Validated HPTLC method for simultaneous quantification of sennoside A, sennoside B, and kaempferol in Cassia fistula Linn.

• Autorzy: Bhope S. G , Kuber V. V , Nagore D. H
• Języki publikacji: EN

Abstrakty

EN

This paper describes a simple, precise, and accurate HPTLC method for simultaneous quantification of sennoside A, sennoside B, and kaempferol in Cassia fistula whole plant extract. Chromatographic separation of the sample extract was performed on aluminium foil plates coated with silica gel 60 F 254 as stationary phase. The mobile phase was toluene-ethyl acetate-methanol-formic acid 8:10:5:2 ( υ/υ ). Densitometric evaluation of the separated bands was performed at 270 nm. Sennosides A and B and kaempferol were satisfactorily resolved at R F 0.22 ± 0.05, 0.19 ± 0.05, and 0.81 ± 0.05, respectively. Recovery of sennosides A and B and kaempferol from Cassia fistula extract was 98.03, 98.74, and 99.08%, respectively. The method was validated for specificity, accuracy, linearity (100–400 ng per band), and precision (instrument precision in the range 1.03–1.33 and method precision in the range of 1.31–1.75) in accordance with ICH guidelines.

Słowa kluczowe

EN

HPTLC; sennoside A; sennoside B; kaempferol; Cassia fistula

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2010
• Tom: Vol. 22, no. 3
• Strony: 481--489
• Opis fizyczny: Bibliogr. 16 poz., rys., tab.

Twórcy

autor

Bhope S. G

• MIDC Ranjangaon Tulip Lab Pvt Ltd, F-20/21 Pune 412220 India

autor

Kuber V. V

• MIDC Ranjangaon Tulip Lab Pvt Ltd, F-20/21 Pune 412220 India

autor

Nagore D. H

• MIDC Ranjangaon Tulip Lab Pvt Ltd, F-20/21 Pune 412220 India

Bibliografia

• [1] Anonymous, The Wealth of India, Publication and Information Directorate New Delhi, 1992, pp. 337–343
• [2] K.R. Kirtikar and B.A. Basu (eds), Indian Medicinal Plants, Periodical Experts Book Agency, New Delhi, 1991, pp. 856–860
• [3] T. Bhakta, P.K. Mukherjee, M. Pal, and B.P. Saha, Pharm. Biol., 36 , 140 (1998)
• [4] T. Bhakta, P.K. Mukherjee, M. Pal, and B.P. Saha, Nat. Prod. Sci., 4 , 84 (1998)
• [5] M.S. Kumar, R. Sripriya, H.V. Raghavan, and P.K. Sehgal, J. Surg. Res., 131 , 283 (2006)
• [6] V. Duraipandiyan and S. Ignacimuthu, J. Ethnopharmacol., 112 , 590 (2007)
• [7] P. Siddhuraju, P.S. Mohan, and K. Becker, Food Chem., 79 , 61 (2002)
• [8] M. Govindarajan, A. Jebanesan, and T. Pushpanathan, Parasitol. Res., 102 , 289 (2008)
• [9] K. Pradeep, C.V. Mohan, K. Gobianand, and S. Karthikeyan, Chem. Biol. Interact., 167 , 12 (2007)
• [10] N.B. Chaudhari, K.P. Chittam, and V.R. Patil, Arch. Pharm. Sci. Res., 1 , 218 (2009)
• [11] V. Narayanan and T.R. Seshadri, Indian J. Chem., 10 , 379 (1972)
• [12] L. Ching-Kuo, Y. Chungb, F. Hsua, and Y. Kuob, Chin. Pharm. J., 55 , 231 (2003)
• [13] B. Theeshan, S.N. Vidushi, and I.A Okezie, Afr. J. Biotechol., 13 , 1530 (2005)
• [14] ICH, Q2A, Harmonised tripartite guideline, text on validation of analytical procedures, IFPMA, in: Proceedings of the International Conference on Harmonization, Geneva, 1994, pp. 1–5
• [15] V. Narayanan and T.R. Seshadri, Indian J. Chem., 10 , 379 (1972)
• [16] X. Theeshan, S. Vidushi, O. Neergheen, and I. Aruoma., Afr. J. Biotechnol., 13 , 1530 (2005)