A new method of determination of collagen conjugated with keratin

• Autorzy: Safandowska M. , Pietrucha K.

Identyfikatory

DOI

DOI: 10.2478/v10304-012-0024-6

• Języki publikacji: EN

Abstrakty

EN

The paper describes the possibility of using Sirius red dye for the determination of collagen conjugated with keratin of wool. Sirius red assay was shown to be feasible for collagen detection, which was enzymatically coupled onto wool fibers and woven fabric. The effectiveness of combination of keratin protein with collagen was evaluated.

Słowa kluczowe

EN

collagen; Sirius red; keratin

PL

kolagen; keratyna; grupa aminowa; pomiar

• Wydawca: Lodz University of Technology, Faculty of Material Technologies and Textile Design
• Czasopismo: Autex Research Journal
• Rocznik: 2013
• Tom: Vol. 13, no. 2
• Strony: 37--39
• Opis fizyczny: Bibliogr. 9 poz.

Twórcy

autor

Safandowska M.

• Department of Material and Commodity Sciences and Textile Metrology, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland

autor

Pietrucha K.

• Department of Material and Commodity Sciences and Textile Metrology, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland

Bibliografia

• [1] Lee C.H., Singla A., Lee Y., Biomedical applications of collagen, International Journal of Pharmaceutics 221, 1–22, 2001.
• [2] Zhu Y.; Gao C.; Liu X., Shen J.: Surface modification of polycaprolactone membrane via aminolysis and biomacromolecule immobilization for promoting cytocompatibility of human endothelial cells, Biomacromolecules 2, 1312-1319, 2002.
• [3] Jou C.H.; Lin S.M.; Yun L.; Hwang M.C.; Yu D.G.; Chou W.L.; Lee J.S.; Yang M.C.: Biofunctional properties of polyester fibres grafted with chitosan and collagen, Polymers for Advanced Technology 18, 235–239, 2007.
• [4] Pietrucha K., New collagen implant as dural substitute, Biomaterials 12, 320-323, 1991.
• [5] Carlson G. A., Dragoo J. L., Samimi B., Bruckner D. A., Bernard G. W., Hedrick M., Benhaim P., Bacteriostatic properties of biomatrices against common orthopaedic pathogens, Biochemical and biophysical Research Communications 321, 472-478, 2004.
• [6] Jus S.; Kokol V.; Guebitz G.M.: Tyrosinase-Catalysed coating of wool fibers different protein-based biomaterials, Journal of Biomaterials Science 20, 253-269, 2009.
• [7] Junquiera L.C.U.; Bignolas G.; Brentani R.R.: A simple and sensitive method for the quantitative estimation of collagen, Analytical Biochemistry 94, 96-99, 1979.
• [8] Jus S.: Kokol V.; Guebitz G.M.: Tyrosinase-catalysed coupling of functional molecules onto protein fibres, Enzyme and Microbial Technology 42, 535-542, 2008.
• [9] Thalmann C.R., Lotzbeyer T., Enzymatic cross-linking of proteins with tyrosinase, EUR Food Res Technol 214, 276-281, 2002.