Simultaneous quantification of salvianolic acid B and tanshinone IIA of salvia tropolone tablets by UPLC-MRM-MS/MS for pharmacokinetic studies

• Autorzy: Li J. R. , Li D. X. , Li L. , Deng W. L. , Ding L. S. , Xu H. X. , Zhou Y.
• Języki publikacji: EN

Abstrakty

EN

A rapid and sensitive ultraperformance liquid chromatography-multiple reaction monitoring-multi-stage/mass spectrometry (UPLC-MRM-MS/MS) method has been developed for simultaneous quantification of salvianolic acid B and tanshinone IIA of salvia tropolone tablets in dog plasma. This was achieved by performing quantification using the MRM acquisition with two channels of MRM-MS/MS and MS full scan for more accuracy qualitative results, and the fragmentation transitions of m/z 295→249, 191 for tanshinone IIA and m/z 297→279, 251 for IS in positive mode, m/z 717→519, 321 for salvianolic acid B and m/z 295→267, 239 for IS in negative mode were selected. The UPLC separation was achieved within 3 min in a single UPLC run. Linear calibration curves were obtained over the concentration range of 10 pg/mL^-1 ng/mL for tanshinone IIA and 100 pg/mL^-1 for salvianolic acid B. Lower limit of quantitation (LLOQ) was 10 pg/mL and 100 pg/mL for tanshinone IIA and salvianolic acid B, respectively. The inter-day and intra-day precision (relative standard deviation, RSD) in all samples were less than 8.21%, and the recoveries were over 85.9% for both tanshinone IIA and salvianolic acid B. The two channels of MRM with MS full scan approach could provide both qualitative and quantitative results without the need for repetitive analyses and resulted in the reduction of further confirmation experiments and analytical time. The pharmacokinetic study of the two active components of salvia tropolone tablets following oral gavage administration of dogs was thus explored with this method.

Słowa kluczowe

EN

salvia tropolone tablets; tanshinone IIA; salvianolic acid B; UPLC-MRM-MS/MS; pharmacokinetic study

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2014
• Tom: Vol. 26, no. 4
• Strony: 711--725
• Opis fizyczny: Bibliogr. 30 poz., rys., tab.

Twórcy

autor

Li J. R.

• Chinese Academy of Sciences Chengdu Institute of Biology Chengdu 610041 PR China

autor

Li D. X.

• Sichuan Academy of Chinese Medicine Sciences Sichuan Key Laboratory of Quality and Innovation Research of Chinese Materia Medica Chengdu 610041 PR China

autor

Li L.

• Waters China Limited Block 41, 1387 Zhangdong Road, Pudong New District Shanghai 201203 PR China

autor

Deng W. L.

• Sichuan Academy of Chinese Medicine Sciences Sichuan Key Laboratory of Quality and Innovation Research of Chinese Materia Medica Chengdu 610041 PR China

autor

Ding L. S.

• Chinese Academy of Sciences Chengdu Institute of Biology Chengdu 610041 PR China

autor

Xu H. X.

• Shanghai University of Traditional Chinese Medicines Shanghai 201203 PR China

autor

Zhou Y.

• Chinese Academy of Sciences Chengdu Institute of Biology Chengdu 610041 PR China

Bibliografia

• [1] X.S. Ouyang, K. Takahashi, K. Komatsu, et al., Jpn. J. Pharmacol., 87, 289 (2001)
• [2] J. Liu, H.M. Shen, and C.N. Ong, Cancer Lett., 153, 85 (2000)
• [3] X.Y. Ji, B.K.H. Tan, Y.C. Zhu, et al., Life Sci., 73, 1413 (2003)
• [4] Y.R. Lu, and L.Y. Foo, Phytochemistry, 59, 117 (2002)
• [5] C.B. Ai, and L.N. Li, J. Nat. Prod., 51, 145 (1988)
• [6] Y.H. Chen, S.J. Lin, H.H. Ku, et al., J. Cell. Biochem., 82, 512 (2001)
• [7] Y.L. Lin, C.H. Wu, M.H. Luo, et al., J. Ethnopharmacol., 105, 215 (2006)
• [8] I.S. Lay, C.C. Hsieh, J.H. Chiu, et al., J. Surg. Res., 115, 279 (2003)
• [9] Y.J. Wu, C.Y. Hong, S.J. Lin, et al., Arterioscl. Throm. Vas., 18, 481 (1998)
• [10] Y.L. Chen, C.S. Hu, F.Y. Lin, et al., J. Cell Biochem., 98, 618 (2006)
• [11] G.R. Zhao, H.M. Zhang, T.X. Ye, et al., Food Chem. Toxicol., 46, 73 (2008)
• [12] Z.Y. Fang, R. Lin, B.X. Yuan, et al., J. Ethnopharmacol. 115, 217 (2008)
• [13] G.Y. Zhou, W. Jiang, Y. Zhao, et al., Biochem. Pharmacol., 65, 51 (2003)
• [14] K. Takahashi, X.S. Ouyang, K. Komatsu, et al., Biochem. Pharmacol. 64, 745 (2002)
• [15] W.J. Xia, M. Yang, T.F. Fok, et al., Pediatr. Res., 58, 784 (2005)
• [16] X.J. Wang, Y.Q. Wei, S.L. Yuan, et al., Int. J. Cancer, 116, 799 (2005)
• [17] S.K. Kim, K.H. Jung, and B.C. Lee, Biol. Pharm. Bull., 32, 220 (2009)
• [18] B.Q. Lv, Y.C. Fan, and L.S. Sun, Tianjin Daxue Zhongyao Zazhi, 25, 32 (2006)
• [19] Y.F. Chen, I. Jaw, M.S. Shiao, et al., J. Chromatogr. A, 1088, 140 (2005)
• [20] X.R. Guo, X.H. Chen, L. Li, et al., J. Chromatogr. B, 873, 51 (2008)
• [21] D.E. Han, Z.D. Gao, D. Zhao, et al., Biomed. Chromatogr., 23, 1073 (2009)
• [22] H.P. Hao, G.J. Wang, Cui N, et al., Biomed. Chromatogr., 21, 1172 (2007)
• [23] E.J. Park, H.Y. Ji, N.J. Kim, et al., Biomed. Chromatogr., 22, 548 (2008)
• [24] X.L. Li, X.R. Li, L.J. Wang, et al., J. Pharm. Biomed. Anal., 44, 1106 (2007)
• [25] X.L. Wang, Z.R. Zhang, H.L. Fu, et al., Biomed. Chromatogr., 21, 1180 (2007)
• [26] Y. Liu, X.R. Li, Y.H. Li, et al., J. Pharm. Biomed. Anal., 53, 698 (2010)
• [27] Y.M. Ma, and T.M. Wang, Biomed. Chromatogr., 21, 217 (2007)
• [28] B.K. Matuszewski, M.L. Constanzer, and C.M. Chavez-Eng, Anal. Chem., 75, 3019 (2003)
• [29] Y. Zhan, J.P. Xu, J.B. Liang, L.S. Sheng, B.R. Xiang, Q.G. Zou, Z.J. Zhang, Chromatographia 68, 71 (2008)
• [30] D.Y. Gao, L.M. Han, L.H. Zhang, X.L. Fang, J.X. Wang, Arch. Pharm. Res., 32, 773 (2009)