Influence of pH and Cellic® CTec2 enzymes dose on the glucose yield after enzymatic hydrolysis of cellulose at 45 °C

• Autorzy: Marchwicka Monika

Identyfikatory

DOI

10.5604/01.3001.0014.9045

• Języki publikacji: EN

Abstrakty

EN

Influence of pH and Cellic® CTec2 enzymes dose on the glucose yield after enzymatic hydrolysis of cellulose at 45 °C. The enzymatic hydrolysis with the use of industrial enzymes Cellic® CTec2 (Novozymes, Denmark) was carried out within the conditions recommended by the manufacturer and literature. Cellulose obtained by the Kürschner-Hoffer method from a wood of 3-year-old poplar (Populus trichocarpa) was used for the study. Three pH values of 4.8, 5.0 and 5.5 were applied. Also, three amounts of enzymes were used: 25, 50 and 100 mg per 100 mg of the dry mass of cellulose for each pH used. The temperature was 45 °C. Samples were taken after 24 h and subjected to chromatographic analysis to determine the glucose content in the hydrolysates, and then the process parameters allowing for the highest glucose yield after the enzymatic hydrolysis process. The highest glucose yield was obtained for pH 5.0 and 100 mg of enzymes per 100 mg of the dry mass of cellulose – 79 %.

PL

Wpływ pH i ilości enzymu Cellic® CTec2 na wydajnośc glukozy po hydrolizie enzymatycznej celulozy w 45 °C. Hydrolizę enzymatyczną z zastosowanie enzymów przemysłowych Cellic® CTec2 (Novozymes, Dania) przeprowadzono w warunkach zalecanych przez producenta i literaturę. Do badań wykorzystano celulozę otrzymaną metodą Kürschnera-Hoffera z drewna topoli trzyletniej Populus trichocarpa. Zastosowano trzy wartości pH: 4,8, 5,0 i 5,5. Zastosowano również trzy ilości enzymu: 25, 50 i 100 mg (na 100 mg suchej masy celulozy) dla każdego użytego pH. Temperatura wynosiła 45 °C. Próbki pobrano po 24 h i poddano analizie chromatograficznej w celu oznaczenia zawartości glukozy w hydrolizatach, a następnie parametrów procesu pozwalających na uzyskanie największej ilości glukozy po procesie hydrolizy enzymatycznej. Najwyższą wydajność glukozy uzyskano dla pH 5,0 i 100 mg enzymu na 100 mg suchej masy celulozy – 79 %.

Słowa kluczowe

EN

enzymes; cellulose

• Wydawca: Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
• Czasopismo: Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology
• Rocznik: 2020
• Tom: No. 112
• Strony: 85--91
• Opis fizyczny: Bibliogr. 19 poz., rys.

Twórcy

autor

Marchwicka Monika

• Warsaw University of Life Sciences-SGGW, Department of Wood Science and Wood Preservation, Institute of Wood Sciences and Furniture, Poland

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).