Nowe termowrażliwe powierzchnie polimerowe do hodowli i uwalniania komórek skóry

• Autorzy: Oleszko-Torbus N. , Utrata-Wesołek A. , Wałach W. , Dworak A. , Trzebicka B.

Warianty tytułu

EN

New thermoresponsive polymer layers for skin cell culture and detachment

• Języki publikacji: PL

Abstrakty

EN

Covalent attachment of a thermoresponsive polymer to solid support leads to layers exhibiting temperature-dependent properties. Below the cloud point temperature (T_CP) of the thermoresponsive polymer the layer is hydrophilic – it is hydrated and polymer chains adopt an expanded conformation. Above T_CP, the polymer chains collapse due to dehydration and the surface becomes hydrophobic. This is a reversible process, lowering the temperature cause hydration and swelling of the layer. Such thermoresponsive layers can be obtained via reactions of entities present on the surface (e.g. functional groups, radicals etc.) with complementary functionalities in the polymer chains (grafting to) or with monomer subjected to polymerization (grafting from). Thermoresponsive layers may be used in many biomedical applications such as separation of molecules or cell sheet engineering. In this work, well-defined thermoresponsive (co)polymers of glycidol and ethyl glycidyl carbamate (mPGl), 2-ethyl and 2-nonyl-2-oxazoline (PENOx) as well as homopolymers of 2-isopropyl-2-oxazoline (PIPOx) were grafted to functionalized glass and silica substrates with the aim to obtain thermoresponsive layers for potential application in cell sheet engineering. Presence of polymers covalently bonded to substrates was confirmed by FT-IR and XPS studies. The polymer layers were 5-50 nm thick, depending on the molar mass and polymer concentration. Microscopic techniques indicated a smooth surface of mPGl layers, slightly rough texture of PENOx layers and fibrille-like fibers surface of PIPOx layers. Ellipsometry and contact angle studies revealed the response of layers to temperature changes. Biocompatibility of layers with dermal fibroblasts was confirmed by toxicity tests. Thermoresponsive surfaces were employed as substrates for skin cell culture and harvesting. Fibroblasts adhesion and proliferation on investigated surfaces was comparable with control sample. A confluent cell sheet was formed after 24 hours of culture. The influence of surface properties on cell adhesion and proliferation was examined. Detachment of cells from surfaces was controlled by variation of the temperature. An intact monolayer of cultured dermal fibroblasts was detached. No mechanical or enzymatic methods were required to harvest the cell sheets. Skin cell sheets, detached from thermoresponsive polymer layers may be applied in the cell sheet engineering that is highly desirable in tissue regeneration.

Słowa kluczowe

PL

powierzchnia polimerowa; termowrażliwe powierzchnie polimerowe; biomateriały; warstwa komórkowa; inżynieria warstw komórkowych; inteligentne polimery

EN

polymer surface; thermoresponsive polymer surfaces; biomaterials; cell sheet engineering; intelligent polymers

• Wydawca: Polskie Towarzystwo Chemiczne
• Czasopismo: Wiadomości Chemiczne
• Rocznik: 2016
• Tom: [Z] 70, 11-12
• Strony: 803--818
• Opis fizyczny: Bibliogr. 10 poz., fot., rys., schem.

Twórcy

autor

Oleszko-Torbus N.

• Centrum Materiałów Polimerowych i Węglowych Polskiej Akademii Nauk, ul. M. Curie-Skłodowskiej 34, 41-819 Zabrze

autor

Utrata-Wesołek A.

• Centrum Materiałów Polimerowych i Węglowych Polskiej Akademii Nauk, ul. M. Curie-Skłodowskiej 34, 41-819 Zabrze

autor

Wałach W.

• Centrum Materiałów Polimerowych i Węglowych Polskiej Akademii Nauk, ul. M. Curie-Skłodowskiej 34, 41-819 Zabrze

autor

Dworak A.

• Centrum Materiałów Polimerowych i Węglowych Polskiej Akademii Nauk, ul. M. Curie-Skłodowskiej 34, 41-819 Zabrze

autor

Trzebicka B.

• Centrum Materiałów Polimerowych i Węglowych Polskiej Akademii Nauk, ul. M. Curie-Skłodowskiej 34, 41-819 Zabrze

Bibliografia

• [1] M. Heskins, J.E. Guillet, J. Macromol. Sci., Part A: Pure Appl. Chem., 1968, 2, 1444.
• [2] T. Chen, R. Ferris, J. Zhang, R. Ducker, S. Zauscher, Prog. Polym. Sci., 2010, 35, 94.
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• [4] N. Yamada, T. Okano, H. Sakai, F. Karikusa, Y. Sawasaki, Y. Sakurai, Makromol. Chem. Rapid Commun., 1990, 11, 571.
• [5] L.Q. Chu, X.N. Zou, W. Knoll, R. Förch, Surf. Coat. Tech., 2008, 202, 2047.
• [6] M. Jamróz-Piegza, A. Utrata-Wesołek, B. Trzebicka, A. Dworak, Eur. Polym. J., 2006, 42, 2497.
• [7] K. Aoi, M. Okada, Prog. Polym. Sci., 1996, 21, 151.
• [8] M.W.M. Fijten, J.M. Kranenburg, H.M.L. Thijs, R.M. Paulus, B.M. van Lankvelt, J. de Hullu, M. Springintveld, D.J.G. Thielen, C.A. Tweedie, R. Hoogenboom, K.J. Van Vliet, U.S. Schubert, Macromolecules, 2007, 40, 5879.
• [9] T. Chen, R. Ferris, J. Zhang, R. Ducker, S. Zauscher, Prog. Polym. Sci., 2010, 35, 94.
• [10] J. O’Brien, I. Wilson, T. Orton, F. Pognan, Eur. J. Biochem., 2000, 267, 5421.

Uwagi

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę (zadania 2017).