Bioeffects of silver nanoparticles (AgNPs) synthesized by producer of biosurfactant Bacillus subtilis strain : in vitro cytotoxicity, antioxidant properties and metabolic activities of mammalian cells

• Autorzy: Chojniak-Gronek Joanna Małgorzata , Jałowiecki Łukasz , Płaza Grażyna Anna

Identyfikatory

DOI

10.24425/aep.2022.143708

• Języki publikacji: EN

Abstrakty

EN

The present study is focused on the evaluation of bioeffects of silver nanoparticles (AgNPs) synthesized by Bacillus subtilis strain I’-1a, the producer of iturin A lipopeptide biosurfactant. The following properties of biologically synthesized silver nanoparticles (bio-AgNPs) were evaluated: in vitro cytotoxicity, antioxidant properties, and metabolic activities of mammalian cells. As a control, chemically synthesized silver nanoparticles (chem-AgNPs) were used. In vitro, antioxidant activity of bio-AgNPs showed a significant effect on the scavenging of free radicals. Bio-AgNPs can be potent natural antioxidants and can be essential for health preservation against oxidative stress-related degenerative diseases, such as cancer. The cell viability of human skin fibroblasts NHDF was remarkably inhibited in the presence of both AgNPs. However, bio-AgNPs were more active than chem-AgNPs. In our experiment, microarrays PM-M1–PM-M4 were used to evaluate the growth of NHDF fibroblast cells in the presence of bio-AgNPs and chem-AgNPs. The NHDF fibroblast cells were more active in the presence of bio-AgNPs than in chem-AgNPs. Probably, the presence of biosurfactant produced by Bacillus subtilis I’-1a significantly increased the stability of biogenic AgNPs and enhanced their biological activities and specific interaction with human DNA. Furthermore, the evaluated biological activities were enhanced for the biosurfactant-based AgNPs.

Słowa kluczowe

EN

green synthesis; bio-AgNPs; in vitro cytotoxicity; antioxidant properties; PM-Ms; Biolog system

• Wydawca: Institute of Environmental Engineering, Polish Academy of Sciences
• Czasopismo: Archives of Environmental Protection
• Rocznik: 2022
• Tom: Vol. 48, no. 4
• Strony: 45--52
• Opis fizyczny: Bibliogr. 32 s., rys., tab., wykr.

Twórcy

autor

Chojniak-Gronek Joanna Małgorzata

• Institute for Ecology of Industrial Areas, Poland
•  Łukasiewicz – Industrial Chemistry Institute, Poland

