Contracaecum spp. from endemic Baikal fishes: the Baikal yellowfin Cottocomephorus grewingkii (Dybowski, 1874) and the longfin Baikal sculpin Cottocomephorus inermis (Yakovlev, 1890)

• Autorzy: Rusinek O. , Kulikowski M. , Najda K. , Rokicki J.

Identyfikatory

DOI

10.1515/ohs-2015-0007

• Języki publikacji: EN

Abstrakty

EN

All the nematodes found in body cavities of the examined endemic Baikal fishes: 88 Baikal yellowfin Cottocomephorus grewingkii (Dybowski, 1874) and 35 longfin Baikal sculpin Cottocomephorus inermis (Yakovlev, 1890) were identified as Contracaecum osculatum baicalensis (Mozgovoi and Ryjikov, 1950) L3 larvae. The prevalence, mean intensity, intensity range and abundance of the nematodes in C. grewingkii were 37.5%, 2.55, 1-31, and 0.96, respectively, the corresponding values in C. inermis were 60.0%, 2.43, 1-10, and 1.46. The infestation level in C. grewingkii was significantly higher than in C. inermis (Mann-Whitney U-test, p<0.02). The number of parasites was found to increase with the fish length. Although in both and C. inermis, the anisakids were more frequent in males (prevalence of 52.17 and 67.76%, respectively) than in females (prevalence of 35.39 and 42.86%, respectively), differences between the sexes in the infestation level in the two species were not significant (Mann-Whitney U-test, P= 0.09 and P=0.23, respectively). The molecular method applied (PCF-RFLP) allows to identify all the nematodes in both examined fish species as C. osculatum baicalensis.

Słowa kluczowe

EN

Contracaecum osculatum baicalensis; Anisakidae; Cottocomephorus; PCR-RFLP; molecular method

• Wydawca: Institute of Oceanography University of Gdańsk
• Czasopismo: Oceanological and Hydrobiological Studies
• Rocznik: 2015
• Tom: Vol. 44, No. 1
• Strony: 68--73
• Opis fizyczny: Bibliogr. 27 poz., tab.

Twórcy

autor

Rusinek O.

• Baikal Museum of Irkutsk Scientific Center of Siberian Branch of the Russian Academy of Sciences, Akademicheskaya str.,1, 664520 Listvyanka, Russia

autor

Kulikowski M.

• Department of Invertebrate Zoology and Parasitology, University of Gdańsk, ul. Wita Stwosza 59, 80-308 Gdańsk, Poland

autor

Najda K.

• Department of Invertebrate Zoology and Parasitology, University of Gdańsk, ul. Wita Stwosza 59, 80-308 Gdańsk, Poland

autor

Rokicki J.

• Department of Invertebrate Zoology and Parasitology, University of Gdańsk, ul. Wita Stwosza 59, 80-308 Gdańsk, Poland

