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Tytuł artykułu

Investigation of functional diversity and activated sludge condition using Biolog® system

Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Activated sludge method dominates among the currently used biological wastewater treatment systems. The dynamic development of this technology implies need for research, most of which are carried out in laboratory conditions. Biolog system seems to be an effective tool that allows a quick assessment of microbial activity of activated sludge. The aim of this study was to verify Biolog® EcoPlates’ usage for comparing the functional and metabolic diversity of activated and excess sludge microbial communities, collected from WWTP “Klimzowiec” in Chorzów with the excess sludge subjected to the purification process, and then long-term adaptation in laboratory conditions. Changes in the functional diversity of activated sludge during the adaptation in the lab-scale were verified. It was found that the acclimatization process plays a crucial role in sludge adaptation to the laboratory environment. In addition, it has been shown that subjecting the excess sludge to purification may lead to changes in functional diversity of this community.
PL
Wśród współcześnie stosowanych systemów biologicznego oczyszczania ścieków dominuje metoda osadu czynnego. Dynamiczny rozwój tej technologii implikuje konieczność prowadzenia badań naukowych, które w większości wykonywane są w warunkach laboratoryjnych. System BIOLOG wydaje się być skutecznym narzędziem, pozwalającym na szybką ocenę stanu aktywności mikroorganizmów osadu czynnego. Przedmiotem badań było zastosowanie płytek Biolog® EcoPlate do porównania funkcjonalnej różnorodności i aktywności metabolicznej zespołów mikroorganizmów, występujących w osadzie nadmiernym i osadzie czynnym, pobranym z Oczyszczalni Ścieków „Klimzowiec” w Chorzowie oraz w osadzie nadmiernym, poddanym procesowi oczyszczania, a następnie 7-tygodniowej adaptacji w warunkach laboratoryjnych. Sprawdzono także zmiany różnorodności funkcjonalnej mikroorganizmów osadu czynnego pobranego z oczyszczalni ścieków podczas hodowli w warunkach laboratoryjnych. Stwierdzono, iż proces aklimatyzacji odgrywa ważną rolę w adaptowaniu osadu czynnego do warunków laboratoryjnych. Wykazano również, że poddanie osadu nadmiernego procesowi oczyszczania może prowadzić do zmian różnorodności funkcjonalnej tego środowiska.
Rocznik
Strony
119--126
Opis fizyczny
Bibliogr. 28 poz.
Twórcy
autor
  • Department of Biochemistry, Faculty of Biology and Environment Protection, University of Silesia, Jagiellońska 28, 40-032 Katowice, Poland
  • Department of Biochemistry, Faculty of Biology and Environment Protection, University of Silesia, Jagiellońska 28, 40-032 Katowice, Poland
autor
  • Department of Biochemistry, Faculty of Biology and Environment Protection, University of Silesia, Jagiellońska 28, 40-032 Katowice, Poland
Bibliografia
  • [1] Amman R., Lemmer H. Wagner M.; Monitoring the community structure of wastewater treatment plants: a comparison of old and new techniques. FEMS Microbiology Ecology, 25, 1998; p.205-215.
  • [2] Keakewipat K., Grady Jr. C.P.L.; Microbial population dynamics in labolatory-scale activated sludge reactors. Water Science and Technology, 46, 2002; p.19-27.
  • [3] Al-Mutairi N.Z.; Variable distributional characteristics of substrate utilization patterns in activated sludge plants in Kuwait Bioresource Technology, 100, 2009; p.1524-1532.
  • [4] Yang C., Zhang W., Liu R., Li Q., Li B., Wang S., Song C., Qiao C., Mulchandani A.; Phylogenetic diversity phylogenetic diversity and metabolic potential of activated sludge microbial communities in full-scale wastewater treatment plants. Environmental Science and Technology, 45, 2011; p.7408-7415.
  • [5] Barbusinski K.; Adaptation of activated sludge to laboratory research conditions. Environmental Protection Engineering, 17, 1991; p.57-65.
  • [6] van der Gast C.J., Whiteley A.S., Thompson I.P.; Temporal dynamics and degradation activity of an bacterial inoculum for treating waste metal-working fluid. Environmental Microbiology, 6(3), 2004; p.254-263.
  • [7] Forney L. J., Liu W. T., Guckert J. B., Kumagai Y., Namkung E., Nishihara T., Larson R. J.; Structure of microbial communities in activated sludge: potential implications for assessing the biodegradability of chemicals. Ecotoxicology and Environmental Safety, 49, 2001; p.40-53.
