Fingerprint of ethyl acetate extraction combined with qualitative and quantitative analysis on Patrinia scabra Bunge: Distinguish P. scabra Bunge from its confusable species

• Autorzy: Liu W. , Wei M. L , Wu Ch. Y , Zhu H. Y , Feng F , Xie N
• Języki publikacji: EN

Abstrakty

EN

Patrinia scabra Bunge has long been used in clinic as a traditional Chinese medicine for treating leukemia and cancer and regulating host immune response. Despite their wide use in China, no report on system analysis on their chemical constituents is available so far. The current study was designed to profile the fingerprint of ethyl acetate extract of it, and in addition, to characterize the major fingerprint peaks and determine their quantity. Therefore, a detailed gradient high-performance liquid chromatography was described to separate more than 30 compounds with satisfactory resolution in P. scabra Bunge. Based on the chromatograms of 10 batches samples, a typical high-performance liquid chromatographic (HPLC) fingerprint was established with 23 chromatographic peaks being assigned as common fingerprint peaks. Furthermore, a quadrupole time of flight mass spectrometry (Q-TOF/MS) was coupled for the characterization of major compound. As (+)-nortrachelogenin was the most predominant compound in P. scabra Bunge, the quantification on it was also carried out with the method being validated. As a result, (+)-nortrachelogenin was found to be from 1.33 to 2.21 mg g−1 in this plant material. This rapid and effective analytical method could be employed for quality assessment of P. scabra Bunge, as well as pharmaceutical products containing this herbal material.

Słowa kluczowe

EN

Patrinia scabra Bunge; fingerprint construction; quantitative analysis; method validation; HPLC-Q-TOF/MS

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2015
• Tom: Vol. 27, no. 1
• Strony: 177--187
• Opis fizyczny: Bibliogr. 13 poz., rys., tab.

Twórcy

autor

Liu W.

• China Pharmaceutical University Department of Pharmaceutical Analysis 24 Tongjiaxiang Nanjing 210009 China

autor

Wei M. L

• China Pharmaceutical University Department of Pharmaceutical Analysis 24 Tongjiaxiang Nanjing 210009 China

autor

Wu Ch. Y

• China Pharmaceutical University Department of Pharmaceutical Analysis 24 Tongjiaxiang Nanjing 210009 China

autor

Zhu H. Y

• China Pharmaceutical University Department of Pharmaceutical Analysis 24 Tongjiaxiang Nanjing 210009 China

autor

Feng F

• China Pharmaceutical University Department of Natural Medicinal Chemistry 24 Tongjiaxiang Nanjing 210009 China

autor

Xie N

• Jiangxi Qingfeng Pharmaceutical Ltd. Ganzhou 341000 China

Bibliografia

• [1] R.H. Liu, W.D. Zhang, Z.B Gu, C. Zhang, J. Su, and X.K. Xu, Nat. Prod. Res., 20, 866–870 (2006)
• [2] H. Sun, C. Sun, Y. Pan, Chem. Biodiversity, 2, 1351–1357 (2005)
• [3] J.H. Chen and X.X. Wang, J. Chin. Med. Mater., 31, 1689–1691 (2008)
• [4] Y. Konishi, T. Kiyota, C. Draghici, J.M. Gao, F. Yeboah, S. Acoca, S. Jarussophon, and E. Purisima, Anal. Chem., 79, 1187–1197 (2007)
• [5] F.L. Liu, F. Feng, and W.Y. Liu, Pharm. Clin. Res., 18, 356–359 (2010)
• [6] A. Kouno, I. Yasuda, H. Mizoshiri, T. Tanaka, N. Marubayashi, and D.M. Yang, Phytochemistry, 37, 467–472 (1994)
• [7] Q. Wu, Q. Yuan, E.H. Liu, L.W. Qi, Z.M. Bi, and P. Li, Biomed. Chromatogr., 24, 808–819 (2010)
• [8] G.L. Li, Y.A. Liu, J.B. Chang, C.L. Zhang, and J.X. Xie, J. Chin. Mass. Spectrom. Soc., 18, 27–34 (1997)
• [9] Z.B. Gu, X.J. Chen, G.J. Yang, T.Z. Li, W.Y. Liu, and W.D. Zhang, J. Chin. Med. Mater., 25, 178–180 (2002)
• [10] B. Vermes, O. Seligmann, and H. Wagner, Phytochemistry, 30, 3087–3089 (1991)
• [11] G.J. Yang, Z.B. Gu, W.Y. Liu, Y. Qiu, T.Z. Li, and W.D. Zhang, J. Asian Nat. Prod. Res., 6, 277–280 (2004)
• [12] J.Q. Mao, T.J. Li, Y. Qiu, Y.C. Rui, and Z.B. Gu, J. Pharm. Pract., 25, 10–12 (2007)
• [13] Z.B. Gu, G.J. Yang, H.Y. Cong, Y.X. Xu, H.S. Chen, and W.D. Zhang, Chin. Tradit. Herb. Drugs, 33, 781–782 (2002)