Atrazine toxicity in marine algae Chlorella vulgaris, in E. coli lux and gfp biosensor tests

Matejczyk, Marzena; Kondzior, Paweł; Ofman, Piotr; Juszczuk-Kubiak, Edyta; Świsłocka, Renata; Łaska, Grażyna; Wiater, Józefa; Lewandowski, Włodzimierz

doi:10.24425/aep.2023.147331

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Tytuł artykułu

Atrazine toxicity in marine algae Chlorella vulgaris, in E. coli lux and gfp biosensor tests

Autorzy

Matejczyk Marzena , Kondzior Paweł , Ofman Piotr , Juszczuk-Kubiak Edyta , Świsłocka Renata , Łaska Grażyna , Wiater Józefa , Lewandowski Włodzimierz

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DOI

10.24425/aep.2023.147331

Warianty tytułu

Toksyczność atrazyny w glonach Chlorella vulgaris oraz w biosensorowych testach E. coli z genami gfp i lux

Języki publikacji

Abstrakty

Atrazine (ATR) is a widely used chlorinated herbicide from the s-triazine group. Due to the widespread use of ATR, it leaks into the environment and is detected in drinking water, exceeding the WHO-acceptable concentration of atrazine in drinking water, which is 2 μg/L. The aim of our study was to determine toxicity, protein degradation and genotoxicity of ATR at concentrations of 10; 1; 0.1; 0.01 mg/L on Chlorella vulgaris and with the application of E. coli bioluminescent biosensor strains. We measured the content of chlorophyll a, b, carotenoids in Chlorella vulgaris and the inhibition of this algae culture growth. E. coli RFM443 strains with gene constructs grpE:luxCDABE, lac:luxCDABE, recA:luxCDABE and E. coli strain MM294 trc:luxCDABE were used to determine toxicity, degradation of cellular proteins and genotoxicity. On the base of the obtained results, we concluded that ATR in the tested concentrations shows a toxic effect in relation to Chlorella vulgaris. ATR is toxic and genotoxic in E. coli RFM443 strains with grpE, lac, recA promoters and causes degradation of cellular proteins. Moreover, we have detected ATR toxicity toward the GFP protein in E. coli strain MM294-GFP. Taking into account the toxicity and genotoxicity of ATR documented in our research and in the experiments of other authors, we conclude that the presence of this herbicide in surface waters and drinking water is a serious threat to living organisms.

Atrazyna (ATR) to szeroko stosowany na całym świecie chlorowany herbicyd z grupy s-triazyn. Ze względu na powszechne stosowanie, ATR przedostaje się do środowiska i jest wykrywana w wodzie pitnej, przekraczając dopuszczalne przez WHO stężenie, które wynosi 2 μg/L. W przedstawionych badaniach określono toksyczność ATR w stężeniach 10; 1; 0.1; 0.01 mg/L na glonach Chlorella vulgaris oraz z zastosowaniem E. coli mikrobiologicznych biosensorów z genami reporterowymi gfp i lux. Toksyczność oszacowano na podstawie zawartości chlorofilu a, b, karotenoidów w Chlorella vulgaris oraz zahamowania wzrostu tej kultury alg. Szczepy E. coli RFM443 z konstruktami genowymi grpE:luxCDABE, lac:luxCDABE, recA:luxCDABE i szczep E. coli MM294 trc:luxCDABE wykorzystano do określenia toksyczności, degradacji białek komórkowych i genotoksyczności. W przeprowadzonych badaniach wykryto, że ATR w analizowanych stężeniach wykazuje działanie toksyczne w stosunku do Chlorella vulgaris. W przypadku ATR stwierdzono właściwości toksyczne i genotoksyczne oraz potencjał degradacji białek w szczepach E. coli RFM443 z promotorami grpE, lac, recA. Ponadto wykryto toksyczność ATR w stosunku do białka GFP w szczepie E. coli MM294-GFP. Biorąc pod uwagę udokumentowaną w badaniach własnych oraz w doświadczeniach innych naukowców toksyczność i genotoksyczność ATR, obecność tego herbicydu w wodach powierzchniowych i wodzie pitnej stanowi poważne zagrożenie dla organizmów żywych.

Słowa kluczowe

ecotoxicology atrazine Chlorella vulgaris bioluminescent biosensors water contamination

ekotoksykologia atrazyna chlorella vulgaris czujniki bioluminescencji zanieczyszczenie wody

Wydawca

Institute of Environmental Engineering, Polish Academy of Sciences

Czasopismo

Archives of Environmental Protection

Rocznik

2023

Tom

Vol. 49, no 3

Strony

87--99

Opis fizyczny

Bibliogr. 41 poz., rys., tab., wykr.

Twórcy

autor

Matejczyk Marzena

m.matejczyk@pb.edu.pl

Bialystok University of Technology, Faculty of Civil Engineering and Environmental Sciences, Department of Chemistry, Biology and Biotechnology, Bialystok, Poland

https://orcid.org/0000-0001-9978-2273

autor

Kondzior Paweł

Bialystok University of Technology, Faculty of Civil Engineering and Environmental Sciences, Department of Chemistry, Biology and Biotechnology, Bialystok, Poland

autor

Ofman Piotr

Bialystok University of Technology, Department of Environmental Engineering Technology, Bialystok, Poland

autor

Juszczuk-Kubiak Edyta

Institute of Agricultural and Food Biotechnology-State Research Institute, Laboratory of Biotechnology and Molecular Engineering, Warsaw, Poland

autor

Świsłocka Renata

Bialystok University of Technology, Faculty of Civil Engineering and Environmental Sciences, Department of Chemistry, Biology and Biotechnology, Bialystok, Poland

autor

Łaska Grażyna

Department of Agri-Food Engineering and Environmental Management, Bialystok University of Technology, Bialystok, Poland

autor

Wiater Józefa

Bialystok University of Technology, Department of Agricultural and Food Engineering and Environmental Management, Bialystok, Poland

autor

Lewandowski Włodzimierz

Bialystok University of Technology, Faculty of Civil Engineering and Environmental Sciences, Department of Chemistry, Biology and Biotechnology, Bialystok, Poland

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Uwagi

Opracowane ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).

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Bibliografia

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bwmeta1.element.baztech-2e0065e9-a9e8-4585-84f3-f2cb14f290b5