Blood platelets apoptosis in hemodialyzed patients

• Autorzy: Sobol A. , Kamińska M. , Walczyńska M. , Stasiak M. , Szymański J. , Walkowiak M. , Walkowiak B.
• Języki publikacji: EN

Abstrakty

EN

Blood platelet proteome of hemodialyzed uremic patients exhibits significant difference in comparison to the blood platelet proteome of healthy subjects. This alteration is manifested by the presence of high concentrations of low molecular peptides within the whole range of pI. Increased platelet apoptosis has been put forward as a possible cause of this phenomenon (1). The aim of the present research was to assess whether blood platelet populations from hemodialyzed uremic patients exhibit more binding sites for Annexin V (a marker of apoptosis) than control samples from healthy donors. Blood was obtained from uremic patients immediately before and after hemodialysis. At the same time samples from control healthy donors were also collected. Blood was anticoagulated with sodium citrate and was immediately exposed to propidium iodide, fluorescent labeled Annexin V and CD61 antibodies. The samples were incubated for 10 minutes in the dark and next the labeled samples were processed in a BectonDickinson FACScan flow cytofluorymeter. Our preliminary study was performed for 12 hemodialyzed patients, 13nondialyzed uremic patientsand 12 controls. It was found that the blood platelet population of hemodialyzed patients exhibited significantly higher level of fluorescence intensity attributed to Annexin V. Furthermore, this intensity was comparable before and after hemodialysis and was independent on patient age. The results support the hypothesis that blood platelet contact with artificial surfaces during the process of hemodialysys may be partially responsible for triggering blood platelet apoptosis.

Słowa kluczowe

EN

blood platelets; hemodialysis; apoptosis

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2009
• Tom: Vol. 12, no. 89-91
• Strony: 29--30
• Opis fizyczny: Bibliogr. 1 poz.

Twórcy

autor

Sobol A.

• University Hospital no. 1, Medical University of Lodz,

autor

Kamińska M.

• Department of Biophysics, Technical University of Lodz

autor

Walczyńska M.

• Department of Biophysics, Technical University of Lodz

autor

Stasiak M.

• Department of Molecular and Medical Biophysics, Medical University of Lodz, Lodz, Poland

autor

Szymański J.

• Department of Molecular and Medical Biophysics, Medical University of Lodz, Lodz, Poland

autor

Walkowiak M.

• Department of Biophysics, Technical University of Lodz

autor

Walkowiak B.

• Department of Biophysics, Technical University of Lodz
• Department of Molecular and Medical Biophysics, Medical University of Lodz, Lodz, Poland

Bibliografia

• [1]. Walkowiak B, Kaminska M, Okroj W, Tanski W, Sobol A, Zbrog Z. Przybyszewska-Doros I, “Blood platelet proteome is changed in uremic patients” Platelets, 2007; 18(5): 386-388.