordan

• https://orcid.org/0000-0001-7330-4433

autor

El-Sheikh Amjad H.

• Department of Chemistry, Faculty of Science, The Hashemite University, P.O. Box 150459, Al-Zarqa 13115, Jordan

• https://orcid.org/0000-0001-8321-3236

autor

Jibreel Malak J.

• Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan

autor

Alsakhen Nada A

• Department of Chemistry, Faculty of Science, The Hashemite University, P.O. Box 150459, Al-Zarqa 13115, Jordan

• https://orcid.org/0000-0003-4543-4243

autor

Alqudah Abdelrahim M.

• Department of Clinical Pharmacy and Pharmacy Practice, Faculty of Pharmaceutical Sciences, The Hashemite University, P.O. Box 330127, Al-Zarqa 13133, Jordan

• https://orcid.org/0000-0003-3721-8225

autor

Oqal Muna K.

• Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, The Hashemite University, P.O. Box 330127, Al-Zarqa 13133, Jordan

• https://orcid.org/0000-0002-3766-5781

autor

Abdelghani Jafar I.

• Department of Chemistry, Faculty of Science, The Hashemite University, P.O. Box 150459, Al-Zarqa 13115, Jordan
• Department of Chemistry, Faculty of Science, The Hashemite University, P.O. Box 150459, Al-Zarqa 13115, Jordan

• https://orcid.org/0000-0001-7833-4746

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