Development and validation of RP-HPLC method for simultaneous analysis of andrographolide, phyllanthin, and hypophyllanthin from herbal hepatoprotective formulation

• Autorzy: Bhope S. G. , Kuber V. V. , Nagore D. H. , Gaikwad P. S. , Patil M. J.
• Języki publikacji: EN

Abstrakty

EN

A simple, sensitive, and accurate liquid chromatographic method with photodiode array detector was developed for the determination of andrographolide, phyllanthin, and hypophyllanthin. The separation was carried out on a reverse-phase 250 mm × 4.6 mm, 5μ symmetry C8 column (Waters). The gradient was prepared from 0.1% orthophosphoric acid (solvent A) and (1:1) acetonitrile:methanol (solvent B) as mobile phase delivered at a flow rate of 1 mL min ^-1. A linear behavior was observed between observed peak area response, and concentration of analytes was investigated, with good correlation coefficient. The method established was successfully applied to quantify andrographolide, phyllanthin, and hypophyllanthin from the herbal hepatoprotective formulation.

Słowa kluczowe

EN

HPLC; validation; phyllanthin; hypophyllanthin; andrographolide

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2013
• Tom: Vol. 25, no. 1
• Strony: 159--169
• Opis fizyczny: Bibliogr. 21 poz., rys., tab.

Twórcy

autor

Bhope S. G.

• MIDC Ranjangaon Analytical R&D, Tulip Lab Pvt. Ltd. F-20/21 Pune 412220 India

autor

Kuber V. V.

• MIDC Ranjangaon Analytical R&D, Tulip Lab Pvt. Ltd. F-20/21 Pune 412220 India

autor

Nagore D. H.

• MIDC Ranjangaon Analytical R&D, Tulip Lab Pvt. Ltd. F-20/21 Pune 412220 India

autor

Gaikwad P. S.

• MIDC Ranjangaon Analytical R&D, Tulip Lab Pvt. Ltd. F-20/21 Pune 412220 India

autor

Patil M. J.

• Marathwada Mitra Mandal College of Pharmacy Department of Pharmacognosy Pune India

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