Hydroxyapatite as a support in protease immobilization process

• Autorzy: Zdarta J. , Budzinska K. , Kolodziejczak-Radzimska A. , Klapiszewski Ł. , Siwinska-Stefanska K. , Bartczak P. , Piasecki A. , Maciejewski H. , Jesionowski T.

Identyfikatory

DOI

10.5277/ppmp150222

• Języki publikacji: EN

Abstrakty

EN

Hydroxyapatite is used as a matrix for immobilization of protease from Aspergillus oryzae by a process of adsorption. The matrix obtained has the surface area of 26 m2/g and particles in the shape of flakes of diameters no greater than 650 nm. The efficiency of the proposed method was confirmed by the Fourier transform infrared spectroscopy, elemental analysis and by analysis of parameters of the pore structure of matrix and products after immobilization. On the basis of the Bradford method it was found that the greatest amount of enzyme (132 mg/g) was immobilized from a solution of initial enzyme concentration of 7 mg/cm3 after 24 h of the process.

Słowa kluczowe

EN

hydroxyapatite; enzyme immobilization; protease; physicochemical characteristic

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Physicochemical Problems of Mineral Processing
• Rocznik: 2015
• Tom: Vol. 51, iss. 2
• Strony: 633--646
• Opis fizyczny: Bibliogr. 40 poz., rys., tab.

Twórcy

autor

Zdarta J.

• Poznan University of Technology, Faculty of Chemical Technology, Institute of Chemical Technology and Engineering, Berdychowo 4, PL-60965, Poznan, Poland

autor

Budzinska K.

• Poznan University of Technology, Faculty of Chemical Technology, Institute of Chemical Technology and Engineering, Berdychowo 4, PL-60965, Poznan, Poland

autor

Kolodziejczak-Radzimska A.

• Poznan University of Technology, Faculty of Chemical Technology, Institute of Chemical Technology and Engineering, Berdychowo 4, PL-60965, Poznan, Poland

autor

Klapiszewski Ł.

• Poznan University of Technology, Faculty of Chemical Technology, Institute of Chemical Technology and Engineering, Berdychowo 4, PL-60965, Poznan, Poland

autor

Siwinska-Stefanska K.

• Poznan University of Technology, Faculty of Chemical Technology, Institute of Chemical Technology and Engineering, Berdychowo 4, PL-60965, Poznan, Poland

autor

Bartczak P.

• Poznan University of Technology, Faculty of Chemical Technology, Institute of Chemical Technology and Engineering, Berdychowo 4, PL-60965, Poznan, Poland,

autor

Piasecki A.

• Poznan University of Technology, Faculty of Mechanical Engineering and Management, Institute of Materials Science and Engineering, Jana Pawla II 24, PL-60965, Poznan, Poland

autor

Maciejewski H.

• Adam Mickiewicz University in Poznan, Faculty of Chemistry, Umultowska 89b, PL-61614 Poznan, Poland
• Poznan Science and Technology Park, A. Mickiewicz University Foundation, Rubiez 46, Poznan, Poland

autor

Jesionowski T.

• Poznan University of Technology, Faculty of Chemical Technology, Institute of Chemical Technology and Engineering, Berdychowo 4, PL-60965, Poznan, Poland

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