Chromatographic fractionation of betacyanins from flowers of Gomphrena globosa

• Autorzy: Szot D. , Starzak K. , Skopińska A. , Wybraniec S.
• Języki publikacji: EN

Abstrakty

EN

In this study, a chromatographic fractionation of betacyanin pigments from extract of purple Gomphrena globosa petals was performed by preparative high performance liquid chromatography (prep-HPLC). The particular betacyanins in each collected fractions were tentatively identified by chromatography with optical amd mass spetrometric detection (LC-DAD-ESI-MS). Betacyanins are natural pigments, which are confirmed to have an antioxidant activity. It was reported that betacyanins can prevent civilization diseases, because of an ability of free radicals scavenging. Betacyanins are present in tissue of plants from Amaranthaceae family, e.g. in petals of Gomphrena globosa. Preparative separation of these particular pigments is difficult, because they are structurally very similar to each other and tend to coelute. However, in this study, fractions, containing a dominant amount of the principal pigments of Gomphrena globosa inflorescences, were obtained successfully. In the first fractions, two isomeric pigments assigned to gomphrenin I and isogomphrenin I were detected. The fractions of significant amounts of gomphrenin III and isogomphrenin III were obtained separately with sufficient purity. The presence of other, minor gomphrenin-type betacyanins were also confirmed as: gomphrenin II, cis-isomer of gomphrenin II and sinapoyl-gomphrenin I as well as their 15S-diastereomers. Moreover, in other fractions, various unknown pigments were detected.

Słowa kluczowe

EN

betacyanins; Gomphrena globosa; plant pigments; LC-MS

PL

betacyjaniny; Gomfrena kulista; barwniki roślinne; LC-MS

• Wydawca: Foundation for Young Scientists
• Czasopismo: Challenges of Modern Technology
• Rocznik: 2015
• Tom: Vol. 6, no. 2
• Strony: 19--23
• Opis fizyczny: Bibliogr. 7 poz., rys., tab., wykr.

Twórcy

autor

Szot D.

• Department of Analytical Chemistry, Institute C-1, Faculty of Chemical Engineering and Technology, Cracow University of Technology, ul. Warszawska 24, Cracow 31-155, Poland

autor

Starzak K.

• Department of Analytical Chemistry, Institute C-1, Faculty of Chemical Engineering and Technology, Cracow University of Technology, ul. Warszawska 24, Cracow 31-155, Poland

autor

Skopińska A.

• Department of Analytical Chemistry, Institute C-1, Faculty of Chemical Engineering and Technology, Cracow University of Technology, ul. Warszawska 24, Cracow 31-155, Poland

autor

Wybraniec S.

• Department of Analytical Chemistry, Institute C-1, Faculty of Chemical Engineering and Technology, Cracow University of Technology, ul. Warszawska 24, Cracow 31-155, Poland

Bibliografia

• [1] D.Strack., “Methods in Plant Biochemistry,” vol. 8, pp. 421-450, 1993
• [2] T.J. Mabry, “The conversion of betanidin and betanin to neobetanin derivatives,” Tetrahedron, 23, pp. 3111-3127, 1967
• [3] D.A. Moreno, “Betalains in the era of global agri-food science, technology and nutritional health,” Phytochem Rev. 7, pp. 261-262, 2008
• [4] G. J. Kapadia, “Chemoprevention of DMBA-induced UV-B promoted, NOR-1-induced TPA promoted skin carcinogenesis, and DEN-induced phenobarbital promoted liver tumors in mice by extract of beetroot,” Pharmacol Res. 47 (2), pp. 141-148, 2003
• [5] L. Minale, “Pigments of Centrospermae – VII. Betacyanins from Gomphrena Globosa L,” Phytochemistry, 6, pp. 703-709 1967
• [6] S. Heuter, “Betacyanins from flowers of Gomphrena Globosa,” Phytochemistry, 31(5), pp. 1801-1807, 1992
• [7] F. Kugler “Characterisation of betalain patterns of differently coloured inflorescences from Gomphrena globosa L. and Bougainvillea sp. by HPLC-DAD-ESI-MSn,” Anal. Bioanal. Chem. 387, pp. 637-648, 2007