In vitro evaluation of poly(L-lactide-co-glycolide) membrane

• Autorzy: Krok M. , Hess R. , Wojak I. , Kościelniak D. , Scharnweber D. , Pamuła E.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to prepare and evaluate the properties of a new membrane dedicated for the treatment of bone defects in periodontology according to guided tissue regeneration (GTR) technique. The first part of this study was to prepare the membrane from resorbable poly(L-lactide-co-glycolide) (PLGA) and verify its microstructure. Biological evaluation was lead using the cells interesting from the point of view of GTR, e.g. human fibroblasts and mesenchymal stem cells (hMSC). It was found that the obtained membrane has asymmetric microstructure and defined pore size. Cell culture experiments show that the membrane is biocompatible with fibroblasts and hMSC. Both types of cell proliferated well on the membrane. HMSC cultured on the membrane exhibited better osteogenic differentiation and higher mineralization as compared to control tissue culture polystyrene.

Słowa kluczowe

EN

in vitro; membranes; bone defects

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2010
• Tom: Vol. 13, no. 94
• Strony: 7--10
• Opis fizyczny: Bibliogr. 12 poz., wykr., zdj.

Twórcy

autor

Krok M.

• AGH- University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biomaterials, al. Mickiewicza 30, 30-059 Krakow, Poland

autor

Hess R.

• Technische Universität Dresden, Max Bergmann Center of Biomaterials, Budapester Str. 27, 01069 Dresden, Germany

autor

Wojak I.

• AGH- University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biomaterials, al. Mickiewicza 30, 30-059 Krakow, Poland

autor

Kościelniak D.

• Jagiellonian University, Collage of Medicine, Department of Pedodontics, ul. Montelupich 4, 31-155 Krakow, Poland

autor

Scharnweber D.

• Technische Universität Dresden, Max Bergmann Center of Biomaterials, Budapester Str. 27, 01069 Dresden, Germany

autor

Pamuła E.

• AGH- University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biomaterials, al. Mickiewicza 30, 30-059 Krakow, Poland

Bibliografia

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• [3] Taguchi Y., Amizuka N., Nakadate M., Ohnishi H., Furii N., Oda K., Nomura S., Maeda T.: A histological evaluation for guided bone regeneration induced by a collagenous membrane, Biomaterials 26 (2005), 6158-6166.
• [4] Alpar B., Leyhausen G., Günay H., Geurtsen W.: Compatybility of resorbable and nonresorbable guided tissue regeneration membranes in cultures of primary human periodontal ligament fibroblasts and human osteoblast-like cells, Clin Oral Invest 4 (2000), 219-225.
• [5] Yang F., Both S. K., Yang X., Walboomers X. F., Jansen J. A.: Development of an electrospun nano-apatite/PCL composite membrane for GTR/GBR application, Acta Biomaterialia 5 (2009), 3295-3304.
• [6] Kikuchi M., Koyama Y., Yamada T., Imamura Y., Okada T., Shirahama N., Akita K., Takakuda K., Tanaka J.: Development of guided bone regeneration membrane composed of tricalcium phosphate and poly(L-lactide-co-glicolide-co-e-caprolactone) composites, Biomaterials 25 (2004), 5979-5986
• [7] Stavropoulos A., Sculean A., Karring T.: GTR treatment of intrabony defects with PLA/PGA copolymer or collagen bioresorbable membranes in combinationwith deproteinized bovine bone (Bio-Oss), Clin Oral Invest 8 (2004), 226-232.
• [8] Nieminen T., Kallela I., Keränen J., Hiidenheimo I., Kainulainen H., Wuolijoki E., Rantala I.: In vivo and in vitro degradation of a novel bioactive guided tissue regeneration membrane, Int J Oral Maxillofac Surg 35 (2006), 727-732.
• [9] Owen G. Rh., Jackson J., Chehroudi B., Burt H., Brunette D. M.: A PLGA membrane controlling cell behaviour for promoting tissue regeneration, Biomaterials 26 (2005), 7477-7456.
• [10] Xianmiao C., Yubao L., Yi Z., Li Z., Jidong L., Huanan W.: Propertis nad in vitro biological evaluation of nano-hydroxyapatite/chitosan membranes for bone guided regeneration, Mat Sci Eng C 29 (2009), 29-35.
• [11] Ho M-H., Hsieh C-C., Hsiao S-W., Thien D. V. H.: Fabrication os asymmetric chitosan GTR membranes for the treatment of periodontal disease, Carbohydrate Polym 79 (2010), 955-963.
• [12] P-391519. Method of manufacturing of resorbable membrane for guided tissue regeneration / Sposób wytwarzania resorbowalnej membrany do sterowanej regeneracji tkanek inventors / inventors / wynalazcy: E. Pamuła, S. Błażewicz, M. Krok, D. Kościelniak, P. Dobrzyński. (2010-06-15).