autor

Jałowiecki Łukasz

• Institute for Ecology of Industrial Areas, Poland

autor

Płaza Grażyna Anna

• Institute for Ecology of Industrial Areas, Poland

Bibliografia

• 1. Ahn, E-Y., Jin, H. & Park, Y. (2019). Green synthesis and biological activities of silver nanoparticles prepared by Carpesium cernuum extract. Arch. Pharm. Res. 7, 345. DOI:10.1007/s12272-019-01152-x.
• 2. Bernat, P., Paraszkiewicz, K., Siewiera, P., Moryl, M., Płaza, G. & Chojniak J. (2016). Lipid composition in a strain of Bacillus subtilis, a producer of iturin A lipopeptides that are active against uropathogenic bacteria. World J. Microbiol. Biotechnol. 32, 157. DOI:10.1007/s11274-016-2126-0.
• 3. Bochner, B.R., Siri, M., Huang, R.H., Noble, S., Lei, X.-H., Clemons, P.A. & Wagner, B.K. (2011). Assay of the multiple energy-producing pathways of mammalian cells. PLoS ONE 6:e18147. DOI:10.137/journal.pone.0018147.
• 4. Bodzek, M., Konieczny, K., Kwiecińska-Mydlak, A. (2021) New generation of semipermeable membranes with carbon nanotubes for water and wastewater treatment: Critical review. Archives of Environmental Protection: (47) 3 pp. 3-27. DOI:10.24425/aep.2021.138460
• 5. Chojniak, J., Libera, M., Król, E. & Płaza, G. (2018). A nonspecific synergistic effect of biogenic silver nanoparticles and biosurfactant towards environmental bacteria and fungi. Ecotoxicology 27, 352–359. DOI:10.1007/s10646-018-1899-3.
• 6. Durval, I.J.B., Meira, H.M., de Veras, B.O., Rufino, R.D., Converti, A. & Sarubbo, L.A.(2021). Green synthesis of silver nanoparticles using a biosurfactant from Bacillus cereus UCP 1615 as stabilizing agent and its application as an antifungal agent. Fermentation 7, 233. DOI:10.3390/fermentation7040233.
• 7. Giri, A.K., Jena, B., Biswal, B., Pradhan, A. K., Arakha, M., Acharya, S. & Acharya L. (2022). Green synthesis and characterization of silver nanoparticles using Eugenia roxburghii DC. extract and activity against bioflm producing bacteria. Scientifc Reports, 12, 8383. DOI:10.1038/s41598-022-12484-y.
• 8. George, D. & Mallery, P. (2010). SPSS for Windows Step by Step: A Simple Guide and Reference 17.0 Update.10th Edition, Pearson, Boston.
• 9. Grzegorzek, M. (2021) Nanofiltration usage for fluoride removal in the sodium chloride presence. Archives of Environmental Protection, (47) 4 pp. 98-108. DOI:10.24425/aep.2021.139506
• 10. Li, K., Du, S., Van Ginkel, S. & Chen Y. (2014). Atomic force microscopy study of the interaction of DNA and nanoparticles. Adv. Exp. Med. Biol. 811, 93-109. DOI:10.1007/978-94-017-8739-0_6.
• 11. Jadoun, S., Arif, R., Jangid, N.K. & Meena, R.K (2022). Green synthesis of nanoparticles using plant extracts: a review. Environm. Chemistry Letters 19, 355–374. DOI:10.1007/s10311-020-01074-x.
• 12. Jimoh, A.A. & Lin, J. (2019). Biosurfactant: A new frontier for greener technology and environmental sustainability. Ecotoxicol. Environ. Safety 184, 109607. DOI:10.1016/j.ecoenv.2019.109607.
• 13. Keat, C. L., Aziz, A., Eid, A. & Elmarzugi N. A. ( 2015). Biosynthesis of nanoparticles and silver nanoparticles. Biores. Bioprocess 2, 47-61. DOI:10.1186/s40643-015-0076-2.
• 14. Keshari, A.K., Srivastava, R., Singh, P., Yadav, V.B. & Nath G. (2020). Antioxidant and antibacterial activity of silver nanoparticles synthesized by Cestrum nocturnum. J. Ayurveda Integrat. Med. 11, 37e4. DOI:10.1016/j.jaim.2017.11.003.
• 15. Liao, C., Li, Y. & Tjong, S.C. (2019). Bactericidal and cytotoxic properties of silver nanoparticles. Int. J. Mol. Sci. 20, 449. DOI:10.3390/ijms20020449.
• 16. Mendrek, B., Chojniak, J., Libera, M., Trzebicka, B., Bernat, P., Paraszkiewicz, K. & Płaza G. (2016). Silver nanoparticles formed in bio- and chemical syntheses with biosurfactant as stabilizing agent. J. Disp. Sci. Technol. 38, 1647–1655. DOI:10.1080/01932691.2016.1272056.
• 17. Mensor, L.L., Menez, F.S., Leitão, G.G., Reis, A.S., Dos-Santos, T.C., Coube, C.S. & Leitao, S.G. (2001). Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytoteraphy Res. 15, 127-130. DOI:10.1002/ptr.687.
