New liquid chromatography: Mass spectrometry assay for natural phytoestrogens from vegetable extracts

• Autorzy: Vlase L , Popa D.-S , Tero-Vescan A , Olah N
• Języki publikacji: EN

Abstrakty

EN

A new liquid chromatography tandem mass spectrometry (LC-MS/MS) assay for the quantification of seven isoflavones (daidzin, genistin, ononin, daidzein, glycitein, genistein, and formononetin) and coumestrol in vegetable extracts was developed. The separation was performed on a Zorbax SB-C18 column with a mixture of methanol (solvent A) and 0.1% (υ/υ) acetic acid in water (solvent B) under gradient conditions at 50°C with a flow rate of 1 mL min^-1. The detection of analytes was performed by electrospray ionization, negative ionisation, in non-reactive MS2 mode for aglycons or in reactive MS2 mode for glycosides. The method shows a good linearity (r2 > 0.9948) over the concentration range of 40–4000 ng mL^-1 for all analytes, a good precision (CV < 11%) and accuracy (<10%). The method was successfully applied to quantify the isoflavones and coumestrol in vegetable extracts obtained from red clover (Trifolium pratense L., Fabaceae) and dyer’s greenweed (Genista tinctoria L., Fabaceae) and can be used in the chemical characterization of vegetables with phytoestrogen content.

Słowa kluczowe

EN

phytoestrogens; isoflavones; LC-MS/MS; Trifolium pratense; Genista tinctoria

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2011
• Tom: Vol. 23, no. 3
• Strony: 509--520
• Opis fizyczny: Bibliogr. 23 poz., rys., tab.

Twórcy

autor

Vlase L

• “Iuliu Hatieganu” University of Medicine and Pharmacy Department of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy Emil Isac 13 RO-400023 Cluj-Napoca Romania

autor

Popa D.-S

• “Iuliu Hatieganu” University of Medicine and Pharmacy Department of Toxicology, Faculty of Pharmacy Emil Isac 13 RO-400023 Cluj-Napoca Romania

autor

Tero-Vescan A

• University of Medicine and Pharmacy Department of Pharmaceutical Biochemistry Gheorghe Marinescu 38 RO-540000 Targu-Mures Romania

autor

Olah N

• Vest University “Vasile Goldis” Arad and SC PlantExtrakt SRL Department of Drug Industry and Pharmaceutical Biotechnology, Faculty of Pharmacy RO-407059 Radaia, Cluj Romania

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