Rapid method for rhaponticin and deoxyrhaponticin separation and determination by TLC in Rheum rhaponticum L. and Rheum undulatum L.

• Autorzy: Smolarz H. D. , Hałka A , Chabros O , Dzido T. H.
• Języki publikacji: EN

Abstrakty

EN

The study describes the solvent extraction procedure and improved version of TLC method for the determination of rhaponticin and deoxyrhaponticin in rhizomes and petioles of two species of Rheum L. genus. Extracts from underground parts and petioles of plant were separated on thin layers of silica using a mixture of dichloromethane-95% ethanol-methanol (8:1:1). The bands of rhaponticin and deoxyrhaponticin were satisfactorily separated from the remaining extract components permitting their densitometric quantitative and qualitative determination. The concentration of these compounds in Rheum undulatum was higher than in Rheum rhaponticum. The amount of rhaponticin and deoxyrhaponticin ranged between 0.3 and 31.5 mg/g.

Słowa kluczowe

EN

planar chromatography; extraction; rheum; rhaponticin; deoxyrhaponticin

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2013
• Tom: Vol. 25, no. 1
• Strony: 127--134
• Opis fizyczny: Bibliogr. 15 poz., rys., tab.

Twórcy

autor

Smolarz H. D.

• Medical University of Lublin Chair and Department of Pharmaceutical Botany Lublin Poland

autor

Hałka A

• Medical University of Lublin Department of Physical Chemistry, Chair of Chemistry Lublin Poland

autor

Chabros O

• Medical University of Lublin Chair and Department of Pharmaceutical Botany Lublin Poland

autor

Dzido T. H.

• Medical University of Lublin Department of Physical Chemistry, Chair of Chemistry Lublin Poland

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