Simultaneous determination of paeoniflorin and albiflorin in radix paeoniae rubra by HPLC—DAD—ELSD

• Autorzy: Zhu M. X. , Li S. N. , You H. D. , Han B. , Hu Y. X. , Li J. , Liu Y. F.

Identyfikatory

DOI

10.1556/1326.2017.29.2.12

• Języki publikacji: EN

Abstrakty

EN

High-performance liquid chromatography coupled with photodiode array detection and evaporative light scattering detection (HPLC—DAD—ELSD) was established to determine paeoniflorin and albiflorin simultaneously in Radix Paeoniae Rubra. The assay was performed on a Diamonsil C18 (4.6 mm × 250 mm, 5 μm) column by a gradient elution program with acetonitrile and aqueous formic acid (0.05% v/v) as mobile phase at a flow rate of 1.0 mL min−1. The detection wavelength of DAD was 230 nm, and the evaporator tube temperature of ELSD was set at 110 °C with the nebulizing gas flow rate of 3 L min−1. The temperature of column was kept at 30 °C. The linear ranges of paeoniflorin and albiflorin were within 0.050–1.510 mg mL−1 and 1.007–5.035 mg mL−1. The recoveries of paeoniflorin and albiflorin were 96.2–102.9% and 95.0–102.4%, respectively, while the relative standard deviation (RSD) of them was 0.2–2.5%. This method was quick, simple, accurate, and specific. It could be used for the quality control of Radix Paeoniae Rubra. The proposed approach was expected as a powerful tool for the quality control of Radix Paeoniae Rubra.

Słowa kluczowe

EN

HPLC-DAD-ELSD; Radix Paeoniae Rubra; paeoniflorin; albiflorin

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2017
• Tom: Vol. 29, no. 2
• Strony: 279--289
• Opis fizyczny: Bibliogr. 20 poz., rys.

Twórcy

autor

Zhu M. X.

• College of Pharmacy, Liaoning University, Shenyang 110036, P.R. China

autor

Li S. N.

• College of Pharmacy, Liaoning University, Shenyang 110036, P.R. China

autor

You H. D.

• Research Institute of Liaoning Province, Shenyang 110015, P.R. China

autor

Han B.

• College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, P.R. China
• College of Pharmacy, Liaoning University, Shenyang 110036, P.R. China

autor

Hu Y. X.

• College of Pharmacy, Liaoning University, Shenyang 110036, P.R. China

autor

Li J.

• College of Pharmacy, Liaoning University, Shenyang 110036, P.R. China

autor

Liu Y. F.

• College of Pharmacy, Liaoning University, Shenyang 110036, P.R. China
• Natural Products Pharmaceutical Engineering Technology Research Center of Liaoning Province, Shenyang 110036, P.R. China

Bibliografia

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Uwagi

PL

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę (zadania 2017).