  • [8] Kim T.S, Jeong J.Y, Wells G.F., Park H.D.; General and rare bacterial taxa demonstrating different temporal dynamic patterns in an activated sludge bioreactor. Applied Microbiology and Biotechnology, 97, 2013; p.1755-1765.
  • [9] Jenkins D., Richard M. G., Daigger G. T.; Manual on the causes and control of activated sludge bulking and foaming. Lewis Publishers, Michigan, USA, 1993.
  • [10] Kaiser S., Guckert J., Gledhill D.; Comparison of activated sludge microbial communities using BiologTM microplates. Water Science and Technology, 37(4-5), 1998; p.57-63.
  • [11] Garland J. L., Mills A. L.; Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbonsource utilization. Applied and Environmental Microbiology, 57(8), 1991; p.2351-2359.
  • [12] Insam H., Rangger A.; Microbial communities: functional versus structural approaches. Springer-Verlag, Berlin, Germany, 1997.
  • [13] Preston-Mafham J., Boddy L., Randerson P. F.;Analysis of microbial community functional diversity using sole-carbon-source utilisation profiles – a critique. FEMS Microbiology Ecology, 42, 2002; p.1-14.
  • [14] Papadimitriou CH., Palaska G., Lazaridou M., Samaras P., Sakellaropoulos, G.P.; The effects of toxic substances on the activated sludge microfauna. Desalination, 211, 2007; p.177-191.
  • [15] American Public Health Association, American Water Works Association, and Water Environment Federation; Standard Methods for the Examination of Water and Wastewater, 20th edition. American Public Health Association, Washington, D.C., 1998.
  • [16] Garland J.L.; Analysis and interpretation of community- level physiological profiles in microbial ecology. FEMS Microbiology Ecology, 24, 1997; p.289-300.
  • [17] Allen M. J., Edberg S. C., Reasoner D., J.; Heterotrophic plate count bacteria-what is their significance in drinking water? International Journal of Food Microbiology, 92(3), 2004; p.265-274.
  • [18] Wagner M., Loy A.; Bacterial community composition and function in sewage treatment systems. Current Opinion in Biotechnology, 13, 2002; p.218-227.
  • [19] van der Broeck R.M.R., van Impe J.F.M., Smets I.Y.M.; Assessment of activated sludge stability in lab-scale experiments. Journal of Biotechnology, 141, 2009; p.147-154.
  • [20] Zawani Z., Chuah-Abdullah L., Ahmadun F.R., Abdan K.; Acclimatization process of microorganisms from activated sludge in Kenaf-Retting Wastewater. Developments in Sustainable Chemical and Bioprocess Technology, 2013; p.59-64.
  • [21] Urbain V., Pys E., Block J.C., Manem J.; Composition and activity of activated sludge under starvation conditions. Environmental Technology, 14, 1993; p. [22] Chetty M., Mudaly D.D., Bux F.; Manipulation of activated sludge for the direct detection of polyphosphate accumulating bacteria. WISA 2000 BiennialConference, Sun City, South Africa, 2000.
  • [23] Mendrycka M., Stawarz M.; Possibilities of applications of biocomponent for biological processes of tanning sewage by activated sludge-aided method. Inżynieria Ekologicza, 28, 2012; p.43-56.
  • [24] Babko R., Kuzmina T., Jaromin-Gleń K., Bieganowski A.; Bioindication assessment of activated sludge adaptation in a lab-scale experiment. Ecological Chemistry and Engineering, 21(4), 2014; p.605-616.
  • [25] Muszyński A., Załęska-Radziwiłł M.; Polyphosphate accumulating organisms in treatment plants with different wastewater composition. Architecture Civil Engineering Environment, 4, 2015; p.605-616.
  • [26] Webber K.P., Legge R.L.; One-dimensional metric for Fu Methods 79, 2009; p.55-61731-740.
  • [27] Wilson W.A., Roach P.J., Montenero M., Baroja- Fernández E., Muñoz J., Eydallin G., Viale A.M., Pozueta-Romero J.; Regulation of glycogen metabolism in yeast and bacteria. FEMS Microbiology Reviews, 34(6), 2010; p.952-985.
  • [28] Palm J., Jenkins D.; Relationship between organic loading, dissolved oxygen concentration and sludge settleability in the completely mixed activated sludge process. Journal of Water Pollution Control Federation, 52(10),1980; p.2484-2506.
Uwagi
PL
Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-374e1485-a546-463d-8105-2cc2d8e9e1c5
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