Development and validation of an HPTLC-densitometric method for the estimation of trigonelline in the leaves of Abrus precatorius L. and its formulation

• Autorzy: Kumbhalkar B. B , Rajopadhye A. A , Upadhye A. S
• Języki publikacji: EN

Abstrakty

EN

A new, simple, and reproducible HPTLC-densitometric method has been established and validated for estimation of trigonelline in the leaves of Abrus precatorius L. and its herbal formulation Chatak® using ICH guidelines. Accelerated solvent extraction (ASE) as an alternative to conventional techniques was also explored for rapid extraction. The methanol extracts of leaves, its formulation, and standard solution of trigonelline were applied on silica gel F254 HPTLC plates and developed in a twin chamber using the mobile phase toluene-ethyl acetate-formic acid-methanol (2:3:1.8:3.8, υ/υ/υ/υ). The plates were scanned at 268 nm (λmax of trigonelline) using a Camag TLC scanner 3 with the CATS 4 software. A linear relationship was obtained between the response (peak area) and the amount of trigonelline in the range 40–200 ng per spot; the correlation coefficient was 0.9957. The ASE method gives higher extraction efficiency in less time compared to Soxhlet extraction. The HPTLC-densitometric method showed good linearity, recovery, and high precision. It is useful to analyze trigonelline in A. precatorius leaves and routine quality control of its marketed formulation.

Słowa kluczowe

EN

trigonelline; Abrus precatorius L; accelerated solvent extraction; HPTLC-densitometry; ICH guidelines

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2012
• Tom: Vol. 24, no. 2
• Strony: 263--273
• Opis fizyczny: Bibliogr. 10 poz., rys., tab.

Twórcy

autor

Kumbhalkar B. B

• Agharkar Research Institute Botany Group, Plant Science Division G.G. Agarkar Road Pune 411 004 India

autor

Rajopadhye A. A

• Agharkar Research Institute Botany Group, Plant Science Division G.G. Agarkar Road Pune 411 004 India

autor

Upadhye A. S

• Agharkar Research Institute Botany Group, Plant Science Division G.G. Agarkar Road Pune 411 004 India

Bibliografia

• [1] The Wealth of India Raw Materials, Vol. 1: A, Publication & Information Directorate Council of Scientific & Industrial Research, New Delhi, India, 1985, pp. 18–20
• [2] S. Sankaranarayanan, P. Bama, J. Ramachandran, P.T. Kalaichelvan, M. Deccaraman, M. Vijayalakshimi, R. Dhamotharan, B. Dananjeyan, and S. Sathya Bama, J. Med. Plants Res., 4, 1089–1101 (2010)
• [3] Y.A. Ahirrao and D.A. Patil, Indian J. Nat. Prod. Resour., 1, 85–88 (2010)
• [4] O.A. Georgewill and U.O. Georgewill, Eastern J. Med., 14, 23–25 (2009)
• [5] J. Parekh and S.V. Chanda, Turk. J. Biol., 31, 53–58 (2007)
• [6] C. Wambebe and S.L. Amosun, J. Ethnopharmacol., 11, 49–58 (1984)
• [7] A.A. Adedapo, O.A. Omoloye, and O.G. Ohore, Onderstepoort J. Vet. Res., 74, 31–36 (2007)
• [8] S. Ghosal and S.K. Dutta, Phytochemistry, 10, 195–198 (1971)
• [9] C.L. Gopu, S.S. Gilda, A.R. Paradkar, and K.R. Mahadik, Acta Chromatogr., 20(4), 709–719 (2008)
• [10] I.C.H. Guidelines on Analytical Method Validation, in: Proceedings of International Convention on Quality for the Pharmaceutical Industry, Toronto, Canada, 2002