Simultaneous analysis of six bioactive lignans in Phyllanthus species by reversed phase hyphenated high performance liquid chromatographic technique

• Autorzy: Shanker K. , Singh M. , Srivastava V. , Verma R. K. , Gupta A. K. , Gupta M. M.
• Języki publikacji: EN

Abstrakty

EN

An online-hyphenated high-performance liquid chromatography-photodiode array-mass spectrometry (HPLC-PDA-MS) analytical method was developed for the simultaneous determination of six lignans of therapeutic importance in four Phyllanthus spp. (P. amarus, P. maderaspatensis, P. urinaria, and P. virgatus). HPLC with monolithic reverse phase silica column (4.6 × 100 mm) and simple isocratic elution of methanol-water mixed with dioxane facilitated the separation of lignans of diverse nature such as diarylbutyrolactone, tetrahydrofuran, isomeric aryltetralin, and diarylbutane type for quantitative analysis. Targeted lignans viz. heliobuphthalmin lactone (1), virgatusin (2), hypophyllanthin (3), phyllanthin (4), nirtetralin (5), and niranthin (6) were confirmed unambiguously in four Phyllanthus species by their abundant molecular adduct ions, retention time, UV, and mass spectra as compared with those of reference compounds. Advantages and limitations of both detection techniques for qualitative (fingerprinting) and quantitative analysis of the above mentioned lignans in four Phyllanthus spp. are discussed. The method was validated following international guidelines. The described method can be utilized for assays and stability tests of P. amarus extracts as well as traditional Indian medicine based on Phyllanthus herb.

Słowa kluczowe

EN

Phyllanthus; lignans; LC-PDA-MS; method validation; fingerprinting

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2011
• Tom: Vol. 23, no. 2
• Strony: 321--337
• Opis fizyczny: Bibliogr. 45 poz., rys., tab.

Twórcy

autor

Shanker K.

• Council of Scientific and Industrial Research (CSIR) Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants Lucknow 226015 India

autor

Singh M.

• Council of Scientific and Industrial Research (CSIR) Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants Lucknow 226015 India

autor

Srivastava V.

• Council of Scientific and Industrial Research (CSIR) Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants Lucknow 226015 India

autor

Verma R. K.

• Council of Scientific and Industrial Research (CSIR) Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants Lucknow 226015 India

autor

Gupta A. K.

• Council of Scientific and Industrial Research (CSIR) Genetic Resource and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants Lucknow 226015 India

autor

Gupta M. M.

• Council of Scientific and Industrial Research (CSIR) Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants Lucknow 226015 India

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