Phase features of several typical blood cells and their identification without unwrapping

• Autorzy: Wang Y. , Chen Y , Lü C. , Shang X. , Xu Y. , Wu H. , Zhu X. , Jin W.
• Języki publikacji: EN

Abstrakty

EN

The digital holographic phase microscopy (DHPM) technique which has been proposed for cellular morphology and dynamic analysis yielded highly desirable results. However, for nucleated cells (especially white blood cells (WBCs)), their submicroscopic structure has not yet been deconstructed through a phase unwrapping method due to the heterogeneity of an internal phase. By analyzing the phase heterogeneity of subclasses of WBCs, the typical phase models of them are built first in this paper; using the simulation method, the wrapped phase distributions of these models are obtained. However, by optimizing the wrapped phase maps and analyzing the relationships between them and typical blood cells, their features are selected and extracted. Then the models built are sorted out from each other successfully without unwrapping via analyzing these extracted features, which provides a valuable approach and technological base for the classification and identification of blood cells.

Słowa kluczowe

EN

digital holographic phase; nucleated cell; phase model; wrapped phase features; identification

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Optica Applicata
• Rocznik: 2013
• Tom: Vol. 43, nr 3
• Strony: 505--514
• Opis fizyczny: Bibliogr. 18 poz., rys., tab., wykr.

Twórcy

autor

Wang Y.

• Faculty of Science, Jiangsu University, Zhenjiang, Jiangsu 212013, China

autor

Chen Y

• Faculty of Science, Jiangsu University, Zhenjiang, Jiangsu 212013, China

autor

Lü C.

• Faculty of Science, Jiangsu University, Zhenjiang, Jiangsu 212013, China

autor

Shang X.

• Faculty of Science, Jiangsu University, Zhenjiang, Jiangsu 212013, China

autor

Xu Y.

• Faculty of Science, Jiangsu University, Zhenjiang, Jiangsu 212013, China

autor

Wu H.

• Faculty of Science, Jiangsu University, Zhenjiang, Jiangsu 212013, China

autor

Zhu X.

• School of Mechanical Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China

autor

Jin W.

• School of Mechanical Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China

Bibliografia

• [1] WANG Y.W., HAN G.C., LIU Y., CHENG X.N., WYROWSKI F., Simulation study on phase distribution of blood cells by virtual imitation, Chinese Journal of Lasers 36, 2009, pp. 1595–1600.
• [2] KEMPER B., KOSMEIER S., LANGEHANENBERG P., VON BALLY G., BREDEBUSCH I., DOMSCHKE W., SCHNEKENBURGER J., Integral refractive index determination of living suspension cells by multifocus digital holographic phase contrast microscopy, Journal of Biomedical Optics 12(5), 2007, article 054009.
• [3] RAPPAZ B., CANO E., COLOMB T., KÜHN J., DEPEURSINGE C., SIMANIS V., MAGISTRETTI P.J., MARQUET P., Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy, Journal of Biomedical Optics 14(3), 2009, article 034049.
• [4] KHMALADZE A., MATZ R.L., EPSTEIN T., JASENSKY J., BANASZAK HOLL M.M., ZHAN CHEN, Cell volume changes during apoptosis monitored in real time using digital holographic microscopy, Journal of Structural Biology 178(3), 2012, pp. 270–278.
• [5] XIAOXU LU, JIANPEI CHEN, SHENGDE LIU, ZHIJIAN MA, ZHUN ZHANG, LIYUN ZHONG, 3D profile reconstruction of biological sample by in-line image-plane phase-shifting digital microscopic holography, Optics and Lasers in Engineering 50(10), 2012, pp. 1431–1435.
• [6] POPESCU G., DEFLORES L.P., VAUGHAN J.C., BADIZADEGAN K., IWAI H., DASARI R.R., FELD M.S., Fourier phase microscopy for investigation of biological structures and dynamics, Optics Letters 29(21), 2004, pp. 2503–2505.
• [7] POPESCU G., BADIZADEGAN K., DASARI R.R., FELD M.S., Observation of dynamic subdomains in red blood cells, Journal of Biomedical Optics 11(4), 2006, article 040503.
• [8] POPESCU G., IKEDA T., BEST C.A., BADIZADEGAN K., DASARI R.R., FELD M.S., Erythrocyte structure and dynamics quantified by Hilbert phase microscopy, Journal of Biomedical Optics 10(6), 2005, article 060503.
• [9] LUE N., BEWERSDORF J., LESSARD M.D., BADIZADEGAN K., DASARI R.R., FELD M.S., POPESCU G., Tissue refractometry using Hilbert phase microscopy, Optics Letters 32(24), 2007, pp. 3522–3524.
• [10] LUE N., CHOI W., POPESCU G., YAQOOB Z., BADIZADEGAN K., DASARI R.R., FELD M.S., Live cell refractometry using Hilbert phase microscopy and confocal reflectance microscopy, Journal of Physical Chemistry A 113(47), 2009, pp. 13327–13330.
• [11] POPESCU G., IKEDA T., DASARI R.R., FELD M.S., Diffraction phase microscopy for quantifying cell structure and dynamics, Optics Letters 31(6), 2006, pp. 775–777.
• [12] YONGKEUN PARK, BEST C.A., BADIZADEGAN K., DASARI R.R., FELD M.S., KURIABOVA T., HENLE M.L., LEVINE A.J., POPESCU G., Measurement of red blood cell mechanics during morphological changes, Proceedings of the National Academy of Sciences of the United States of America 107(15), 2010, pp. 6731–6736.
• [13] BHADURI B., PHAM H., MIR M., POPESCU G., Diffraction phase microscopy with white light, Optics Letters 37(6), 2012, pp. 1094–1096.
• [14] RU WANG, ZHUO WANG, MILLET L., GILLETTE M.U., LEVINE A.J., POPESCU G., Dispersion-relation phase spectroscopy of intracellular transport, Optics Express 19(21), 2011, pp. 20571–20579.
• [15] WANG Y.W., HAN G.C., LIU Y., CHENG X.N., WYROWSKI F., Light scattering virtual simulation of red blood cell under double curve symmetrical model, Chinese Journal of Lasers 34, 2007, pp. 1676–1681.
• [16] XU Y.Y., WANG Y.W., JIN W.F., JIANG S.W., YUE Q.W., BU M., SHANG X.F., LV C.H., Study on phase characteristics of white blood cells and their optical models, Chinese Journal of Lasers 39, 2012, article 0504001.
• [17] WANG Y.W., LEI H.N., BU M., HAN G.C., Distribution characteristics and identification of several typical blood cells under optical phase models, Chinese Journal of Lasers 36, 2009, pp. 2629–2635.
• [18] YAWEI WANG, WEIFENG JIN, NAIFEI REN, Dual-medium quantitative measurement simulation on cells, Applied Optics 50(35), 2011, pp. 6440–6445.