• 18. Pal, G., Rai, P. & Pandey A. (2019). Green synthesis of nanoparticles: A greener approach for a cleaner future. In: Green Synthesis, Characterization and Applications of Nanoparticles Micro and Nano Technologie. Shukla A.K. & Iravani S. (eds) Elsevier, pp. 1-26.
• 19. Pauly, R., Cascio, L., Srikanth, S., Jones, K., Sorrow, S., Cubillan, R., Chen, C.-F., Skinner, C.D., Champaigne, K., Stevenson, R.E., Schwartz, C.E. & Boccuto L.(2021). Development of a cell-based metabolic test for the identification of individuals with autism spectrum disorder. Res. Autism Spect. Disorders 85, 101790. DOI:10.1016/j.rasd.2021.101790.
• 20. Płaza, G.A., Pacwa-Płociniczak, M., Piotrowska-Seget, Z,. Brigmon, R. & Król, E. (2015). Characterization of Bacillus strains producing biosurfactants. [In:] Thangavel, P. & Sridevi, G. (eds). Environmental sustainability. Role of green technologies. Springer Science +Business Media: 173–183. DOI:10.1007/978-81-322- 2056-5_10.
• 21. Płaza, G.A., Chojniak, J., Mendrek, B., Trzebicka, B., Kvitek, L., Panacek, A., Prucek, R., Zboril, R., Paraszkiewicz, K. & Bernat, P. (2016). Synthesis of silver nanoparticles by Bacillus subtilis T-1 growing on agro-industrial wastes and producing biosurfactant. IET Nanobiotechnol. 10, 62–68. DOI:10.1049/iet-nbt.2015.0016.
• 22. riya, R.S., Geetha, D. & Ramesh, P.S. (2015). Antioxidant activity of chemically synthesized AgNPs and biosynthesized Pongamia pinnata leaf extract mediated AgNPs – A comparative study. Ecotoxicol. Environ. Safety 4, 234-252. DOI:10.1016/j.ecoenv.2015.07.037.
• 23. Rai, M., Ingle, A.P., Trzcińska-Wencel, J., Wypij, M., Bonde, S., Yadav, A., Kratošová, G. & Golińska, P. (2021). Biogenic silver nanoparticles: What we know and what do we need to know? Nanomaterials 11, 2901. DOI:10.3390/nano11112901.
• 24. Reddy, A.S., Chen, C.-Y., Baker, S.C., Chen, C.-C., Jean, J.-S., Fan, C.-W., Chen, H.-R., & Wang, J.-C. (2009). Synthesis of silver nanoparticles using surfactin: A biosurfactant as stabilizing agent. Mater. Lett. 63, 1227–1230. DOI:10.1016/j.matlet.2009.02.028.
• 25. Santhosh, P.B., Genova, J. & Chamati, H.(2022). Green synthesis of gold nanoparticles: An eco-friendly approach. Chemistry, 4, 345–369. DOI:10.3390/ chemistry4020026.
• 26. Selvakesavan, R.K. & Franklin, G. (2021). Prospective application of nanoparticles green synthesized using medicinal plant extracts as novel nanomedicines. Nanotechnol. Sc. Appl. 14, 179–195. DOI:10.2147/NSA.S5333467.
• 27. Shahzadi, I., Aziz Shah, S.M., Shah, M.M., Ismail, T., Fatima, N., Siddique, M., Waheed, U., Baig, A. & Ayaz, A. (2022). Antioxidant, cytotoxic, and antimicrobial potential of silver nanoparticles synthesized using Tradescantia pallida extract. Front. Bioeng. Biotechnol. 10, 907551. DOI:10.3389/fbioe.2022.907551.
• 28. Shreyash, N., Bajpai, S., Khan, M. A., Vijay, Y., Tiwary, S. K. & Sonker. M. (2021). Green synthesis of nanoparticles and their biomedical applications: A review. ACS Appl. Nano Mater. 4, 11428–11457. DOI:10.1021/acsanm.1c02946.
• 29. Tariq, H., Rafi, M., Amirzada, M. I., Muhammad, S. A., Yameen, M. A., Mannan, A., et al. (2022). Photodynamic cytotoxic and antibacterial evaluation of Tecoma stans and Narcissus tazetta mediated silver nanoparticles. Arabian J. Chem. 15, 103652. DOI:10.1016/j.arabjc.2021.103652.
• 30. Ying, S., Guan, Z., Ofoegbu, P.C., Clubb, P., Rico, C., He, F. & Hong, J. (2022). Green synthesis of nanoparticles: Current developments and limitations. Environm. Technol. Innovat. 26, 102336. DOI:10.1016/j.eti.2022.102336.
• 31. Yugal, K. Mohanta, Y.K.M., Panda, S.K., Jayabalan, R., Sharma, N., Bastia, A.K. & Mohanta, T.K. (2017). Antimicrobial, antioxidant and cytotoxic activity of silver nanoparticles synthesized by leaf extract of Erythrina suberosa (Roxb.). Front. Mol. Biosci. 4, 14. DOI:10.3389/fmolb.2017.00014.
• 32. Zhang, D., Ma, X.-L., Gu, Y., Huang, H. & Zhang.,G.-W. (2020). Green synthesis of metallic nanoparticles and their potential applications to treat Cancer. Front. Chem. 8, 799. DOI:10.3389/fchem.2020.00799.

Uwagi

PL

Opracowane ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023)