Extraction process and method validation for bioactive compounds from Citrus reticulata cv. Chachiensis : application of response surface methodology and HPLC–DAD

Mei, Zhenying; Zhang, Rongfei; Zhao, Zhimin; Zheng, Guodong; Xu, Xinjun; Yang, Depo

doi:10.1556/1326.2020.00789

Artykuł - szczegóły

Tytuł artykułu

Extraction process and method validation for bioactive compounds from Citrus reticulata cv. Chachiensis : application of response surface methodology and HPLC–DAD

Autorzy

Mei Zhenying , Zhang Rongfei , Zhao Zhimin , Zheng Guodong , Xu Xinjun , Yang Depo

Wybrane pełne teksty z tego czasopisma

Identyfikatory

DOI

10.1556/1326.2020.00789

Warianty tytułu

Języki publikacji

Abstrakty

Citrus reticulata cv. Chachiensis, a traditional Chinese herb, has extensive medicinal and edible effects. 3′,4′,5,6,7,8-Hexamethoxyflavone (HM) and 5,6,7,8,4′-pentamethoxyflavone (PM) are main bioactive compounds in Chachiensis, which have been reported to possess various biological properties. In this study, supercritical CO2 extraction (SCE) and high-speed countercurrent chromatography (HSCCC) were utilized to prepare HM and PM from Chachiensis. The contents of target compounds were determined by a high-performance liquid chromatography method with diode-array detection (HPLC-DAD), which was validated using the following parameters: linearity, sensitivity, repeatability, stability, precision and accuracy. The SCE conditions were optimized using response surface methodology with central composite design. Obtained optimum conditions were temperature of 37.9 °C, pressure of 26.3 MPa, and modifier volume of 81.0 mL. Under above conditions, the recoveries of target compounds were 92.52 ± 0.83 and 96.36 ± 0.43%, respectively. The most appropriate solvent system for HSCCC was selected as n-hexane/ethyl acetate/methanol/water (1:0.8:1:1.2, v/v). The HSCCC fractions were detected by HPLC-DAD, liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance spectroscopy (1H NMR and 13C NMR). The results indicated that this method was successfully applied to obtain HM and PM with high purities and high recoveries from Chachiensis.

Słowa kluczowe

HPLC-DAD HSCCC method validation extraction

Wydawca

University of Silesia in Katowice
Akademiai Kiado

Czasopismo

Acta Chromatographica

Rocznik

2021

Tom

Vol. 33, no. 3

Strony

270--280

Opis fizyczny

Bibliogr. 34 poz., rys., tab.

Twórcy

autor

Mei Zhenying

School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, China

autor

Zhang Rongfei

School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, China

autor

Zhao Zhimin

School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, China
Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou, 510006, China

autor

Zheng Guodong

School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou, 511436, China

autor

Xu Xinjun

School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, China
Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou, 510006, China

autor

Yang Depo

lssydp@mail.sysu.edu.cn

School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, China
Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou, 510006, China

https://orcid.org/0000-0001-6823-4135

Bibliografia

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Typ dokumentu

Bibliografia

Identyfikator YADDA

bwmeta1.element.baztech-26cb5bbf-505c-4654-bb5a-b2e66f26b8da