Propagation of non-adherent HL-60 cells in batch cultures maintained in staticand wave-type agitated systems

• Autorzy: Wierzchowski Kamil , Grabowska Iwona , Pilarek Maciej

Identyfikatory

DOI

10.24425/cpe.2019.126109

• Języki publikacji: EN

Abstrakty

EN

Typically applied static (i.e. non-agitated) cultures do not provide sufficient conditions for efficient propagation of suspended non-adherent cells, in general. Feasibility of small-scale wave-type agitated single-use bioreactors for gentle agitation underlies applicability of such systems for scaling-up of fragile biomass of animal cells. The basic aim of the study was to compare the results of non-adherent HL-60 cell propagation performed referentially as the batch culture in typical static (i.e. non-agitated) disposable culture flasks (50 cm3 of culture medium) and in ReadyToProcess WAVETM25 bioreactor system (GE Healthcare) equipped with disposable culture bag (300 cm3 of culture medium) subjected to continuous wave-type agitation. The density and viability of HL-60 cells were significantly higher for the bioprocess subjected to wave-type agitation, than in the reference static culture. The values of the specific rate of glucose consumption per cell (rglc=cell) exhibited by HL-60 cells maintained in the system with continuous wave-type agitation was significantly lower (i.e. up to more than 42%) than the values noted for the static culture, for exactly the same time-points of two compared cultures. The results of the studies undoubtedly and comprehensively confirmed the applicability of the studied disposable bioreactor with wave-induced agitation as the right platform for proceeding the propagation of non- adherent HL-60 cells and for providing the culture conditions required by HL-60 cells for sustainable metabolism.

Słowa kluczowe

EN

disposable (single-use) bioreactor; wave-typeagitation; non-adherentcellculture; human HL-60cells

PL

bioreaktor jednorazowego użytku; falowanie; nieprzylegająca hodowla komórkowa; ludzkie komórki HL-60

• Wydawca: Komitet Inżynierii Chemicznej i Procesowej Polskiej Akademii Nauk
• Czasopismo: Chemical and Process Engineering
• Rocznik: 2019
• Tom: Vol. 40, nr 2
• Strony: 167–--177
• Opis fizyczny: Bibliogr. 15 poz., rys., tab.

Twórcy

autor

Wierzchowski Kamil

• Warsaw University of Technology, Faculty of Chemical and Process Engineering, Waryńskiego 1,00-645 Warsaw, Poland

autor

Grabowska Iwona

• University of Warsaw, Faculty of Biology, Miecznikowa 1, 02-096 Warsaw, Poland

autor

Pilarek Maciej

• Warsaw University of Technology, Faculty of Chemical and Process Engineering, Waryńskiego 1,00-645 Warsaw, Poland

Bibliografia

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Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).