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Oxalic acid is one of the important acids that is used in many fields. It is of medical, industrial and agricultural importance and is used as an acid in foods, building and construction, pharmacy, and others. This acid is produced in multiple ways, and the biogenic method is the best method because it is safe and cheap. Hence, this study came about, where reliance was placed on wheat bran in preparing nutrient medium to produce oxalic acid from Candida albicans and Aspergillus niger yeast isolated from environmental and pathogenic sources. The study aimed to use wheat bran as an alternative medium for growing fungi and yeasts that produce oxalic acid, and to compare the productivity of this medium with standard media. One hundred fungal isolates were isolated from different environmental and clinical sources, and grown in Sabouraud dextrose agar medium (SDA) and potato dextrose agar to obtain pure isolates of A. niger and C. albicans. Thirty seven isolates of C. albicans and thirty two isolates of A. niger were isolated. Thirty one contaminated samples were discarded. The isolates were grown in standard (SDB, PDB) and alternative (wheat bran) media. The amount of acid was estimated by mulching against potassium permanganate. The results showed that wheat bran medium was the most efficient in producing oxalic acid with a rate of 26.2% for A. niger and 25.3% for C. albicans, compared to standard media. The best temperature for acid production was 32 °C with a production rate of 19.1% for A. niger and 22.7% for C. albicans. The best pH was 6.5 for A. niger with a production rate of 20.2% and 5.5 for C. albicans with a production rate of 23.3%. The study conclude from the above that the fungus A. niger is the best compared to C. albicans, as well as the medium of wheat bran is a promising and effective medium in the production of oxalic acid in an environmentally friendly way.
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Tom
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230--235
Opis fizyczny
Bibliogr. 27 poz., rys., tab.
Twórcy
autor
- Department of Biology, College of Education, Al-Qadisiyah University, Iraq
autor
- Department of Biology, College of Education, Al-Qadisiyah University, Iraq
Bibliografia
- 1. Ahmed, F. and D. Cruickshank. 1953. A refinement of the crystal structure analyses of oxalic acid dihydrate. Acta Crystallographica, 6(5), 385–392.
- 2. Algburi, J.B., AL-Amari, M.J. 2023. The LC50 of Diazinon and sub-lethal concentration effect of it on hematological properties in Cyprinus Carpio fish. In AIP Conference Proceedings, 2830(1). AIP Publishing.
- 3. AL-mehana, W.N.A., AL-shibly, M.K., AL-daemi, F.H.F. 2021. Optimal conditions for oxalic acid production by Aspergillus niger and Aspergillus flavus. Iranian Journal of Ichthyology, 8, 138–144.
- 4. Al-Rikabi, A.A.F. and AL-Shibli, M.K.A. 2022. Evaluation of the ability of some local soil fungi to produce oxalic acid under different conditions.
- 5. Al-Shibly, M., AL-Jobouri, M.M., AL Sabaagh S.J. 2024. Preparation of an alternative medium composed of wheat bran to produce oxalic acid from Candida albicans. in AIP Conference Proceedings. AIP Publishing.
- 6. Alsudani, A.A. and Al-Shibli, M.K. 2015. Citric acid production from some local isolates of the fungus Aspergillus niger by rice husks filtrate medium. International Journal of Recent Scientific Research, 6(8), 5625–5633.
- 7. Brown, W. 1923. Experiments on the growth of fungi on culture media. Annals of Botany, 37(145), 105–129.
- 8. Chen, Z., Mense, A., Brewer, L., Shi, Y.-C. 2023. Wheat bran layers: Composition, structure, fractionation, and potential uses in foods. Critical reviews in food science and nutrition, 1–24.
- 9. Crowther, T.W., Boddy, L., Maynard, D.S. 2018. The use of artificial media in fungal ecology. Fungal Ecology, 32, 87–91.
- 10. Denning, D.W. 2024. Global incidence and mortality of severe fungal disease. The Lancet Infectious Diseases.
- 11. Frey, D., Oldfield, R.J., Bridger, R.C. 1979. A colour atlas of pathogenic Fungi.
- 12. Gadd, G.M. 1999. Fungal production of citric and oxalic acid: Importance in metal speciation, physiology and biogeochemical processes. Advances in Microbial Physiology, 41, 47–92.
- 13. Gautam, A.K. and Avasthi, S. 2019. Methods in Fungal Biology: A manual of Laboratory Protocols. Scientific Publishers.
- 14. Ghibate R., Ben Baaziz M., Chrachmy M., Kerrou M., Taouil R., Senhaji, O. 2024. Production of new activated carbon from agricultural waste and its use as an eco-friendly solution for removing Cu²⁺ ions from industrial effluents. Ecological Engineering & Environmental Technology.
- 15. Hodgkinson, A. 1977. Oxalic acid in biology and medicine. London—New York.
- 16. Huang, Y. 2023. The production of oxalate by aspergillus niger under different lead concentrations. Agronomy, 13(4), 1182.
- 17. Irfan, M., Nadeem, M., Syed, Q. 2012. Media optimization for amylase production in solid state fermentation of wheat bran by fungal strains. Journal of Cell & Molecular Biology, 10(1).
- 18. Kumar, S., Panwar, P., Sehrawat, N., Upadhyay, S.K., Sharma, A.K., Singh M. und Yadav M. 2024. Oxalic acid: Recent developments for cost-effective microbial production. Physical Sciences Reviews, 9(2), 891–907.
- 19. Mohsin, R.I., AL-Amari, M.J., Jaber, H.H. 2024. The toxicity of organophosphates insecticide in cyprinus carpio and effect on antioxidant and liver function. Ecological Engineering & Environmental Technology (EEET). 1, 25(7).
- 20. Onipe, O.O., Jideani, A.I., Beswa, D. 2015. Composition and functionality of wheat bran and its application in some cereal food products. International Journal of Food Science & Technology, 50(12), 2509–2518.
- 21. Pauwels, R., Graefe, J., Bitterlich, M. 2023. An arbuscular mycorrhizal fungus alters soil water retention and hydraulic conductivity in a soil texture specific way. Mycorrhiza, 33(3), 165–179.
- 22. Schuler, E., 2021. Towards sustainable oxalic acid from CO2 and biomass. ChemSusChem, 14(18), 3636–3664.
- 23. Stevenson, L., Schuler, E., Demetriou, M., Shiju,R., Gruter G.-J.M. 2012. Wheat bran: its composition and benefits to health, a European perspective. International Journal of Food Sciences and Nutrition, 63(8), 1001–1013.
- 24. Sudbery, P.E. 2011. Growth of Candida albicans hyphae. Nature Reviews Microbiology, 9(10), 737–748.
- 25. Wanzenböck, E., Apprich, S., Wien, F.H.C., Tirpanalan, Ö., Hannover, L., Zitz U. 2017. Wheat bran biodegradation by edible Pleurotus fungi – A sustainable perspective for food and feed. LWT, 86, 123–131.
- 26. Watanabe, T. 2002. Pictorial atlas of soil and seed fungi: morphologies of cultured fungi and key to species. CRC press.
- 27. Yakop, F., Taha, H., Shivanand, P. 2019. Isolation of fungi from various habitats and their possible bioremediation. Current Science, 116(5), 733–740.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-28f360ed-8448-4378-b234-016ca4e17db9
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