Simultaneous estimation of anti-cancer terpenoids in pharmaceutical nanoformulation by RP-HPLC and HPTLC

• Autorzy: Parveen R. , Ahmad F. J. , Iqbal Z. , Singh M. , Kamal Y. , Ahmad S.
• Języki publikacji: EN

Abstrakty

EN

Andrographolide and betulinic acid are the terpenoids having potential anti-cancer activity. The cytotoxicity activity of both the drugs was carried out separately and in combination on liver cancer HepG2 cell lines. High-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) methods were developed and validated for simultaneous estimation of these two terpenoids as per the International Conference on Harmonization (ICH) guidelines, which was applied for quantification in nanoformulation. The retention time by HPLC and retardation factor by HPTLC for andrographolide and betulinic acid were found to be 2.2 and 6.6 min, and 0.24 ± 0.01 and 0.66 ± 0.01, respectively. Both the methods were validated for accuracy, precision, repeatability, robustness, limit of detection (LOD), and limit of quantitation (LOQ). The content of andrographolide and betulinic acid in nanoformulation was found to be 96.0% and 98.0% by HPLC and 96.59% and 98.33% by HPTLC, respectively, of labelled claim.

Słowa kluczowe

EN

andrographolide; betulinic acid; high-performance liquid chromatography; high-performance thin-layer chromatography

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2014
• Tom: Vol. 26, no. 2
• Strony: 391--400
• Opis fizyczny: Bibliogr. 9 poz., rys., tab.

Twórcy

autor

Parveen R.

• Jamia Hamdard Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India

autor

Ahmad F. J.

• Jamia Hamdard Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India

autor

Iqbal Z.

• Jamia Hamdard Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India

autor

Singh M.

• Jamia Hamdard Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy New Delhi 110062 India

autor

Kamal Y.

• Jamia Hamdard Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy New Delhi 110062 India

autor

Ahmad S.

• Jamia Hamdard Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy New Delhi 110062 India

Bibliografia

• [1] A. Varma, H. Padh, and N. Shrivastava, eCAM, 1–9 (2011), Article ID 815390. doi: 10.1093/ecam/nep135
• [2] S. Fulda, Int. J. Mol. Sci., 9, 1096 (2008)
• [3] ICH, Q2 (R1), Validation of Analytical Procedures: Text and Methodology. International Conference on Harmonization, Geneva, 2005, http://www.ich.org/LOB/media/MEDIA417.pdf
• [4] J. Jaiswal, S. K. Gupta, and J. Kreuter, J. Control. Release, 96, 169 (2004)
• [5] R. Parveen, S. Baboota, J. Ali, A. Ahuja, S.S. Vasudev, and S. Ahmad, Int. J. Pharm., 413, 245 (2011)
• [6] S. Ahmad, R. Ahmad, R. Parveen, M. Mujeeb, and M. Aquil, Chromatographia, 67, 441 (2008)
• [7] M. Singh, Y.T. Kamal, R. Parveen, and S. Ahmad, J. Planar Chromatogr., 24, 172 (2011)
• [8] Y.T. Kamal, M. Singh, E.T. Tamboli, R. Parveen, and S. Ahmad, Acta Chromatogr., 23, 157 (2011)
• [9] R. Parveen, S. Baboota, J. Ali, A. Ahuja, and S. Ahmad, Biomed. Chromatogr. 24, 639 (2010)