Influence of extraction time on collagen yield and proximate composition from yellowfin tuna (Thunnus albacares) bones: Insights from industrial waste valorization

• Autorzy: Prajaputra Vicky , Maryam Siti , Isnaini Nadia , Apriani Sahra , Maqfirah Siti , Lestari Allysa Salsabila , Mulyana Wilda Susanti

Identyfikatory

DOI

10.12912/27197050/194795

• Języki publikacji: EN

Abstrakty

EN

The valorization of tuna bones from industrial waste as a source of halal collagen presents a promising alternative to land-based collagen sources, such as bovine and porcine collagen. This study aims to evaluate the effect of extraction duration on the quality of collagen produced, focusing on yield, proximate composition (moisture, ash, fat, and protein content), functional group analysis, and collagen morphology. Collagen was extracted using the acid-soluble collagen (ASC) method, which involved pretreatment with NaOH to remove non-collagenous proteins, isopropyl alcohol to remove fat, and hydrolysis using acetic acid (CH₃COOH). The extraction durations tested were 24, 48, 72, and 96 hours, with a solution-to-sample ratio of 1:10 using 0.75 M CH₃COOH. Results showed that extraction for 96 hours yielded the highest collagen at 4.70%. FTIR analysis confirmed the presence of functional groups Amide I, II, III, A, and B, while SEM analysis revealed collagen morphology as small, rounded particles with fine pores and clearly visible collagen fibers. The moisture content decreased with longer extraction times, from 12.8% at 24 hours to 4.2% at 96 hours, while protein content increased, reaching 85.2% at 96 hours. The fat content was reduced to 0.2%, and ash content minimized to 0.8%. The proximate composition met the Indonesian National Standard (SNI 8076:2014), indicating that tuna bone collagen is a viable and good-quality source of halal collagen derived from industrial waste.

Słowa kluczowe

EN

by-product valorization; environmental chemistry; industrial waste management; marine biotechnology; marine chemistry; waste utilization; yellowfin tuna bone

• Wydawca: Lubelski Oddział Polskiego Towarzystwo Inżynierii Ekologicznej ; Lublin University of Technology ; WNGB Wydawnictwo Naukowe
• Czasopismo: Ecological Engineering & Environmental Technology
• Rocznik: 2025
• Tom: Vol. 26, iss. 1
• Strony: 1--7
• Opis fizyczny: Bibliogr. 27 poz., rys., tab.

Twórcy

autor

Prajaputra Vicky

• Department of Marine Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
• Atsiri Research Centre (ARC) PUI-PT Nilam Aceh, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia

• https://orcid.org/0000-0001-8838-9206

autor

Maryam Siti

• Department of Family Welfare Education, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia

• https://orcid.org/0009-0005-9914-7612

autor

Isnaini Nadia

• Atsiri Research Centre (ARC) PUI-PT Nilam Aceh, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
• Department of Pharmacy, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia

• https://orcid.org/0000-0001-6128-4316

autor

Apriani Sahra

• Department of Marine Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia

• https://orcid.org/0009-0009-2634-7292

autor

Maqfirah Siti

• Department of Marine Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia

• https://orcid.org/0009-0009-8217-2896

autor

Lestari Allysa Salsabila

• Department of Pharmacy, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia

• https://orcid.org/0009-0007-6816-1600

autor

Mulyana Wilda Susanti

• Department of Pharmacy, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia

• https://orcid.org/0009-0009-6327-0378

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