Future applications of apricot (Prunus armeniaca kaisa) ß galactosidase in dairy industry

• Autorzy: Ansarin S. A , Satar R. , Zaidi S. K. , Khan M. J. , Naseer M. I. , Al-Qahtani M. H. , Maskat M. Y.

Identyfikatory

DOI

10.2478/pjct-2014-0054

• Języki publikacji: EN

Abstrakty

EN

The present study demonstrates the immobilization of β galactosidase from apricots (Prunus armeniaca kaisa) on an inexpensive concanavalin A layered cellulose-alginate hybrid gel. Immobilized β galactosidase retained 78% of the initial activity after crosslinking by glutaraldehyde. It exhibited greater fraction of activity at both acidic and basic pH, and showed broad spectrum temperature optimum as compared to free enzyme. Moreover, immobilized enzyme exhibited higher thermal stability at 60^oC and retained 80% of the original enzyme activity in presence of 3% galactose. The crosslinked immobilized enzyme showed improved hydrolysis of lactose from milk and whey in batch processes at 50^oC as well as in continuous reactors operated at flow rate of 20 mL/h and 30 mL/h even after one month. Moreover, crosslinked adsorbed β galactosidase retained 76% activity even after its sixth repeated use, thereby promoting its use for lactose hydrolysis in various dairy products even for longer durations.

Słowa kluczowe

EN

apricot; β galactosidase; lactose hydrolysis; dairy industries

• Wydawca: West Pomeranian University of Technology. Publishing House
• Czasopismo: Polish Journal of Chemical Technology
• Rocznik: 2014
• Tom: Vol. 16, nr 3
• Strony: 74--79
• Opis fizyczny: Bibliogr. 28 poz., tab., wykr., wz.

Twórcy

autor

Ansarin S. A

• King Abdulaziz University, Center of Excellence in Genomic and Medicine Research, Jeddah-21589, Saudi Arabia

autor

Satar R.

• Ibn Sina National College for Medical Studies, Department of Biochemistry, Jeddah-21418, Saudi Arabia

autor

Zaidi S. K.

• King Abdulaziz University, Center of Excellence in Genomic and Medicine Research, Jeddah-21589, Saudi Arabia

autor

Khan M. J.

• Universiti Kebangsaan Malaysia, School of Chemical Sciences and Food Technology, Faculty of Science and Technology, 43600 Bangi, Selangor Darul Ehsan, Malaysia

autor

Naseer M. I.

• King Abdulaziz University, Center of Excellence in Genomic and Medicine Research, Jeddah-21589, Saudi Arabia

autor

Al-Qahtani M. H.

• King Abdulaziz University, Center of Excellence in Genomic and Medicine Research, Jeddah-21589, Saudi Arabia

autor

Maskat M. Y.

• Universiti Kebangsaan Malaysia, School of Chemical Sciences and Food Technology, Faculty of Science and Technology, 43600 Bangi, Selangor Darul Ehsan, Malaysia

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