3D printed poly L-lactic acid (PLLA) scaffolds for nasal cartilage engineering

• Autorzy: Jabłoński A. , Kopeć J. , Jatteau S. , Ziąbka M. , Rajzer I.
• Języki publikacji: EN

Abstrakty

EN

In this study the scaffolds for nasal cartilages replacement were designed using a software called Rhino 3D v5.0. The software parameters considered for the design of scaffolds were chosen and the scaffolds were fabricated using Fused Deposition Modeling (FDM), a rapid prototyping technology, using poly(L-lactic acid) (PLLA) filament. The topographical properties of the scaffolds were calculated through 3D model simulation. The morphology of obtained scaffold was observed by Scanning Electron Microscopy (SEM). The biological properties, i.e. bioactivity of the scaffolds, were assessed in Simulated Body Fluid. On the basis of natural cartilages images the external shape of the scaffold was designed using the 3D modeling software. The FDM is a useful method in fabrication of 3D bioactive implants for cartilage tissue engineering. Thanks to the use of 3D modeling software, it is possible to prepare and manufacture artificial cartilage in a controlled manner. Artificial scaffold made of PLLA polymeric matrix may mimic natural one by shape, topography, geometry, pore size, and their distribution. In addition, it is possible to guarantee appropriately selected biological properties such as biocompatibility and high bioactivity of scaffolds, which was proved using scanning electron microscopy (SEM) analysis. The surface observation of the 3D printed scaffolds showed in vitro formation of apatite after immersion in the SBF. What is more, it is possible to match the scaffold not only to the large cavity but also individually to each patient.

Słowa kluczowe

EN

3D printing; PLLA; nasal cartilages; scaffolds; tissue engineering

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2018
• Tom: Vol. 21, no. 144
• Strony: 15--19
• Opis fizyczny: Bibliogr. 19 poz., rys., tab.

Twórcy

autor

Jabłoński A.

• ATH University of Bielsko-Biala, Faculty of Mechanical Engineering and Computer Science, Department of Mechanical Engineering Fundamentals, ul. Willowa 2, 43-309 Bielsko-Biała, Poland

autor

Kopeć J.

• ATH University of Bielsko-Biala, Faculty of Mechanical Engineering and Computer Science, Department of Mechanical Engineering Fundamentals, ul. Willowa 2, 43-309 Bielsko-Biała, Poland

autor

Jatteau S.

• ATH University of Bielsko-Biala, Faculty of Mechanical Engineering and Computer Science, Department of Mechanical Engineering Fundamentals, ul. Willowa 2, 43-309 Bielsko-Biała, Poland
• University of Lorraine, Polytech Nancy, 2 rue Jean Lamour, 2 rue Jean Lamour, 54519 Vandoeuvre les Nancy Cedex, Nancy, France

autor

Ziąbka M.

• AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Ceramics and Refractories, Al. Mickiewicza 30, 30-059 Krakow, Poland

autor

Rajzer I.

• ATH University of Bielsko-Biala, Faculty of Mechanical Engineering and Computer Science, Department of Mechanical Engineering Fundamentals, ul. Willowa 2, 43-309 Bielsko-Biała, Poland

Bibliografia

• [1] C. Chung, J.A. Burdick: Engineering cartilage tissue. Advanced Drug Delivery Reviews 60 (2008) 243-262.
• [2] K.K. Gómez-Lizárraga, C. Flores-Morales, M.L. Del Prado- -Audelo, M.A. Álvarez-Pérez, M.C. Piña-Barba, C. Escobedo: Polycaprolactone- and polycaprolactone/ceramic-based 3D-bioplotted porous scaffolds for bone regeneration: A comparative study. Materials Science and Engineering C 79 (2017) 326-335.
• [3] K. Haberstroh, et. al.: Bone repair by cell-seeded 3D-bioplotted composite scaffolds made of collagen treated tricalciumphosphate or tricalciumphosphate-chitosan-collagen hydrogel or PLGA in ovine critical-sized calvarial defects. J. Biomed. Mater. Res. B Appl. Biomater. 93 (2010) 520-530.
• [4] B. Derby: Printing and prototyping of tissues and scaffolds. Science 338 (2012) 921-926.
• [5] S. Yang, K.-F. Leong, Z. Du, C.-K. Chua: The design of scaffolds for use in tissue engineering. Part II. Rapid prototyping techniques. Tissue Eng. 8 (2002) 1-11.
• [6] B.C. Gross, J.L. Erkal, S.Y. Lockwood, C. Chen, D.M. Spence: Evaluation of 3D Printing and Its Potential Impact on Biotechnology and the Chemical Sciences. Anal. Chem. 86 (7) (2014) 3240-3253.
• [7] D. Dimitrov, K. Schreve, N. de Beer: Advances in Three Dimensional Printing - State of the Art and Future Perspectives. Rapid Prototyping Journal 12 (2006)136-147.
• [8] F. Rengier, A. Mehndiratta, H. et al.: 3D printing based on imaging data: review of medical applications. Int. J. Comput. Assist. Radiol. Surg. 5 (2010) 335-341.
• [9] S. Giannitelli, D. Accoto, M. Trombetta, A. Rainer: Current trends in the design of scaffolds for computer-aided tissue engineering. Acta Biomater. 10 (2) (2014) 580-594.
• [10] D.W. Hutmacher, M. Sittinger, M.V. Risbud: Scaffold-based tissue engineering: rationale for computer-aided design and solid free-form fabrication systems. Trends Biotechnol. 22 (2004) 354-362.
• [12] E. Sachs, M. Cima, J. Cornie: Three-dimensional printing: rapid tooling and prototypes directly from a CAD model, CIRP Ann. Manuf. Technol. 39 (1990) 201-204.
• [13] R. Landers, R. Mülhaupt: Desktop manufacturing of complex objects, prototypes and biomedical scaffolds by means of computer-assisted design combined with computer- guided 3D plotting of polymers and reactive oligomers. Macromol. Mater. Eng.282 (2000) 17-21.
• [15] F.P.W. Melchels, J. Feijen, D.W. Grijpma: A Poly(D,Llactide) Resin for the Preparation of Tissue Engineering Scaffolds by Stereolithography. Biomaterials 30 (2009) 3801-3809.
• [16] T. Kokubo, H. Takadama: How useful is SBF in predicting in vivo bone bioactivity? Biomaterials (2006) 2907-2915.
• [17] M. Emmert, P. Witzela, D. Heinrich: Challenges in tissue engineering – towards cell control inside artificial scaffolds. Soft Matter 12 (2016) 4287-4294.
• [18] R. Zhang, P.X. Ma: Porous poly(L-lactic acid)/apatite composites created by biomimetic process. J Biomed Mater Res 45 (1995) 285-293.
• [19] K. Zhang, Y. Wang, M.A. Hillmyer, L.F. Francis: Processing and properties of porous poly(L-lactide)/bioactive glass composites. Biomaterials 25 (2004) 2489-2500.

Uwagi

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2018).