Development and validation of rapid LC-MS with electrospray ionization for the quantification of pramipexole in human plasma

• Autorzy: Lavudu P. , Rani A. P. , Sekharan C. B.
• Języki publikacji: EN

Abstrakty

EN

A rapid, accurate and precise LC-MS method is described for the quantitative determination of pramipexole in human plasma matrix using ropinirole as internal standard. Pramipexole and ropinirole were extracted from plasma by liquid-liquid extraction technique. The method was validated over the concentration range of 100-2514 pg/mL. The method was found to have acceptable accuracy, precision, linearity and selectivity. The mean extraction recovery from spiked plasma samples was in the range of 79.415-87.00 %. The intra-day accuracy of the assay ranged from 98.924 to 112.236 % and intra-day precision ranged from 3.489 to 6.756 %. Inter-day accuracy and precision results for quality control samples ranged between 100.340 and 107.443% of nominal and precision is observed to be 3.970-5.714 %. The pramipexole was found to be stable after several stability studies. The proposed method yielded a quick, simple and reliable protocol for estimating pramipexole concentrations in human plasma.

Słowa kluczowe

EN

Pramipexole; LC-MS; human plasma; bioanalytical metod

• Wydawca: Przedsiębiorstwo Wydawnictw Naukowych "DARWIN"
• Czasopismo: International Letters of Chemistry, Physics and Astronomy
• Rocznik: 2014
• Tom: Vol. 18
• Strony: 1--15
• Opis fizyczny: Bibliogr. 11 poz., rys., tab.

Twórcy

autor

Lavudu P.

• Department of Pharmaceutical Biotechnology, Vishnu Institute of Pharmaceutical Education and Research, Narsapur, Andhra Pradesh – 502313, India

autor

Rani A. P.

• University College of Pharmaceutical Sciences, Acharya Nagarjuna University, Guntur, Andhra Pradesh – 522510, India

autor

Sekharan C. B.

• Department of Biotechnology, Jagarlamudi Kuppuswamy Choudary College, Guntur, Andhra Pradesh – 522006, India

Bibliografia

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