Exploring the Bacillus from vegetable rhizosphere for plant growth

Risanti, Rara Rahmatika; Hindersah, Reginawanti; Fitriatin, Betty Natalie; Suryatmana, Pujawati; Maksum, Iman Permana; Setiawati, Mieke Rochimi; Hanindipto, Fasa Aditya; Nugraha, Gita Bina

doi:10.12911/22998993/195286

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Tytuł artykułu

Exploring the Bacillus from vegetable rhizosphere for plant growth

Autorzy

Risanti Rara Rahmatika , Hindersah Reginawanti , Fitriatin Betty Natalie , Suryatmana Pujawati , Maksum Iman Permana , Setiawati Mieke Rochimi , Hanindipto Fasa Aditya , Nugraha Gita Bina

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DOI

10.12911/22998993/195286

Warianty tytułu

Języki publikacji

Abstrakty

Bacillus is a ubiquitous soil bacterium for its plant growth-promoting properties, and it is widely used as a biofertilizer. This research aimed to isolate and determine the morphology and biochemical properties associated with plant-growth promotion ability characterizing Bacillus strains. The bacteria were isolated from the rhizosphere of various highland vegetables grown in Andisols soil in Indonesia. Bacillus isolation was conducted using the serial dilution plate method by means of tryptic soy agar. Characterization of the isolated bacteria included Gram staining, biochemical characteristics, pathogenicity testing, and the production of metabolites, including organic acids, phytohormones, and exopolysaccharides. Four bacterial isolates were identified as Bacillus based on colony and cell morphology, the presence of endospore, as well as biochemical properties and metabolite production. The species determination by PCR amplification and 16S rRNA gene sequence analysis revealed that the four Bacillus were identified as Bacillus safensis strain MDL5, Bacillus altitudinis strain RPW2, and Bacillus sp. strain SZ057. The study presents the occurrence of Bacillus within the rhizosphere of vegetables and the understanding of their properties being considered for biofertilizer development.

Słowa kluczowe

biochemical properties Bacillus exopolysaccharides organic acids phosphatase phytohormones plant growth promoting rhizobacteria

Wydawca

Polskie Towarzystwo Inżynierii Ekologicznej
Lublin University of Technology

Czasopismo

Journal of Ecological Engineering

Rocznik

2025

Tom

Vol. 26, nr 1

Strony

109--120

Opis fizyczny

Bibliogr. 53 poz., rys., tab.

Twórcy

autor

Risanti Rara Rahmatika

rara17003@mail.unpad.ac.id

Student Doctoral Program, Faculty of Agriculture, Universitas Padjadjaran, Sumedang 45363, Indonesia

https://orcid.org/0000-0002-1619-6234

autor

Hindersah Reginawanti

reginawanti@unpad.ac.id

Department of Soil Science, Faculty of Agriculture, Universitas Padjadjaran, Sumedang 45363, Indonesia

https://orcid.org/0000-0003-0281-2363

autor

Fitriatin Betty Natalie

betty.natalie@unpad.ac.id

Department of Soil Science, Faculty of Agriculture, Universitas Padjadjaran, Sumedang 45363, Indonesia

https://orcid.org/0000-0002-3749-5081

autor

Suryatmana Pujawati

pujawati@unpad.ac.id

Department of Soil Science, Faculty of Agriculture, Universitas Padjadjaran, Sumedang 45363, Indonesia

https://orcid.org/0000-0002-4012-8645

autor

Maksum Iman Permana

iman.permana@unpad.ac.id

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Sumedang 45363, Indonesia

https://orcid.org/0000-0002-8018-2275

autor

Setiawati Mieke Rochimi

m.setiawati@unpad.ac.id

Department of Soil Science, Faculty of Agriculture, Universitas Padjadjaran, Sumedang 45363, Indonesia

https://orcid.org/0000-0001-5208-3141

autor

Hanindipto Fasa Aditya

Indonesian Fertilizer Research Institute, PT. Pupuk Indonesia, Jl. Taman Anggrek No. 2, Kemanggisan Jakarta Barat 11480, Indonesia

autor

Nugraha Gita Bina

Indonesian Fertilizer Research Institute, PT. Pupuk Indonesia, Jl. Taman Anggrek No. 2, Kemanggisan Jakarta Barat 11480, Indonesia

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