Application of the silicon photomultiplier for fluorescence photobleaching measurement

• Autorzy: Mik Ł. , Kucewicz W.

Identyfikatory

DOI

10.5604/01.3001.0010.7556

• Języki publikacji: EN

Abstrakty

EN

In the paper a measurement method of fluorescence intensity reduction (called photobleaching) caused by excitation light was presented. Intensity of fluorescence light was measured by silicon photomultiplier (SiPM) – sensor which allows single photons detection. It has more compact dimensions and lower bias voltage in comparison to photomultiplier tube, presently used in many laboratory devices. Standard photometric cuvettes with a capacity of 1.6 ml and optical path length of 10 mm were used for the measurements. Sodium fluoresceinate dissolved in 10 mM TRIS buffer at pH 8.5 was used as the fluorescent dye. The solution was tested at a concentration of 100 μg per ml with constant excitation light from LED source over the time of measurement.

Słowa kluczowe

EN

silicon photomultiplier; fluorescence photobelaching; sodium fluoresceinate

PL

fotopowielacz krzemowy; fotobelkowanie fluorescencyjne; fluoresceinian sodu

• Wydawca: Państwowa Wyższa Szkoła Zawodowa w Tarnowie
• Czasopismo: Science, Technology and Innovation
• Rocznik: 2017
• Tom: Vol. 1, no. 1
• Strony: 11--16
• Opis fizyczny: Bibliogr. 8 poz., rys.

Twórcy

autor

Mik Ł.

• State Higher Vocational School in Tarnow, Mickiewicza 8, 33-100 Tarnów, Poland

autor

Kucewicz W.

• State Higher Vocational School in Tarnow, Mickiewicza 8, 33-100 Tarnów, Poland

Bibliografia

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