Monitoring of pharmaceutical residues of non-steroidal drugs with use of Escherichia coli-gfp biosensors

• Autorzy: Matejczyk M. , Rosochacki S. J. , Jabłońska-Trypuć A.

Identyfikatory

DOI

10.2428/ecea.2016.23(1)4

Warianty tytułu

PL

Monitorowanie pozostałości farmaceutycznych leków niesterydowych z zastosowaniem Escherichia coli-gfp biosensorów

• Języki publikacji: EN

Abstrakty

EN

Escherichia coli strains containing a three different plasmid-borne transcriptional fusion between genotoxin-inducible recA, kat G and sodA promoters involved in the SOS regulon and bacteria stress response and mutated form of gfp reporter gene, have been used. GFP-based bacterial biosensors allowed for detection of a cytotoxic and genotoxic activity of ibuprofenum, ketoprofenum and paracetamolum – conventional non-steroidal anti-inflammatory drugs in PBS buffer and surface water. For experimental tests drugs were used at concentration of 10–6; 10–7; 10–8; 10–9 and 10–10 mg/dm3, with bacteria strains time incubation of 3 and 24 hours. Experimental data indicated, that three promoters fusions with gfp gene as reporter were differently sensitive to applied drugs. Bacteria strains, recA, kat G and sodA promoters were a good bioindicator for cytotoxic and genotoxic effect monitoring of tested drugs in PBS buffer and surface water. The results showed, that applied in this experiment E. coli gfp biosensors strains could be potentially useful for environmental monitoring of cytotoxic and genotoxic effect of pharmacist residues of drugs in surface water.

PL

W pracy wykorzystano szczepy Escherichia coli zawierające plazmidowe, trzy różne konstrukty genowe indukowalnych genotoksynami promotorów recA, kat G i sodA pochodzących z regulonu SOS oraz szlaków bakteryjnej odpowiedzi stresowej w fuzji z genem reporterowym gfp. GFP-bakteryjny biosensor pozwolił na detekcję cyto- i genotoksycznej aktywności ibuprofenu, ketoprofenu i paracetamolu – konwencjonalnych niesterydowych leków przeciwzapalnych w buforze PBS oraz wodzie powierzchniowej. Leki stosowano w stężeniach 10–6; 10–7; 10–8; 10–9 and 10–10 mg/dm3, z czasem inkubacji bakterii 3 i 24 godziny. Wyniki eksperymentu wykazały zróżnicowaną wrażliwość trzech różnych konstrukcji genowych na badane leki. Szczepy bakterii oraz RecA, kat G i sodA promotory okazały się dobrymi bioindykatorami monitorowanego cyto- i genotoksycznego efektu testowanych leków w buforze PBS i wodzie powierzchniowej. Uzyskane rezultaty wskazują na potencjalną użyteczność stosowanych w pracy bakteryjnych biosensorów w monitorowaniu pozostałości farmaceutycznych leków w środowisku.

Słowa kluczowe

EN

environmental monitoring; non-steroidal anti-inflammatory drugs; cytotoxicity; genotoxicity; gfp biosensor

PL

monitoring środowiskowy; niesterydowe przeciwzapalne leki; cytotoksyczność; genotoksyczność; GFP biosensor

• Wydawca: Towarzystwo Chemii i Inżynierii Ekologicznej
• Czasopismo: Ecological Chemistry and Engineering. A
• Rocznik: 2016
• Tom: Vol. 23, nr 1
• Strony: 45--62
• Opis fizyczny: Bibliogr. 55 poz., rys., tab.

Twórcy

autor

Matejczyk M.

• Department of Sanitary Biology and Biotechnology, Faculty of Civil Engineering and Environmental Engineering, Bialystok University of Technology, ul. Wiejska 45E, 15–351 Białystok, Poland; phone: [phone]; fax: [phone].

autor

Rosochacki S. J.

• Department of Sanitary Biology and Biotechnology, Faculty of Civil Engineering and Environmental Engineering, Bialystok University of Technology, ul. Wiejska 45E, 15–351 Białystok, Poland; phone: [phone]; fax: [phone].

autor

Jabłońska-Trypuć A.

• Department of Sanitary Biology and Biotechnology, Faculty of Civil Engineering and Environmental Engineering, Bialystok University of Technology, ul. Wiejska 45E, 15–351 Białystok, Poland; phone: [phone]; fax: [phone].

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Uwagi

PL

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę.