Preliminary studies of interaction between nanotubes and toll-like receptors

• Autorzy: Mozolewska M. A. , Krupa P. , Rasulev B. , Liwo A. , Leszczyński J.
• Języki publikacji: EN

Abstrakty

EN

Toll-like receptors ( TLR s) are a group of proteins which play a crucial role in the innate immune system. The main function of TLR s is to recognize structurally conserved molecules, which are inserted to the organism of the host by microbes, and then to activate the immune response. Current development of drugs is often connected not only with the drug itself, but also with the way it is delivered into the human body to interact direc tly with the source of the problem. Carbon nanostructures, particularly nanotubes, are one of the car rier molecules of the future. However, there is still no knowledge about the exact mechani sms of toxicity and possible interactions with macromolecules, such as proteins. In our study we tr ied to determine, if the nanotubes could interfere with the innate immune system by interac ting with TLR s. For this purpose, we used the following TLR structures downloaded from the RCSB Protein Data Bank: TLR 2 (3 A 7 C ), TLR 4/ MD (3 FXI ), TLR 5 (3 V 47), TLR 3 (2 A 0 Z ), and the complexes of TLR 1/ TLR 2 (2 Z 7 X ) and TLR 2/ TLR 6 (3 A 79). The preliminary results of our Steered Molecular Dynamics ( SMD ) simulations have shown that nanotubes interact very strongly with the binding pockets of some receptors ( e.g. TLR 2), which results in their binding to these sites without subst antial use of the external force.

Słowa kluczowe

EN

CNTs; toll-like receptors; molecular dynamics

PL

CNTs; receptory toll-like; dynamika molekularna

• Wydawca: Politechnika Gdańska
• Czasopismo: TASK Quarterly : scientific bulletin of Academic Computer Centre in Gdansk
• Rocznik: 2014
• Tom: Vol. 18, No 4
• Strony: 351--355
• Opis fizyczny: Bibliogr. 7 poz., rys., wykr.

Twórcy

autor

Mozolewska M. A.

• Faculty of Chemistry, University of Gdansk Wita Stwosza 63, 80-952 Gdansk, Poland

autor

Krupa P.

• Faculty of Chemistry, University of Gdansk Wita Stwosza 63, 80-952 Gdansk, Poland

autor

Rasulev B.

• Interdisciplinary Center for Nanotoxicity Department of Chemistry and Biochemistry, Jackson State University 1325 J. R. Lynch St. 17910, 39217 Jackson, MS, USA

autor

Liwo A.

• Faculty of Chemistry, University of Gdansk Wita Stwosza 63, 80-952 Gdansk, Poland

autor

Leszczyński J.

• Interdisciplinary Center for Nanotoxicity Department of Chemistry and Biochemistry, Jackson State University 1325 J. R. Lynch St. 17910, 39217 Jackson, MS, USA

Bibliografia

• [1] Christmas P 2010 Nat. Educ. 3 (9) 85
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• [3] Kolosnjaj J, Szwarc H and Moussa F 2007 Adv. Exp. Med. Biol. 620 181
• [4] Popov V N 2004 Mater. Sci. Eng. 43 61
• [5] Case K M D A, Darden T A, Cheatham T E III, Simmerling C L, Wang J, Duke R E, Walker R L and Zhang W 2012, AMBER 12, University of California, San Francisco
• [6] Schmidt M W, Baldridge K K, Boatz J A, Elbert S T, Gordon M S, Jensen J H, Koseki S, Matsunaga N, Nguyen K A, Su S, Windus T L, Dupuis M and Montgomery J A 1993 J. Comput. Chem. 14 (11) 1347
• [7] Dupradeau F-Y, Pigache A, Zaffran T, Savineau C, Lelong R, Grivel N, Lelong D, Rosanski W and Cieplak P 2010 Phys. Chem. Chem. Phys. 12 (28) 7821