Simultaneous determination of five flavonoids in Saussurea mongolica Franch by high-performance liquid chromatography

• Autorzy: Chen J. , Shi Y. P.
• Języki publikacji: EN

Abstrakty

EN

A high-performance liquid chromatographic (HPLC) technique coupled with photodiode array (PDA) detection has been proposed for simultaneous determination of five flavonoids, i.e. quercetin 3-O-β-D-glucopyranoside, quercetin 4′-methoxy-3-O-β-D-galactopyranoside, kaempferol 3-O-β-L-rhamnopyranoside, asebotin, and kaempferol 7-methxoy-3-O-α-L-rhamnopyranoside in extract of the whole plant of Saussurea mongolica Franch. The optimum conditions for separation were achieved on a 4.6 × 250 mm i.d., 5-μm particle, C18 column with acetonitrile and 1% acetic acid (20:80, v/v) as the mobile phase at a flow rate of 1.0 mL min−1. For all the analytes, a good linear regression relationship (r of >0.999) was obtained between peak area and concentration over a relatively wide range. The method was validated for repeatability, precision, stability, and accuracy. Seven different extraction procedures were investigated for preparation of the sample solution. The validated method was successfully applied to simultaneous analysis of these flavonoids in S. mongolica and was found to be simple and efficient.

Słowa kluczowe

EN

phytochemical analysis; high-performance liquid chromatography; Saussurea mongolica; flavonoids

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2012
• Tom: Vol. 24, no. 3
• Strony: 489--499
• Opis fizyczny: Bibliogr. 25 poz., rys., tab.

Twórcy

autor

Chen J.

• Chinese Academy of Sciences Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P. R. China

autor

Shi Y. P.

• Chinese Academy of Sciences Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P. R. China

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