Constructing software for analysis of neuron, glial and endothelial cell numbers and density in histological Nissl-stained rodent brain tissue

• Autorzy: Kołodziejczyk A. , Ładniak M. , Piórkowski A.
• Języki publikacji: EN

Abstrakty

EN

Cell number, density and volume of white and gray matter in brain structures are not constant values. Cellular alterations in brain areas might coincide with neurological and psychiatric pathologies as well as with changes in brain functionality during selection experiments, pharmacological treatment or aging. Several softwares were created to facilitate quantitative analysis of brain tissues, however results obtained from these softwares require multiple manual settings making the computing process complex and time-consuming. This study attempts to establish half automated software for fast, ergonomic and an accurate analysis of cellular density, cell number and cellular surface in morphologically different brain areas: cerebral cortex, pond and cerebellum. Images of brain sections of bank voles stained with standard cresyl-violet technique (Nissl staining), were analyzed in designed software. Results were compared with other commercially available tools regarding number of steps to be done by user and number of parameters possible to measure.

Słowa kluczowe

EN

cell segmentation; cell-counting; image processing; image analysis

PL

segmentacja komórki; zliczanie komórek; przetwarzanie obrazu; analiza obrazu

• Wydawca: University of Silesia, Institute of Informatics, Computer Systems Department
• Czasopismo: Journal of Medical Informatics & Technologies
• Rocznik: 2014
• Tom: Vol. 23
• Strony: 77--85
• Opis fizyczny: Bibliogr. 31 poz., rys., tab.

Twórcy

autor

Kołodziejczyk A.

• Institute of Environmental Sciences, Jagiellonian University.

autor

Ładniak M.

• Department of Geoinfomatics and Applied Computer Science, AGH University of Science and Technology, A. Mickiewicza 30 Av., 30–059 Cracow, Poland

autor

Piórkowski A.

• Department of Geoinfomatics and Applied Computer Science, AGH University of Science and Technology, A. Mickiewicza 30 Av., 30–059 Cracow, Poland

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