Zastosowanie metod molekularnych w badaniach bioremediacji substancji ropopochodnych

• Autorzy: Brzeszcz J. , Kapusta P. , Turkiewicz A.

Warianty tytułu

EN

Application of molecular methods in bioremediation of petroleum substances

• Języki publikacji: PL

Abstrakty

PL

Zastosowanie nowoczesnych metod molekularnych zrewolucjonizowało dotychczasową wiedzę w zakresie filogenetycznej i funkcjonalnej różnorodności mikroorganizmów uczestniczących w procesach bioremediacji środowisk, w tym gleb, zanieczyszczonych substancjami ropopochodnymi. Ekosystem glebowy jest silnie zróżnicowanym układem ze stosunkowo wysokim poziomem prokariotycznej różnorodności. Tradycyjne techniki mikrobiologiczne, oparte na izolacji i hodowli w warunkach laboratoryjnych, nie zapewniają pełnego obrazu różnorodności drobnoustrojów występujących w środowiskach skażonych węglowodorami ropopochodnymi. Obecnie szacuje się, że klasyczne metody pozwalają izolować mniej niż 1% populacji mikroorganizmów glebowych. Tym samym zastosowanie metod molekularnych jako narzędzi diagnostycznych przyczyniło się do odkrycia nowych, dotąd niezidentyfikowanych mikroorganizmów. Dodatkowo techniki te oferują możliwość śledzenia zmian w zbiorowości mikroorganizmów w trakcie trwania procesów bioremediacji. W pracy przedstawiono przegląd nowoczesnych molekularnych technik mikrobiologicznych stosowanych w procesach bioremediacji środowisk zanieczyszczonych ksenobiotykami.

EN

Modern molecular techniques have greatly increased our knowledge concerning phylogenetic and functional diversity of microorganisms during bioremediation of oil-polluted environments such as soil. Soil ecosystem is relatively complex with a high level of prokaryotic diversity. The application of traditional culture-based techniques does not provide a full picture into phylogenetic and functional diversity of microbial community inhabiting oil-contaminated niches, since only a small fraction of the mentioned microbes may be cultivated in artificial media. Nowadays, less than 1% of these diverse microorganisms are cultivable by traditional cultivation techniques. Therefore, the use of molecular methods in studying microbial populations of hydrocarbon-polluted soils has led to the discovery of novel and unrecognized microorganisms. Such complex microbial diversity and dynamics in contaminated soil offer a resounding opportunity for bioremediation strategies. The paper presents an overview of modern approaches and applications of molecular microbiological techniques in bioremediation of petroleum-polluted environmental matrices. A general outline of the recent advances in this field is also given.

Słowa kluczowe

PL

metody molekularne; mikroorganizmy; bioremediacja; substancje ropopochodne

EN

molecular methods; microorganisms; bioremediation; petroleum substances

• Wydawca: Instytut Nafty i Gazu - Państwowy Instytut Badawczy
• Czasopismo: Nafta-Gaz
• Rocznik: 2013
• Tom: R. 69, nr 11
• Strony: 829--842
• Opis fizyczny: Bibliogr. 65 poz., il.

Twórcy

autor

Brzeszcz J.

• Zakład Mikrobiologii Instytut Nafty i Gazu ul. Lubicz 25A, 31-503 Kraków

autor

Kapusta P.

• Zakład Mikrobiologii Instytut Nafty i Gazu ul. Lubicz 25A, 31-503 Kraków

autor

Turkiewicz A.

• Zakład Mikrobiologii Instytut Nafty i Gazu ul. Lubicz 25A, 31-503 Kraków

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