The sensitive and simple measurement of underivatized cholesterol and its oxygen derivatives in biological materials by capillary gas chromatography coupled to a mass-selective detector

• Autorzy: Czauderna M. , Marounek M. , Duskova D. , Kowalczyk J.
• Języki publikacji: EN

Abstrakty

EN

A method for determining underivatized cholesterol and its oxygen derivatives (ChlOs) in animal tissue specimens and some food has been developed using capillary gas chromatography (GC) and mass spectrometry (MS) with 5α-cholestane as the internal standard. The analysis preparation procedure for cholesterol and ChlOs consisted of lipid extraction followed by gentle saponification using 2 M KOH in ethanol. The analyzed samples were saponified overnight at room temperature in the dark with shaking. Then, free cholesterol and ChlOs were extracted using diethyl ether. The extracts were dried under argon, and the residues were redissolved in chloroform before GC-MS analysis. Cholesterol was analyzed in biological materials using the short column temperature program. The simultaneous quantification of cholesterol and its oxides in biological materials was performed using the longer column temperature program. Cholesterol was eluted faster than ChlOs. The chromatographic method that used column temperature program A can be successfully used for the simple and rapid quantification of cholesterol in biological materials, while the method using column temperature program B is more suitable for the simultaneous quantification of cholesterol and its oxides in biological materials.

Słowa kluczowe

EN

cholesterol; oxygen derivatives of cholesterol; gas chromatography; mass spectrometry; biological material

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2013
• Tom: Vol. 25, no. 4
• Strony: 655--667
• Opis fizyczny: Bibliogr. 21 poz., rys., tab.

Twórcy

autor

Czauderna M.

• Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland

autor

Marounek M.

• Prague-Uhříněves The Institute of Animal Science Prague Czech Republic
• Academy of Sciences of the Czech Republic The Institute of Animal Physiology and Genetics Prague Czech Republic

autor

Duskova D.

• Prague-Uhříněves The Institute of Animal Science Prague Czech Republic

autor

Kowalczyk J.

• Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland

Bibliografia

• [1] G. Leonarduzzi, B. Sottero, G. Poli, J. Nutr. Biochem., 13, 700 (2002)
• [2] M. Pieszka, Pol. J. Food Nutr. Sci., 57, 509 (2007)
• [3] M. Czauderna, J. Kowalczyk, K.M. Niedźwiedzka, Chem. Anal. (Warsaw), 53, 203 (2009)
• [4] S.R. Baggio, N. Bragagnolo, Food Chem., 95, 611 (2006)
• [5] M. Rudzińska, J. Korczak, E. Wąsowicz, Pol. J. Food Nutr. Sci., 14/55(4), 381 (2005)
• [6] B. Larkenson, P.C. Dutta, I. Hansson, J. AOAC Int., 77(6), 675 (2000)
• [7] J. Wilczak, G. Kulasek, Życie Wet., 79(9), 1 (2004)
• [8] L. Iuliano, Chem. Phys. Lipids, 164, 457 (2011)
• [9] M. Pieszka, P. Paściak, A. Janik, T. Barowicz, D. Wojtysiak, W. Migdal, J. Anim. Feed Sci., 15, 37 (2006)
• [10] M. Pieszka, K. Połtowicz, T. Barowicz, M. Pietras, Pol. J. Food Nutr. Sci., 13/14, 303 (2004)
• [11] F. Guardiola, R. Codony, P.B. Addis, M. Refecas, J. Boatella, Food Chem. Toxic., 2, 193 (1996)
• [12] R.C. Murphy, K.M. Johnson, J. Biol. Chem., 283, 15521 (2008)
• [13] K. Eder, G. Grünthal, H. Kluge, F. Hirche, J. Spilke, C. Brandsch, Br. J. Nutr., 93, 633 (2005)
• [14] A. Conchilio, D. Ansorena, I. Astiasaran, J. Sci. Food Agric., 85, 141 (2004)
• [15] R. Ringseis, K. Eder, J. Nutr., 132, 3732 (2002)
• [16] A-M. Lampi, L. Juntunen, J. Toivo, V. Piironen, J. Chromatogr. B, 777, 83 (2002)
• [17] E. Boselli, V. Velazco, M.F. Caboni, G. Lercker, J. Chromatogr. A, 917, 239 (2001)
• [18] A. Rowe, F.A.F. Macedo, J.V. Visentainer, N.E. Souza, M. Matsushita, Meat Sci., 51, 283 (1999)
• [19] V. Piironen, J. Toivo, A.-M. Lampi, J. Food Comp. Anal., 15, 705 (2002)
• [20] J. Flolch, M. Lees, G.H.S. Stanley, J. Biol. Chem., 226, 197 (1957)
• [21] J.T. Lin, T.A. McKeon, HPLC of Acyl Lipids, HNB Publishing, New York, 2005