Bibliografia

• [1]. Bauer, O.N. (1987). Key to the parasites of freshwater fish of the USSR. Metazoan parasites. Leningrad: Izdatel’stvo Nauka (in Russian).
• [2]. D’Amelio, S., Mattiucci, S., Paggi, L., Koie, L., Podvyaznaya, I.,Pugachev, O., Rusinek, O., Timoshkin, O. & Nascetti, G. (1995). Taxonomic rank and origin of Contracaecum osculatum baicalensis Mozgovoi and Ryjikov, 1950, parasite of Phoca sibirica from Lake Baikal, with data on its occurrence in fish hosts. Abstracts of 4th International Symposium of Fish Parasitology, Munich October 3-7, 27.
• [3]. Dogel’, V.A., Bogolepova, I.I. & Smirnova, K.V. (1949). Parazitofauna Bajkala i eё zoogeografičeskoe značenie, Vestnik Leningradskogo Universiteta 7: 13-34 (in Russian).
• [4]. Dzido, J. (2011). Identyfikacja molekularna oraz zróżnicowanie wewnątrzgatunkowe pasożytniczych nicieni z rodziny Anisakidae. Unpublished doctoral dissertation, University of Gdańsk, Gdańsk, Poland.
• [5]. Dzyuba, E.V (2004). Issledowanie piščevyh strategii pelagičeskich ryb Bajkala. Unpublished doctoral dissertation, Institut Biologii Vnutrennyh Vod im. I.D. Papanina RAN, Borok, Russia (in Russian).
• [6]. Dzyuba, E.V, Mel’nik, N.G. & Naumova, E.Y. (2000). Feeding spectra of the young of Cottocomephorus inermis (Cottidae) in Lake Baikal. J. Ichthyol. 40: 342-345.
• [7]. Hoarau, G., Holla, S., Lescasse, R., Stam, W.T. & Olsen, J.L. (2002). Heteroplasmy and evidence for recombination in the mitochondrial control region of the flatfish Platichthys flesus. Mol. Biol. Evol. 19(12): 2261-2264.
• [8]. Kijewska, A., Rokicki, J., Sitko, J. & Węgrzyn, G. (2002). Ascaridoidea: A simple DNA assay for identification of Contracaecum osculatum baicalensis from Baikal Cottocomephorus spp.11 species infecting marine and freshwater fish, mammals, and fish-eating birds. Exp. Parasitol. 101(1): 35-39. DOI: 10.1016/S0014-4894(02)00031-0.
• [9]. Koie, M. & Fagerholm, H. P. (1995). The life cycle of Contracaecum osculatum (Rudolphi, 1802) sensu stricto (Nematoda, Ascaridoidea, Anisakidae) in view of experimental infections. Parasitol. Res. 81: 481-489.
• [10]. Kozhov, M.M. (1963). Lake Baikal and its Life. Monogr. Biol. 11, The Hague: Junk Publishers.
• [11]. Lâjman, E.M. (1933). Parazitičeskie červi ozera Bajkal. Trudy Bajkalskoj Limnologičeskoj Stancji 4: 5-98 (in Russian).
• [12]. Margolis, L., Esch, G. W., Holmes, J. C., Kuris, A. M. & Schad G. A. (1982). The use of ecological terms in parasitology (report of an ad hoc committee of the American Society of Parasitologists), J. Parasitol. 68(1): 131 - 133.
• [13]. Mattiucci, S., Nascetti, G., Cianchi, R., Paggi, L., Arduino, P., Margolis, L., Brattey, J., Webb, S., D’Amelio, S., Orecchia, P. & Bullini, L. (1997). Genetic and ecological data on the Anisakis simplex complex, with evidence for a new species (Nematoda, Ascaridoidea, Anisakidae). J. Parasitol. 83(3): 401-416.
• [14]. Mattiucci, S. & Nascetti, G. (2007). Genetic diversity and infection levels of anisakid nematodes parasitic in fish and marine mammals from Boreal and Austral hemispheres. Vet. Parasitol. 148: 43-57. DOI: 10.1016/j.vetpar.2007.05.009.
• [15]. Mattiucci, S. & Nascetti, G. (2008). Advances and trends in the molecular systematics of anisakid nematodes, with implications for their evolutionary ecology and host - parasite co - evolutionary processes. Adv. Parasitol. 66: 48 - 148. DOI: 10.1016/S0065-308X(08)00202-9.
• [16]. Mazepova, G.F. (1998). The role of copepods in the Baikal ecosystem. J. Mar. Syst. 15: 113-120. DOI: 10.1016/S0924- 7963(97)00065-1.
• [17]. Melnik, N.G., Timoshkin, O.A. & Sideleva, V.G. (1995). Distribution of M. branickii and some characteristics of its ecology. Guide and key to pelagic animals of Baikal. Novosibirsk: Izdatel’stvo Nauka.
• [18]. Rolbiecki, L. (2002). A rapid method for preparing semipermanent glycerol-jelly parasite Mount. Wiadomości Parazytologiczne 48: 87-88.
• [19]. Rudstam, L.G., Melnik, N.G., Timoshkin, O.A., Hansson, S., Pushkin, S.V & Nemov, V. (1992). Diel Dynamics of an Aggregation of Macrohectopus Branickii (DYB.) (Amphipoda, Gammaridae) in the Barguzin Bay, Lake Baikal, Russia. J. Gt. Lakes Res. 18(2): 286-297. DOI 10.1016/S0380-1330(92)71296-9.
• [20]. Rusinek, O.T. (2007). Fish parasites of Lake Baikal (fauna, communities, zoogeography and historical background). Moscow: KMK Scientific Press Ltd. (in Russian).
• [21]. Sambrook, J., Fritsh, E.F. & Maniatis, T. (1989). Molecular cloning: A laboratory manual. New York: Cold Spring Harbor Laboratory Press.
• [22]. Sudarikov, V.E. & Ryzhikov, K.M. (1951). K biologii Contracaecum osculatum baicalensis - nematody bajkalskoj nerpy. Trudy Gel’mintologiceskoj Laboratorii 55: 59-66, (in Russian).
• [23]. Valtonen, E.T., Fagerholm, H.P. & Helle, E. (1988). Contracaecum osculatum (Nematoda: Anisakidae) in fish and seals from Bothnian Bay (Northeastern Baltic Sea). Int. J. Parasitol. 18(3): 365-370. DOI: 10.1016/0020- 7519(88)90146-4.
• [24]. Zaika V.E. (1965). Parazitofauna ryb ozera Bajkal. Russia: Izdatel’stvo Nauka, (in Russian).
• [25]. Zhu, X., D’Amelio, S., Paggi, L. & Gasser, R.B. (2000). Assessing sequence variation in internal transcribed spacers of ribosomal DNA within and among members of the Contracaecum osculatum complex (Nematoda: Ascaridoidea: Anisakidae). Parasitol. Res. 86(8): 677 - 683. DOI: 10.1007/PL00008551.
• [26]. Zhu, X., Gasser, R.B., Podolska, M. & Chilton, N.B. (1998). Characterization of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences. Int. J. Parasitol. 28(12): 1911 - 1921. DOI: 10.1016/S0020- 7519(98)00150-7.
• [27]. Zubin, A.A. (1992). Diet of benthopelagic Baikal sculpins (Scorpaeniformes, Cottoidei), J. Ichthyol. 32: 42-47.