3D printed Polylactid Acid based porous scaffold for bone tissue engineering: an in vitro study

• Autorzy: Bodnárová Simona , Gromošová Sylvia , Hudák Radovan , Rosocha Ján , Živčák Jozef , Plšíková Jana , Vojtko Marek , Tóth Teodor , Harvanová Denisa , Ižariková Gabriela , Danišovič Ľuboš

Identyfikatory

DOI

10.37190/ABB-01407-2019-02

• Języki publikacji: EN

Abstrakty

EN

The objective of this study was to fabricate PLA-based porous scaffold by 3D printing technology and to evaluate their cytotoxicity and biocompatibility under in vitro conditions in respect to bone tissue engineering. Material and methods: Pure PLA in filamentous form was processed via 3D printing technology of fused filament fabrication into porous scaffolds. The structure and porosity of scaffolds were measured by metrotomography. PLA scaffolds were pre-treated by human serum, foetal bovine serum and complete cell culture medium to enhance bio-attractivity of the scaffold’s surface for the adherence of the cells. Cells were enzymatically isolated from the periosteum of the proximal tibia and then expanded in monolayer. Periosteum-derived osteoprogenitors (PDOs) were seeded on the pre-treated PLA scaffolds and subsequent cell proliferation was measured by commercially available cell proliferation assay. Adherence of PDOs on the PLA scaffold was confirmed by scanning electron microscopy (SEM). Results: Prepared scaffolds had well-defined structure and were characterized by uniform distribution of pores. They were non-toxic and biocompatible with PDOs, however, PLA scaffold with the periosteum-derived progenitor cells was significantly better in the group of scaffolds pre-treated with normal human serum. Conclusions: The obtained PLA porous scaffolds favored attachment of periosteum derived progenitors and proliferation, furthermore, cells penetrated into the scaffold through the interstitial pores which was meaningful for cytocompatibility evaluation.

Słowa kluczowe

EN

polylactic acid; PLA; 3D printing; porous scaffold; periosteum-derived osteoprogenitors; PDOs; tissue engineering

PL

kwas polimlekowy; PLA; drukowanie 3D; inżynieria tkankowa

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Acta of Bioengineering and Biomechanics
• Rocznik: 2019
• Tom: Vol. 21, no. 4
• Strony: 101--110
• Opis fizyczny: Bibliogr. 25 poz., rys., tab., wykr.

Twórcy

autor

Bodnárová Simona

• Department of Biomedical Engineering and Measurement, Faculty of Mechanical Engineering, Technical University of Kosice, Slovakia

autor

Gromošová Sylvia

• Associated Tissue Bank, Faculty of Medicine P. J. Safarik University and L. Pasteur University Hospital Kosice, Slovakia

autor

Hudák Radovan

• Department of Biomedical Engineering and Measurement, Faculty of Mechanical Engineering, Technical University of Kosice, Slovakia

autor

Rosocha Ján

• Associated Tissue Bank, Faculty of Medicine P. J. Safarik University and L. Pasteur University Hospital Kosice, Slovakia

autor

Živčák Jozef

• Department of Biomedical Engineering and Measurement, Faculty of Mechanical Engineering, Technical University of Kosice, Slovakia

autor

Plšíková Jana

• Associated Tissue Bank, Faculty of Medicine P. J. Safarik University and L. Pasteur University Hospital Kosice, Slovakia

autor

Vojtko Marek

• Institute of Materials Research, Slovak Academy of Sciences, Kosice, Slovakia

autor

Tóth Teodor

• Associated Tissue Bank, Faculty of Medicine P. J. Safarik University and L. Pasteur University Hospital Kosice, Slovakia

autor

Harvanová Denisa

• Department of Biomedical Engineering and Measurement, Faculty of Mechanical Engineering, Technical University of Kosice, Slovakia

autor

Ižariková Gabriela

• Department of Applied Mathematics and Informatics, Faculty of Mechanical Engineering, Technical University of Kosice, Slovakia

autor

Danišovič Ľuboš

• Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava, Slovakia
• Regenmed Ltd., Research Division, Bratislava, Slovakia

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).