Design of alginate microsphere formulation as a probiotics carrier

Łętocha, Anna; Michalczyk, Alicja; Miastkowska, Małgorzata; Sikora, Elżbieta

doi:10.24425/cpe.2023.146722

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Tytuł artykułu

Design of alginate microsphere formulation as a probiotics carrier

Autorzy

Łętocha Anna , Michalczyk Alicja , Miastkowska Małgorzata , Sikora Elżbieta

Treść / Zawartość

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Identyfikatory

DOI

10.24425/cpe.2023.146722

Warianty tytułu

Konferencja

24th Polish Conference of Chemical and Process Engineering, 13-16 June 2023, Szczecin, Poland. Guest editor: Prof. Rafał Rakoczy and 8th European Process Intensification Conference, 31.05–2.06.2023, Warsaw, Poland

Języki publikacji

Abstrakty

The process of obtaining alginate microspheres (AMs) by emulsification method was optimized by applying statistical analysis software. Ten batches of microspheres were prepared using the fractional plan 3K−p . AMs were obtained with two different methods: an ultrasonic homogenization (UH) process and a rotorstator mechanical homogenization (MH). The amount of a cross-linking agent (CaCl2), calcium chloride rate addition, and the sonication amplitude (UH) or the speed of rotor rotation (MH) were selected as formulation variables. All the batches were evaluated in terms of stability and size of the alginate microspheres. Approximation profiles were developed. As a result of the conducted research, stable alginate microspheres with sizes ranging from 10 to 30 micrometres were obtained. The obtained results showed that the quality of AMs was mainly affected by the concentration and the rate of calcium chloride addition into the system. Therefore, the role of calcium ions in the mechanisms of shell structuring was discussed. Lactobacillus casei bacteria were encapsulated into the batches found to be optimum. The high encapsulation efficiency (EE ) of the bacteria (72–94%) depending on the form) and their viability over time were obtained. The model developed in the study can be effectively utilized to achieve the AMs formulations.

Słowa kluczowe

alginate microspheres optimization Lactobacillus casei encapsulation emulsification

mikrosfery alginianowe optymalizacja enkapsulacja emulgowanie

Wydawca

Komitet Inżynierii Chemicznej i Procesowej Polskiej Akademii Nauk

Czasopismo

Chemical and Process Engineering : New Frontiers

Rocznik

2023

Tom

Vol. 44, nr 3

Strony

art. no. e20

Opis fizyczny

Bibliogr. 58 poz., rys., tab.

Twórcy

autor

Łętocha Anna

anna.letocha@doktorant.pk.edu.pl

Cracow University of Technology, Department of Chemical Engineering and Technology, Warszawska 24, 31-155 Cracow, Poland

autor

Michalczyk Alicja

Lukasiewicz Research Network – Institute of Industrial Organic Chemistry, Warsaw, Poland

https://orcid.org/0000-0002-9766-3227

autor

Miastkowska Małgorzata

Cracow University of Technology, Department of Chemical Engineering and Technology, Warszawska 24, 31-155 Cracow, Poland

https://orcid.org/0000-0002-4590-9184

autor

Sikora Elżbieta

Cracow University of Technology, Department of Chemical Engineering and Technology, Warszawska 24, 31-155 Cracow, Poland

https://orcid.org/0000-0003-4867-965X

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa nr POPUL/SP/0154/2024/02 w ramach programu "Społeczna odpowiedzialność nauki II" - moduł: Popularyzacja nauki (2025)

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Bibliografia

Identyfikator YADDA

bwmeta1.element.baztech-38822f31-c6fc-4878-9db7-fcae934c0695