Simultaneous determination of seven sesquiterpene lactone glucosides in Ixeris dentata by high-performance liquid chromatography coupled with tandem mass spectrometry and their antiviral activities

• Autorzy: Park SeonJu , Kim Nanyoung , Park Jun Hyung , Lee Sang-Won , Song Jae-Hyoung , Ko Hyun-Jeong , Chae Han-Jung , Kim Hyung-Ryong , Kim Seung Hyun
• Języki publikacji: EN

Abstrakty

EN

Ixeris dentata (Thunb. ex Thunb.) Nakai (Asteraceae), a well-known edible vegetable in Asia, contains various bioactive secondary metabolites, including sesquiterpene lactones. In this study, a high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) method has been developed and validated for simultaneous determination of seven sesquiterpene lactone glucosides isolated from the roots of I. dentata. In addition, these compounds were evaluated in terms of their antiviral activities against coxsackievirus B3 (CVB3) and human enterovirus 71 (EV71). The developed method was validated in terms of linearity (R2 > 0.9996), precision (RSD < 2.24%), accuracy (96.30–102.77%), and stability (RSD < 1.94%) and successfully applied to the quantitation of the I. dentata root samples collected from six different regions of Korea. The content of sesquiterpene lactone glucosides varied significantly based on the region. For the antiviral activities, guaianolides with an ester group at C-8 (compounds 6 and 7) showed the most potent activities against CBV3, while germacranolide (compound 5) showed the most consistent antiviral activity against both CVB3 and EV71. The method was validated to be simple and reliable to simultaneously determine seven putative bioactive sesquiterpene lactone glucosides, the substantial chemotaxonomic markers, in I. dentata root samples

Słowa kluczowe

EN

Ixeris dentata; guaianolide; germacranolide; HPLC-MS/MS; antiviral activity

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2019
• Tom: Vol. 31, no. 4
• Strony: 280--285
• Opis fizyczny: Bibliogr. 24 poz., rys., tab.

Twórcy

autor

Park SeonJu

• College of Pharmacy, Yonsei Institute of Pharmaceutical Science, Yonsei University, Incheon, Korea

autor

Kim Nanyoung

• College of Pharmacy, Yonsei Institute of Pharmaceutical Science, Yonsei University, Incheon, Korea

autor

Park Jun Hyung

• College of Pharmacy, Yonsei Institute of Pharmaceutical Science, Yonsei University, Incheon, Korea

autor

Lee Sang-Won

• Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Chungbuk, Korea

autor

Song Jae-Hyoung

• Laboratory of Microbiology and Immunology, College of Pharmacy, Kangwon National University, Chuncheon, Korea

autor

Ko Hyun-Jeong

• Laboratory of Microbiology and Immunology, College of Pharmacy, Kangwon National University, Chuncheon, Korea

autor

Chae Han-Jung

• Department of Pharmacology and Institute of Cardiovascular Research, Sechool of Medicine, Chonbuk National University, Chonbuk, Korea

autor

Kim Hyung-Ryong

• Graduate School, DGIST, Daegu, Korea

autor

Kim Seung Hyun

• College of Pharmacy, Yonsei Institute of Pharmaceutical Science, Yonsei University, Incheon, Korea

Bibliografia

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Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).